Comparison of four molecular approaches to identify Candida parapsilosis complex species

Detalhes bibliográficos
Autor(a) principal: Barbedo, Leonardo Silva
Data de Publicação: 2017
Outros Autores: Figueiredo-Carvalho, Maria Helena Galdino, Muniz, Mauro de Medeiros, Zancopé-Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31274
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
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spelling Barbedo, Leonardo SilvaFigueiredo-Carvalho, Maria Helena GaldinoMuniz, Mauro de MedeirosZancopé-Oliveira, Rosely Maria2019-01-24T11:18:48Z2019-01-24T11:18:48Z2017BARBEDO, Leonardo Silva et al. Comparison of four molecular approaches to identify Candida parapsilosis complex species. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 3, p. 214-219, Mar. 2017.0074-0276https://www.arca.fiocruz.br/handle/icict/3127410.1590/0074-027601604121678-8060engInstituto Oswaldo CruzComparison of four molecular approaches to identify Candida parapsilosis complex speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Since the description of Candida orthopsilosis and C. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from C. parapsilosis sensu stricto. Species-specific uniplex polymerase chain reaction (PCR) was performed and compared with sequencing of the D1/D2 region of the LSU 28S rDNA gene, microsatellite typing of C. parapsilosis sensu stricto, and PCR-restriction fragment length polymorphism patterns in the ITS1-5.8S-ITS2 region of the rDNA gene. There was agreement between results of testing of 98 clinical isolates with the four PCR-based methods, with 59 isolates identified as C. parapsilosis sensu stricto, 37 as C. orthopsilosis, and two as C. metapsilosis.Candida parapsilosis complexCandida orthopsilosisCandida metapsilosisCandidaemiaDNAPCRinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31274/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Barbedo_Leonardo_etal_INI_2017.pdfve_Barbedo_Leonardo_etal_INI_2017.pdfapplication/pdf420611https://www.arca.fiocruz.br/bitstream/icict/31274/2/ve_Barbedo_Leonardo_etal_INI_2017.pdf6f600dac2d622507f5322e517969c628MD52TEXTve_Barbedo_Leonardo_etal_INI_2017.pdf.txtve_Barbedo_Leonardo_etal_INI_2017.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Comparison of four molecular approaches to identify Candida parapsilosis complex species
title Comparison of four molecular approaches to identify Candida parapsilosis complex species
spellingShingle Comparison of four molecular approaches to identify Candida parapsilosis complex species
Barbedo, Leonardo Silva
Candida parapsilosis complex
Candida orthopsilosis
Candida metapsilosis
Candidaemia
DNA
PCR
title_short Comparison of four molecular approaches to identify Candida parapsilosis complex species
title_full Comparison of four molecular approaches to identify Candida parapsilosis complex species
title_fullStr Comparison of four molecular approaches to identify Candida parapsilosis complex species
title_full_unstemmed Comparison of four molecular approaches to identify Candida parapsilosis complex species
title_sort Comparison of four molecular approaches to identify Candida parapsilosis complex species
author Barbedo, Leonardo Silva
author_facet Barbedo, Leonardo Silva
Figueiredo-Carvalho, Maria Helena Galdino
Muniz, Mauro de Medeiros
Zancopé-Oliveira, Rosely Maria
author_role author
author2 Figueiredo-Carvalho, Maria Helena Galdino
Muniz, Mauro de Medeiros
Zancopé-Oliveira, Rosely Maria
author2_role author
author
author
dc.contributor.author.fl_str_mv Barbedo, Leonardo Silva
Figueiredo-Carvalho, Maria Helena Galdino
Muniz, Mauro de Medeiros
Zancopé-Oliveira, Rosely Maria
dc.subject.en.pt_BR.fl_str_mv Candida parapsilosis complex
Candida orthopsilosis
Candida metapsilosis
Candidaemia
DNA
PCR
topic Candida parapsilosis complex
Candida orthopsilosis
Candida metapsilosis
Candidaemia
DNA
PCR
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2019-01-24T11:18:48Z
dc.date.available.fl_str_mv 2019-01-24T11:18:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBEDO, Leonardo Silva et al. Comparison of four molecular approaches to identify Candida parapsilosis complex species. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 3, p. 214-219, Mar. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31274
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760160412
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BARBEDO, Leonardo Silva et al. Comparison of four molecular approaches to identify Candida parapsilosis complex species. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 3, p. 214-219, Mar. 2017.
0074-0276
10.1590/0074-02760160412
1678-8060
url https://www.arca.fiocruz.br/handle/icict/31274
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
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