Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Carvalho, Augusto Marcelino Pedreira de
Data de Publicação: 2017
Outros Autores: Fukutani, Kiyoshi Ferreira, Sharma, Rohit, Curvelo, Rebecca P, Miranda, José Carlos, Barral, Aldina Maria Prado, Carvalho, Edgar Marcelino de, Valenzuela, Jesus G, Oliveira, Fabiano, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24773
Resumo: National Institutes of Health (AI 30639 to E.M.C.), by the Intramural Research Program of the NIH, National Institute of Allergy and Infectious Diseases (J.G.V., and F.O.), and by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (564689/2008-4 to C.I.O.). E.M.C., A.B. and C.I.O. are senior researchers at CNPq. A.M.C. received fellowship from Fundação de Amparo à Pesquisa da Bahia (FAPESB).
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spelling Carvalho, Augusto Marcelino Pedreira deFukutani, Kiyoshi FerreiraSharma, RohitCurvelo, Rebecca PMiranda, José CarlosBarral, Aldina Maria PradoCarvalho, Edgar Marcelino deValenzuela, Jesus GOliveira, FabianoOliveira, Camila Indiani de2018-02-08T13:19:13Z2018-02-08T13:19:13Z2017CARVALHO, A. M. P. et al. Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis. Scientific Reports, v. 7, p. 3149, 2017.2045-2322https://www.arca.fiocruz.br/handle/icict/2477310.1038/s41598-017-03345-0National Institutes of Health (AI 30639 to E.M.C.), by the Intramural Research Program of the NIH, National Institute of Allergy and Infectious Diseases (J.G.V., and F.O.), and by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (564689/2008-4 to C.I.O.). E.M.C., A.B. and C.I.O. are senior researchers at CNPq. A.M.C. received fellowship from Fundação de Amparo à Pesquisa da Bahia (FAPESB).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Program in Health Sciences. Salvador, BA, Brazil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Program in Health Sciences. Salvador, BA, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Program in Health Sciences. Salvador, BA, Brazil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Program in Health Sciences. Salvador, BA, Brazil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilSand flies inject saliva while feeding in the vertebrate host and anti-saliva antibodies can be used as biomarkers of exposure to Leishmania vectors. We expressed recombinant salivary proteins from Lutzomyia intermedia, a vector of Leishmania braziliensis, and evaluated the seroreactivity in exposed individuals in search for exposure markers. We found a strong correlation among positive serology to recombinant proteins LinB-13, 26, 15, 21 and to salivary proteins: rLinB-13 was the top performing molecule; IgG4 was the most predominant antibody subclass and antibodies to rLinB-13 did not cross react with Lu. longipalpis salivary proteins. By evaluating a cohort of contacts of CL patients, we confirmed that rLinB-13, an antigen 5-related protein, is a marker of exposure to Lu. intermedia with high degree of accuracy. In a 5-year follow up, we determined that individuals who developed CL presented higher anti-rLinB13 IgG responses, before the appearance of clinical symptoms. They also presented a lower frequency of cellular responses to the parasite (DTH). Our results show that seroconversion to a salivary molecule, rLinB-13, is a marker of risk for CL development caused by Leishmania braziliensis. This highlight the possibility of developing tools based on vector molecules to manage the disease in endemic areas.engNature Publishing GroupLutzomyiaLeishmania braziliensisBiomarcadoresProteínas salivaresLutzomyiaLeishmania braziliensisBiomarkersSalivary proteinsSeroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24773/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCarvalho AM Seroconversion to Lutzomyia intermedia LinB-13.........pdfCarvalho AM Seroconversion to Lutzomyia intermedia 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dc.title.pt_BR.fl_str_mv Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
title Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
spellingShingle Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
Carvalho, Augusto Marcelino Pedreira de
Lutzomyia
Leishmania braziliensis
Biomarcadores
Proteínas salivares
Lutzomyia
Leishmania braziliensis
Biomarkers
Salivary proteins
title_short Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
title_full Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
title_fullStr Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
title_full_unstemmed Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
title_sort Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis
author Carvalho, Augusto Marcelino Pedreira de
author_facet Carvalho, Augusto Marcelino Pedreira de
Fukutani, Kiyoshi Ferreira
Sharma, Rohit
Curvelo, Rebecca P
Miranda, José Carlos
Barral, Aldina Maria Prado
Carvalho, Edgar Marcelino de
Valenzuela, Jesus G
Oliveira, Fabiano
Oliveira, Camila Indiani de
author_role author
author2 Fukutani, Kiyoshi Ferreira
Sharma, Rohit
Curvelo, Rebecca P
Miranda, José Carlos
Barral, Aldina Maria Prado
Carvalho, Edgar Marcelino de
Valenzuela, Jesus G
Oliveira, Fabiano
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Augusto Marcelino Pedreira de
Fukutani, Kiyoshi Ferreira
Sharma, Rohit
Curvelo, Rebecca P
Miranda, José Carlos
Barral, Aldina Maria Prado
Carvalho, Edgar Marcelino de
Valenzuela, Jesus G
Oliveira, Fabiano
Oliveira, Camila Indiani de
dc.subject.other.pt_BR.fl_str_mv Lutzomyia
Leishmania braziliensis
Biomarcadores
Proteínas salivares
topic Lutzomyia
Leishmania braziliensis
Biomarcadores
Proteínas salivares
Lutzomyia
Leishmania braziliensis
Biomarkers
Salivary proteins
dc.subject.en.pt_BR.fl_str_mv Lutzomyia
Leishmania braziliensis
Biomarkers
Salivary proteins
description National Institutes of Health (AI 30639 to E.M.C.), by the Intramural Research Program of the NIH, National Institute of Allergy and Infectious Diseases (J.G.V., and F.O.), and by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (564689/2008-4 to C.I.O.). E.M.C., A.B. and C.I.O. are senior researchers at CNPq. A.M.C. received fellowship from Fundação de Amparo à Pesquisa da Bahia (FAPESB).
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-08T13:19:13Z
dc.date.available.fl_str_mv 2018-02-08T13:19:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, A. M. P. et al. Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis. Scientific Reports, v. 7, p. 3149, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24773
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-017-03345-0
identifier_str_mv CARVALHO, A. M. P. et al. Seroconversion to Lutzomyia intermedia LinB-13 as a biomarker for developing cutaneous leishmaniasis. Scientific Reports, v. 7, p. 3149, 2017.
2045-2322
10.1038/s41598-017-03345-0
url https://www.arca.fiocruz.br/handle/icict/24773
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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