Profile of Central and Effector Memory T Cells in the Progression of Chronic Human Chagas Disease

Detalhes bibliográficos
Autor(a) principal: Fiuza, Jacqueline Araujo
Data de Publicação: 2009
Outros Autores: Fujiwara, Ricardo Toshio, Gomes, Juliana Assis Silva, Rocha, Manoel Otávio das Costa, Chaves, Ana Thereza, Araújo, Fernanda Fortes de, Fares, Rafaelle Christine Gomes, Carvalho, Andrea Teixeira de, Martins Filho, Olindo de Assis, Cançado, Guilherme Grossi Lopes, Oliveira, Rodrigo Correa de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59245
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Santa Casa de Misericórdia de Belo Horizonte. Pós-graduação em Biomedicina e Clínica Médica. Belo Horizonte, MG, Brazil
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spelling Fiuza, Jacqueline AraujoFujiwara, Ricardo ToshioGomes, Juliana Assis SilvaRocha, Manoel Otávio das CostaChaves, Ana TherezaAraújo, Fernanda Fortes deFares, Rafaelle Christine GomesCarvalho, Andrea Teixeira deMartins Filho, Olindo de AssisCançado, Guilherme Grossi LopesOliveira, Rodrigo Correa de2023-06-29T12:50:20Z2023-06-29T12:50:20Z2009FIUZA, Jacqueline Araújo et al. Profile of central and effector memory T cells in the progression of chronic human chagas disease. PLoS Negl Trop Dis., v. 3, n. 9, e512, 2009. doi: 10.1371/journal.pntd.0000512.1935-2735https://www.arca.fiocruz.br/handle/icict/5924510.1371/journal.pntd.0000512engPublic Library of ScienceProfile of Central and Effector Memory T Cells in the Progression of Chronic Human Chagas Diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Santa Casa de Misericórdia de Belo Horizonte. Pós-graduação em Biomedicina e Clínica Médica. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-graduação em Ciências da Saúde: Infectologia e Medicina Tropical. Belo Horizonte, MG, Brazil/New York University School of Medicine. New York, NY, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Santa Casa de Misericórdia de Belo Horizonte. Pós-graduação em Biomedicina e Clínica Médica. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilBackground: Chronic Chagas disease presents several different clinical manifestations ranging from asymptomatic to severe cardiac and/or digestive clinical forms. Several studies have demonstrated that immunoregulatory mechanisms are important processes for the control of the intense immune activity observed in the chronic phase. T cells play a critical role in parasite specific and non-specific immune response elicited by the host against Trypanosoma cruzi. Specifically, memory T cells, which are basically classified as central and effector memory cells, might have a distinct migratory activity, role and function during the human Chagas disease. Methodology/Principal Findings: Based on the hypothesis that the disease severity in humans is correlated to the quality of immune responses against T. cruzi, we evaluated the memory profile of peripheral CD4(+) and CD8(+) T lymphocytes as well as its cytokine secretion before and after in vitro antigenic stimulation. We evaluated cellular response from non-infected individuals (NI), patients with indeterminate (IND) or cardiac (CARD) clinical forms of Chagas disease. The expression of CD45RA, CD45RO and CCR7 surface molecules was determined on CD4(+) and CD8(+) T lymphocytes; the pattern of intracellular cytokines (IFN-gamma, IL-10) synthesized by naive and memory cells was determined by flow cytometry. Our results revealed that IND and CARD patients have relatively lower percentages of naive (CD45RA(high)) CD4(+) and CD8(+) T cells. However, statistical analysis of ex-vivo profiles of CD4(+) T cells showed that IND have lower percentage of CD45RA(high) in relation to non-infected individuals, but not in relation to CARD. Elevated percentages of memory (CD45RO(high)) CD4(+) T cells were also demonstrated in infected individuals, although statistically significant differences were only observed between IND and NI groups. Furthermore, when we analyzed the profile of secreted cytokines, we observed that CARD patients presented a significantly higher percentage of CD8(+)CD45RA(high) IFN-gamma-producing cells in control cultures and after antigen pulsing with soluble epimastigote antigens. Conclusions: Based on a correlation between the frequency of IFN-gamma producing CD8(+) T cells in the T cell memory compartment and the chronic chagasic myocarditis, we propose that memory T cells can be involved in the induction of the development of the severe clinical forms of the Chagas disease by mechanisms modulated by IFN-gamma. Furthermore, we showed that individuals from IND group presented more T-CM CD4(+) T cells, which may induce a regulatory mechanism to protect the host against the exacerbated inflammatory response elicited by the infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALpntd.0000512 1..9.pdfpntd.0000512 1..9.pdfapplication/pdf279718https://www.arca.fiocruz.br/bitstream/icict/59245/2/pntd.0000512%201..9.pdf051599589c935bb9dbb8e90e9f560b68MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59245/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/592452023-06-29 09:50:21.35oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-06-29T12:50:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Profile of Central and Effector Memory T Cells in the Progression of Chronic Human Chagas Disease
title Profile of Central and Effector Memory T Cells in the Progression of Chronic Human Chagas Disease
spellingShingle Profile of Central and Effector Memory T Cells in the Progression of Chronic Human Chagas Disease
Fiuza, Jacqueline Araujo
title_short Profile of Central and Effector Memory T Cells in the Progression of Chronic Human Chagas Disease
title_full Profile of Central and Effector Memory T Cells in the Progression of Chronic Human Chagas Disease
title_fullStr Profile of Central and Effector Memory T Cells in the Progression of Chronic Human Chagas Disease
title_full_unstemmed Profile of Central and Effector Memory T Cells in the Progression of Chronic Human Chagas Disease
title_sort Profile of Central and Effector Memory T Cells in the Progression of Chronic Human Chagas Disease
author Fiuza, Jacqueline Araujo
author_facet Fiuza, Jacqueline Araujo
Fujiwara, Ricardo Toshio
Gomes, Juliana Assis Silva
Rocha, Manoel Otávio das Costa
Chaves, Ana Thereza
Araújo, Fernanda Fortes de
Fares, Rafaelle Christine Gomes
Carvalho, Andrea Teixeira de
Martins Filho, Olindo de Assis
Cançado, Guilherme Grossi Lopes
Oliveira, Rodrigo Correa de
author_role author
author2 Fujiwara, Ricardo Toshio
Gomes, Juliana Assis Silva
Rocha, Manoel Otávio das Costa
Chaves, Ana Thereza
Araújo, Fernanda Fortes de
Fares, Rafaelle Christine Gomes
Carvalho, Andrea Teixeira de
Martins Filho, Olindo de Assis
Cançado, Guilherme Grossi Lopes
Oliveira, Rodrigo Correa de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fiuza, Jacqueline Araujo
Fujiwara, Ricardo Toshio
Gomes, Juliana Assis Silva
Rocha, Manoel Otávio das Costa
Chaves, Ana Thereza
Araújo, Fernanda Fortes de
Fares, Rafaelle Christine Gomes
Carvalho, Andrea Teixeira de
Martins Filho, Olindo de Assis
Cançado, Guilherme Grossi Lopes
Oliveira, Rodrigo Correa de
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Santa Casa de Misericórdia de Belo Horizonte. Pós-graduação em Biomedicina e Clínica Médica. Belo Horizonte, MG, Brazil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2023-06-29T12:50:20Z
dc.date.available.fl_str_mv 2023-06-29T12:50:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FIUZA, Jacqueline Araújo et al. Profile of central and effector memory T cells in the progression of chronic human chagas disease. PLoS Negl Trop Dis., v. 3, n. 9, e512, 2009. doi: 10.1371/journal.pntd.0000512.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59245
dc.identifier.issn.en_US.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0000512
identifier_str_mv FIUZA, Jacqueline Araújo et al. Profile of central and effector memory T cells in the progression of chronic human chagas disease. PLoS Negl Trop Dis., v. 3, n. 9, e512, 2009. doi: 10.1371/journal.pntd.0000512.
1935-2735
10.1371/journal.pntd.0000512
url https://www.arca.fiocruz.br/handle/icict/59245
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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