Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis

Detalhes bibliográficos
Autor(a) principal: Afonso, Lilian Maria
Data de Publicação: 2008
Outros Autores: Borges, Valeria de Matos, Cruz, Heloísa, Gomes, Flávia Lima Ribeiro, Reis, George A. dos, Dutra, Alberto Augusto Noronha, Clarêncio, Jorge, Oliveira, Camila Indiani de, Barral, Aldina Maria Prado, Barral Netto, Manoel, Brodskyn, Claudia Ida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7405
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
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spelling Afonso, Lilian MariaBorges, Valeria de MatosCruz, HeloísaGomes, Flávia Lima RibeiroReis, George A. dosDutra, Alberto Augusto NoronhaClarêncio, JorgeOliveira, Camila Indiani deBarral, Aldina Maria PradoBarral Netto, ManoelBrodskyn, Claudia Ida2014-03-13T17:49:29Z2014-03-13T17:49:29Z2008AFONSO, L. Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis. Journal of Leukocyte Biology, v.84, n. 2, p.389-392, 2008.0741-5400https://www.arca.fiocruz.br/handle/icict/740510.1189/jlb.0108018engSociety for Leukocyte BiologyInteractions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, BrasilNeutrophils are involved in the initial steps of most responses to pathogens. In the present study, we evaluated the effects of the interaction of apoptotic vs. necrotic human neutrophils on macrophage infection by Leishmania amazonensis. Phagocytosis of apoptotic, but not viable, neutrophils by Leishmania-infected macrophages led to an increase in parasite burden via a mechanism dependent on TGF-beta1 and PGE2. Conversely, infected macrophages' uptake of necrotic neutrophils induced killing of L. amazonensis. Leishmanicidal activity was dependent on TNF-alpha and neutrophilic elastase. Nitric oxide was not involved in the killing of parasites, but the interaction of necrotic neutrophils with infected macrophages resulted in high superoxide production, a process reversed by catalase, an inhibitor of reactive oxygen intermediate production. Initial events after Leishmania infection involve interactions with neutrophils; we demonstrate that phagocytosis of these cells in an apoptotic or necrotic stage can influence the outcome of infection, driving either parasite survival or destruction.ApoptosisNecrosisPhagocytosisTGF- B1NELeishmaniose Cutânea/fisiopatologiaMacrófagos/parasitologiaNeutrófilos/imunologiaNeutrófilos/fisiologiaAnimaisApoptoseCatalase/farmacologiaEfeitos Psicossociais da DoençaDinoprostona/fisiologiaHumanosLeishmania mexicana/patogenicidadeLeishmania mexicana/fisiologiaLeishmaniose Cutânea/patologiaNecroseNeutrófilos/patologiaFagocitoseSuperóxidos/metabolismoFator de Crescimento Transformador beta1/fisiologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAfonso L Interactions with....pdfAfonso L Interactions with....pdfapplication/pdf461168https://www.arca.fiocruz.br/bitstream/icict/7405/1/Afonso%20L%20Interactions%20with....pdfd58a0fa4a1b8c4672a4dc606f788780cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7405/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTAfonso L Interactions with....pdf.txtAfonso L Interactions with....pdf.txtExtracted texttext/plain45299https://www.arca.fiocruz.br/bitstream/icict/7405/5/Afonso%20L%20Interactions%20with....pdf.txtffa7edfd2208849eee007268774ef998MD55THUMBNAILAfonso L Interactions with....pdf.jpgAfonso L Interactions with....pdf.jpgGenerated Thumbnailimage/jpeg2606https://www.arca.fiocruz.br/bitstream/icict/7405/4/Afonso%20L%20Interactions%20with....pdf.jpge7023b943083a4f9fe5e933673db3603MD54icict/74052023-03-15 14:33:24.56oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis
title Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis
spellingShingle Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis
Afonso, Lilian Maria
Apoptosis
Necrosis
Phagocytosis
TGF- B1
NE
Leishmaniose Cutânea/fisiopatologia
Macrófagos/parasitologia
Neutrófilos/imunologia
Neutrófilos/fisiologia
Animais
Apoptose
Catalase/farmacologia
Efeitos Psicossociais da Doença
Dinoprostona/fisiologia
Humanos
Leishmania mexicana/patogenicidade
Leishmania mexicana/fisiologia
Leishmaniose Cutânea/patologia
Necrose
Neutrófilos/patologia
Fagocitose
Superóxidos/metabolismo
Fator de Crescimento Transformador beta1/fisiologia
title_short Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis
title_full Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis
title_fullStr Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis
title_full_unstemmed Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis
title_sort Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis
author Afonso, Lilian Maria
author_facet Afonso, Lilian Maria
Borges, Valeria de Matos
Cruz, Heloísa
Gomes, Flávia Lima Ribeiro
Reis, George A. dos
Dutra, Alberto Augusto Noronha
Clarêncio, Jorge
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
author_role author
author2 Borges, Valeria de Matos
Cruz, Heloísa
Gomes, Flávia Lima Ribeiro
Reis, George A. dos
Dutra, Alberto Augusto Noronha
Clarêncio, Jorge
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Afonso, Lilian Maria
Borges, Valeria de Matos
Cruz, Heloísa
Gomes, Flávia Lima Ribeiro
Reis, George A. dos
Dutra, Alberto Augusto Noronha
Clarêncio, Jorge
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
dc.subject.en.pt_BR.fl_str_mv Apoptosis
Necrosis
Phagocytosis
TGF- B1
NE
topic Apoptosis
Necrosis
Phagocytosis
TGF- B1
NE
Leishmaniose Cutânea/fisiopatologia
Macrófagos/parasitologia
Neutrófilos/imunologia
Neutrófilos/fisiologia
Animais
Apoptose
Catalase/farmacologia
Efeitos Psicossociais da Doença
Dinoprostona/fisiologia
Humanos
Leishmania mexicana/patogenicidade
Leishmania mexicana/fisiologia
Leishmaniose Cutânea/patologia
Necrose
Neutrófilos/patologia
Fagocitose
Superóxidos/metabolismo
Fator de Crescimento Transformador beta1/fisiologia
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose Cutânea/fisiopatologia
Macrófagos/parasitologia
Neutrófilos/imunologia
Neutrófilos/fisiologia
Animais
Apoptose
Catalase/farmacologia
Efeitos Psicossociais da Doença
Dinoprostona/fisiologia
Humanos
Leishmania mexicana/patogenicidade
Leishmania mexicana/fisiologia
Leishmaniose Cutânea/patologia
Necrose
Neutrófilos/patologia
Fagocitose
Superóxidos/metabolismo
Fator de Crescimento Transformador beta1/fisiologia
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2014-03-13T17:49:29Z
dc.date.available.fl_str_mv 2014-03-13T17:49:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AFONSO, L. Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis. Journal of Leukocyte Biology, v.84, n. 2, p.389-392, 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7405
dc.identifier.issn.none.fl_str_mv 0741-5400
dc.identifier.doi.none.fl_str_mv 10.1189/jlb.0108018
identifier_str_mv AFONSO, L. Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis. Journal of Leukocyte Biology, v.84, n. 2, p.389-392, 2008.
0741-5400
10.1189/jlb.0108018
url https://www.arca.fiocruz.br/handle/icict/7405
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for Leukocyte Biology
publisher.none.fl_str_mv Society for Leukocyte Biology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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