Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection

Detalhes bibliográficos
Autor(a) principal: Mendonça, Pedro Henrique Braz
Data de Publicação: 2017
Outros Autores: Rocha, Raphael Francisco Dutra Barbosa da, Moraes, Julliane Brito de Braz, Freitas, Isabel Ferreira La Rocque de, Logullo, Jorgete, Morrot, Alexandre, Nunes, Marise Pinheiro, Lima, Celio Geraldo Freire de, Ricardo, Debora Decote
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19724
Resumo: Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.
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spelling Mendonça, Pedro Henrique BrazRocha, Raphael Francisco Dutra Barbosa daMoraes, Julliane Brito de BrazFreitas, Isabel Ferreira La Rocque deLogullo, JorgeteMorrot, AlexandreNunes, Marise PinheiroLima, Celio Geraldo Freire deRicardo, Debora Decote2017-07-05T14:12:11Z2017-07-05T14:12:11Z2017MENDONÇA, Pedro Henrique Braz; et al. Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection. Front Immunol., v.8:604, 11p, May 2017.1664-3224https://www.arca.fiocruz.br/handle/icict/1972410.3389/fimmu.2017.00604engFrontiers MediaTrypanosoma cruziDoença de ChagasCãesMacrófagosImnomodulaçãoSusceptibilidadeModelo de infecçãoTrypanosoma cruziChagas DiseaseDogsMachophagesimmunomodulationinfection modelsusceptibilityCanine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.Trypanosoma cruzi is an obligatory intracellular protozoan parasite, and it is the etiological agent of Chagas' disease that is endemic in the Americas. In addition to humans, a wide spectrum of mammals can be infected by T. cruzi, including dogs. Dogs develop acute and chronic disease, similar to human infection. T. cruzi can infect almost all cell types and after cell invasion, the metacyclics trypomastigotes localize in the cytoplasm, where they transform into amastigotes, the replicative form of T. cruzi in mammals. After amastigote multiplication and differentiation, parasites lyse host cells and spread through the body by blood circulation. In this work, we evaluated the in vitro ability of T. cruzi to infect a canine macrophage cell line DH82 compared with RAW264.7, a murine tissue culture macrophage. Our results have shown that the T. cruzi is able to infect, replicate and differentiate in DH82 cell line. We observed that following treatment with LPS and IFN-γ DH82 cells were more resistant to infection and that resistance was not related reactive oxygen species production in our system. In this study, we also found that DH82 cells became more susceptible to T. cruzi infection when cocultured with apoptotic cells. The analysis of cytokine production has showed elevated levels of the TGF-β, IL-10, and TNF-α produced by T. cruzi-infected canine macrophages. Additionally, we demonstrated a reduced expression of the MHC class II and CD80 by infected DH82 cell line.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19724/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmarise_nunes_etal_IOC_2017.pdfmarise_nunes_etal_IOC_2017.pdfapplication/pdf1246095https://www.arca.fiocruz.br/bitstream/icict/19724/2/marise_nunes_etal_IOC_2017.pdf548f464ca2e48448d7a0f90ad7b62711MD52TEXTmarise_nunes_etal_IOC_2017.pdf.txtmarise_nunes_etal_IOC_2017.pdf.txtExtracted texttext/plain52883https://www.arca.fiocruz.br/bitstream/icict/19724/3/marise_nunes_etal_IOC_2017.pdf.txtfadd9376107e738919f6963cf211bb8cMD53icict/197242022-06-24 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dc.title.pt_BR.fl_str_mv Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection
title Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection
spellingShingle Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection
Mendonça, Pedro Henrique Braz
Trypanosoma cruzi
Doença de Chagas
Cães
Macrófagos
Imnomodulação
Susceptibilidade
Modelo de infecção
Trypanosoma cruzi
Chagas Disease
Dogs
Machophages
immunomodulation
infection model
susceptibility
title_short Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection
title_full Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection
title_fullStr Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection
title_full_unstemmed Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection
title_sort Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection
author Mendonça, Pedro Henrique Braz
author_facet Mendonça, Pedro Henrique Braz
Rocha, Raphael Francisco Dutra Barbosa da
Moraes, Julliane Brito de Braz
Freitas, Isabel Ferreira La Rocque de
Logullo, Jorgete
Morrot, Alexandre
Nunes, Marise Pinheiro
Lima, Celio Geraldo Freire de
Ricardo, Debora Decote
author_role author
author2 Rocha, Raphael Francisco Dutra Barbosa da
Moraes, Julliane Brito de Braz
Freitas, Isabel Ferreira La Rocque de
Logullo, Jorgete
Morrot, Alexandre
Nunes, Marise Pinheiro
Lima, Celio Geraldo Freire de
Ricardo, Debora Decote
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mendonça, Pedro Henrique Braz
Rocha, Raphael Francisco Dutra Barbosa da
Moraes, Julliane Brito de Braz
Freitas, Isabel Ferreira La Rocque de
Logullo, Jorgete
Morrot, Alexandre
Nunes, Marise Pinheiro
Lima, Celio Geraldo Freire de
Ricardo, Debora Decote
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de Chagas
Cães
Macrófagos
Imnomodulação
Susceptibilidade
Modelo de infecção
topic Trypanosoma cruzi
Doença de Chagas
Cães
Macrófagos
Imnomodulação
Susceptibilidade
Modelo de infecção
Trypanosoma cruzi
Chagas Disease
Dogs
Machophages
immunomodulation
infection model
susceptibility
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chagas Disease
Dogs
Machophages
immunomodulation
infection model
susceptibility
description Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-07-05T14:12:11Z
dc.date.available.fl_str_mv 2017-07-05T14:12:11Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MENDONÇA, Pedro Henrique Braz; et al. Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection. Front Immunol., v.8:604, 11p, May 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19724
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2017.00604
identifier_str_mv MENDONÇA, Pedro Henrique Braz; et al. Canine Macrophage DH82 Cell Line As a Model to Study Susceptibility to Trypanosoma cruzi Infection. Front Immunol., v.8:604, 11p, May 2017.
1664-3224
10.3389/fimmu.2017.00604
url https://www.arca.fiocruz.br/handle/icict/19724
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/19724/2/marise_nunes_etal_IOC_2017.pdf
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