Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7405 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil |
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Afonso, Lilian MariaBorges, Valeria de MatosCruz, HeloísaGomes, Flávia Lima RibeiroReis, George A. dosDutra, Alberto Augusto NoronhaClarêncio, JorgeOliveira, Camila Indiani deBarral, Aldina Maria PradoBarral Netto, ManoelBrodskyn, Claudia Ida2014-03-13T17:49:29Z2014-03-13T17:49:29Z2008AFONSO, L. Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis. Journal of Leukocyte Biology, v.84, n. 2, p.389-392, 2008.0741-5400https://www.arca.fiocruz.br/handle/icict/740510.1189/jlb.0108018engSociety for Leukocyte BiologyInteractions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, BrasilNeutrophils are involved in the initial steps of most responses to pathogens. In the present study, we evaluated the effects of the interaction of apoptotic vs. necrotic human neutrophils on macrophage infection by Leishmania amazonensis. Phagocytosis of apoptotic, but not viable, neutrophils by Leishmania-infected macrophages led to an increase in parasite burden via a mechanism dependent on TGF-beta1 and PGE2. Conversely, infected macrophages' uptake of necrotic neutrophils induced killing of L. amazonensis. Leishmanicidal activity was dependent on TNF-alpha and neutrophilic elastase. Nitric oxide was not involved in the killing of parasites, but the interaction of necrotic neutrophils with infected macrophages resulted in high superoxide production, a process reversed by catalase, an inhibitor of reactive oxygen intermediate production. Initial events after Leishmania infection involve interactions with neutrophils; we demonstrate that phagocytosis of these cells in an apoptotic or necrotic stage can influence the outcome of infection, driving either parasite survival or destruction.ApoptosisNecrosisPhagocytosisTGF- B1NELeishmaniose Cutânea/fisiopatologiaMacrófagos/parasitologiaNeutrófilos/imunologiaNeutrófilos/fisiologiaAnimaisApoptoseCatalase/farmacologiaEfeitos Psicossociais da DoençaDinoprostona/fisiologiaHumanosLeishmania mexicana/patogenicidadeLeishmania mexicana/fisiologiaLeishmaniose Cutânea/patologiaNecroseNeutrófilos/patologiaFagocitoseSuperóxidos/metabolismoFator de Crescimento Transformador beta1/fisiologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAfonso L Interactions with....pdfAfonso L Interactions with....pdfapplication/pdf461168https://www.arca.fiocruz.br/bitstream/icict/7405/1/Afonso%20L%20Interactions%20with....pdfd58a0fa4a1b8c4672a4dc606f788780cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7405/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTAfonso L Interactions with....pdf.txtAfonso L Interactions with....pdf.txtExtracted texttext/plain45299https://www.arca.fiocruz.br/bitstream/icict/7405/5/Afonso%20L%20Interactions%20with....pdf.txtffa7edfd2208849eee007268774ef998MD55THUMBNAILAfonso L Interactions with....pdf.jpgAfonso L Interactions with....pdf.jpgGenerated Thumbnailimage/jpeg2606https://www.arca.fiocruz.br/bitstream/icict/7405/4/Afonso%20L%20Interactions%20with....pdf.jpge7023b943083a4f9fe5e933673db3603MD54icict/74052023-03-15 14:33:24.56oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis |
title |
Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis |
spellingShingle |
Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis Afonso, Lilian Maria Apoptosis Necrosis Phagocytosis TGF- B1 NE Leishmaniose Cutânea/fisiopatologia Macrófagos/parasitologia Neutrófilos/imunologia Neutrófilos/fisiologia Animais Apoptose Catalase/farmacologia Efeitos Psicossociais da Doença Dinoprostona/fisiologia Humanos Leishmania mexicana/patogenicidade Leishmania mexicana/fisiologia Leishmaniose Cutânea/patologia Necrose Neutrófilos/patologia Fagocitose Superóxidos/metabolismo Fator de Crescimento Transformador beta1/fisiologia |
title_short |
Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis |
title_full |
Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis |
title_fullStr |
Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis |
title_full_unstemmed |
Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis |
title_sort |
Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis |
author |
Afonso, Lilian Maria |
author_facet |
Afonso, Lilian Maria Borges, Valeria de Matos Cruz, Heloísa Gomes, Flávia Lima Ribeiro Reis, George A. dos Dutra, Alberto Augusto Noronha Clarêncio, Jorge Oliveira, Camila Indiani de Barral, Aldina Maria Prado Barral Netto, Manoel Brodskyn, Claudia Ida |
author_role |
author |
author2 |
Borges, Valeria de Matos Cruz, Heloísa Gomes, Flávia Lima Ribeiro Reis, George A. dos Dutra, Alberto Augusto Noronha Clarêncio, Jorge Oliveira, Camila Indiani de Barral, Aldina Maria Prado Barral Netto, Manoel Brodskyn, Claudia Ida |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Afonso, Lilian Maria Borges, Valeria de Matos Cruz, Heloísa Gomes, Flávia Lima Ribeiro Reis, George A. dos Dutra, Alberto Augusto Noronha Clarêncio, Jorge Oliveira, Camila Indiani de Barral, Aldina Maria Prado Barral Netto, Manoel Brodskyn, Claudia Ida |
dc.subject.en.pt_BR.fl_str_mv |
Apoptosis Necrosis Phagocytosis TGF- B1 NE |
topic |
Apoptosis Necrosis Phagocytosis TGF- B1 NE Leishmaniose Cutânea/fisiopatologia Macrófagos/parasitologia Neutrófilos/imunologia Neutrófilos/fisiologia Animais Apoptose Catalase/farmacologia Efeitos Psicossociais da Doença Dinoprostona/fisiologia Humanos Leishmania mexicana/patogenicidade Leishmania mexicana/fisiologia Leishmaniose Cutânea/patologia Necrose Neutrófilos/patologia Fagocitose Superóxidos/metabolismo Fator de Crescimento Transformador beta1/fisiologia |
dc.subject.decs.pt_BR.fl_str_mv |
Leishmaniose Cutânea/fisiopatologia Macrófagos/parasitologia Neutrófilos/imunologia Neutrófilos/fisiologia Animais Apoptose Catalase/farmacologia Efeitos Psicossociais da Doença Dinoprostona/fisiologia Humanos Leishmania mexicana/patogenicidade Leishmania mexicana/fisiologia Leishmaniose Cutânea/patologia Necrose Neutrófilos/patologia Fagocitose Superóxidos/metabolismo Fator de Crescimento Transformador beta1/fisiologia |
description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2014-03-13T17:49:29Z |
dc.date.available.fl_str_mv |
2014-03-13T17:49:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
AFONSO, L. Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis. Journal of Leukocyte Biology, v.84, n. 2, p.389-392, 2008. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/7405 |
dc.identifier.issn.none.fl_str_mv |
0741-5400 |
dc.identifier.doi.none.fl_str_mv |
10.1189/jlb.0108018 |
identifier_str_mv |
AFONSO, L. Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis. Journal of Leukocyte Biology, v.84, n. 2, p.389-392, 2008. 0741-5400 10.1189/jlb.0108018 |
url |
https://www.arca.fiocruz.br/handle/icict/7405 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Society for Leukocyte Biology |
publisher.none.fl_str_mv |
Society for Leukocyte Biology |
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Repositório Institucional da FIOCRUZ (ARCA) |
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