Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection

Detalhes bibliográficos
Autor(a) principal: Gomes, Regis Bernardo Brandim
Data de Publicação: 2016
Outros Autores: Cavalcanti, Katrine Bezerra, Teixeira, Clarissa, Carvalho, Augusto Marcelino Pedreira de, Mattos, Paulo Sérgio de Morais da Silveira, Cristal, Juqueline Rocha, Muniz, Aline C, Miranda, José Carlos, Oliveira, Camila Indiani de, Barral, Aldina Maria Prado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18145
Resumo: Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq - 400695/2012-0) and Fundação de Amparo à Pesquisa da Bahia (FAPESB INT 0003/2013), Brazil.
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spelling Gomes, Regis Bernardo BrandimCavalcanti, Katrine BezerraTeixeira, ClarissaCarvalho, Augusto Marcelino Pedreira deMattos, Paulo Sérgio de Morais da SilveiraCristal, Juqueline RochaMuniz, Aline CMiranda, José CarlosOliveira, Camila Indiani deBarral, Aldina Maria Prado2017-03-23T17:10:59Z2017-03-23T17:10:59Z2016GOMES, R. B. B. et al. Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. PLoS Neglected Tropical Diseases, v. 10, n. 11, p. e0005078, 2016.1935-2727https://www.arca.fiocruz.br/handle/icict/1814510.1371/journal.pntd.0005078Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq - 400695/2012-0) and Fundação de Amparo à Pesquisa da Bahia (FAPESB INT 0003/2013), Brazil.Fundação Oswaldo Cruz (FIOCRUZ). Escritório Técnico Regional. Teresina, PI, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz (FIOCRUZ). Escritório Técnico Regional. Teresina, PI, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. School of Medicine. Salvador, BA, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / INCT. Instituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / INCT. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilPrevious works showed that immunization with saliva from Lutzomyia intermedia, a vector of Leishmania braziliensis, does not protect against experimental infection. However, L. braziliensis is also transmitted by Lutzomyia whitmani, a sand fly species closely related to Lu. intermedia. Herein we describe the immune response following immunization with Lu. whitmani saliva and the outcome of this response after L. braziliensis infection. Methods and findings BALB/c mice immunized with Lu. whitmani saliva developed robust humoral and cellular immune responses, the latter characterized by an intense cellular infiltrate and production of IFN-γ and IL-10, by both CD4+ and CD8+ cells. Mice immunized as above and challenged with L. braziliensis plus Lu. whitmani saliva displayed significantly smaller lesions and parasite load at the challenge site. This protection was associated with a higher (p<0.05) IFN-γ production in response to SLA stimulation. Long-term persisting immunity was also detected in mice immunized with Lu. whitmani saliva. Furthermore, individuals residing in an endemic area for cutaneous leishmaniasis (CL) presented antibody responses to Lu. whitmani saliva. However CL patients, with active lesions, displayed a lower humoral response to Lu. whitmani saliva compared to individuals with subclinical Leishmania infection. Conclusion Pre-exposure to Lu. whitmani saliva induces protection against L. braziliensis in a murine model. We also show that Lu. whitmani salivary proteins are immunogenic in naturally exposed individuals. Our results reinforce the importance of investigating the immunomodulatory effect of saliva from different species of closely related sand flies.engPublic Library of ScienceSalivaMoscasLutzomyiaLeishmania braziliensisInfecçãoProteinas salivaresSalivaSandLutzomyiaLeishmania braziliensisInfectionSalivary proteinsImmunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18145/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGomes R Immunity to Lutzomyia..,.pdfGomes R Immunity to 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dc.title.pt_BR.fl_str_mv Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection
title Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection
spellingShingle Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection
Gomes, Regis Bernardo Brandim
Saliva
Moscas
Lutzomyia
Leishmania braziliensis
Infecção
Proteinas salivares
Saliva
Sand
Lutzomyia
Leishmania braziliensis
Infection
Salivary proteins
title_short Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection
title_full Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection
title_fullStr Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection
title_full_unstemmed Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection
title_sort Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection
author Gomes, Regis Bernardo Brandim
author_facet Gomes, Regis Bernardo Brandim
Cavalcanti, Katrine Bezerra
Teixeira, Clarissa
Carvalho, Augusto Marcelino Pedreira de
Mattos, Paulo Sérgio de Morais da Silveira
Cristal, Juqueline Rocha
Muniz, Aline C
Miranda, José Carlos
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
author_role author
author2 Cavalcanti, Katrine Bezerra
Teixeira, Clarissa
Carvalho, Augusto Marcelino Pedreira de
Mattos, Paulo Sérgio de Morais da Silveira
Cristal, Juqueline Rocha
Muniz, Aline C
Miranda, José Carlos
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Regis Bernardo Brandim
Cavalcanti, Katrine Bezerra
Teixeira, Clarissa
Carvalho, Augusto Marcelino Pedreira de
Mattos, Paulo Sérgio de Morais da Silveira
Cristal, Juqueline Rocha
Muniz, Aline C
Miranda, José Carlos
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
dc.subject.other.pt_BR.fl_str_mv Saliva
Moscas
Lutzomyia
Leishmania braziliensis
Infecção
Proteinas salivares
topic Saliva
Moscas
Lutzomyia
Leishmania braziliensis
Infecção
Proteinas salivares
Saliva
Sand
Lutzomyia
Leishmania braziliensis
Infection
Salivary proteins
dc.subject.en.pt_BR.fl_str_mv Saliva
Sand
Lutzomyia
Leishmania braziliensis
Infection
Salivary proteins
description Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq - 400695/2012-0) and Fundação de Amparo à Pesquisa da Bahia (FAPESB INT 0003/2013), Brazil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-23T17:10:59Z
dc.date.available.fl_str_mv 2017-03-23T17:10:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOMES, R. B. B. et al. Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. PLoS Neglected Tropical Diseases, v. 10, n. 11, p. e0005078, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18145
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0005078
identifier_str_mv GOMES, R. B. B. et al. Immunity to Lutzomyia whitmani Saliva Protects against Experimental Leishmania braziliensis Infection. PLoS Neglected Tropical Diseases, v. 10, n. 11, p. e0005078, 2016.
1935-2727
10.1371/journal.pntd.0005078
url https://www.arca.fiocruz.br/handle/icict/18145
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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