Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira

Detalhes bibliográficos
Autor(a) principal: Lüdtke, Charli Beatriz
Data de Publicação: 2003
Outros Autores: Coutinho, Mariana Loner, Jouglard, Sandra Denise Dorneles, Moreira, Cecília Nunes, Fernandes, Cláudia Hartleben Pinho, Brod, Claudiomar Soares, Haake, David A., Ko, Albert Icksang, Dellagostin, Odir Antonio, Aleixo, José Antonio Guimarães
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/6218
Resumo: Foram obtidos dois hibridomas secretores de anticorpos monoclonais (MAbs) que reagem com uma lipoproteína (LipL32) da membrana externa de leptospiras patogênicas. Para a produção dos hibridomas, células do baço de camundongos BALB/c, imunizados com LipL32 recombinante (rLipL32), foram fusionadas com células SP2/O-Ag14, selecionadas em meio HAT e testadas em ELISA indireto. Um dos MAbs secretados pelos hibridomas é do isotipo IgG2b e o outro do isotipo IgM. A especificidade dos MAbs foi confirmada em ELISA indireto e immunoblotting usando rLipL32 purificada, Escherichia coli (E. coli) expressando LipL32 e sorovares patogênicos e saprófitas. Os dois MAbs reagiram com a maioria dos sorovares patogênicos e não reagiram com sorovares saprófitas. Os MAbs possuem potencial para uso em testes de diagnóstico de leptospirose.
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spelling Lüdtke, Charli BeatrizCoutinho, Mariana LonerJouglard, Sandra Denise DornelesMoreira, Cecília NunesFernandes, Cláudia Hartleben PinhoBrod, Claudiomar SoaresHaake, David A.Ko, Albert IcksangDellagostin, Odir AntonioAleixo, José Antonio Guimarães2013-01-18T21:20:50Z2013-01-18T21:20:50Z2003LÜDTKE, C. B. et al. Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira. Brazilian Journal of Microbiology, v. 34, supl.1 p. 1-4, Nov. 2003.1517-8382https://www.arca.fiocruz.br/handle/icict/6218Foram obtidos dois hibridomas secretores de anticorpos monoclonais (MAbs) que reagem com uma lipoproteína (LipL32) da membrana externa de leptospiras patogênicas. Para a produção dos hibridomas, células do baço de camundongos BALB/c, imunizados com LipL32 recombinante (rLipL32), foram fusionadas com células SP2/O-Ag14, selecionadas em meio HAT e testadas em ELISA indireto. Um dos MAbs secretados pelos hibridomas é do isotipo IgG2b e o outro do isotipo IgM. A especificidade dos MAbs foi confirmada em ELISA indireto e immunoblotting usando rLipL32 purificada, Escherichia coli (E. coli) expressando LipL32 e sorovares patogênicos e saprófitas. Os dois MAbs reagiram com a maioria dos sorovares patogênicos e não reagiram com sorovares saprófitas. Os MAbs possuem potencial para uso em testes de diagnóstico de leptospirose.Two hybridomas secreting monoclonal antibodies (MAbs) that react with a lipoprotein (LipL32) of the outermembrane of pathogenic Leptospira were obtained. For hybridoma production, spleen cells from BALB/cmice imunized with recombinant LipL32 (rLipL32) were fused to SP2/O-Ag14 cells, selected in HAT mediumand screened in an indirect ELISA. One MAb produced was of the IgG2b isotype and the other was an IgM.MAbs specificity was confirmed by indirect ELISA and immunoblotting using purified rLipL32 and whole-cellantigen preparations from Escherichia coli (E. coli) expressing LipL32 and from pathogenic and non-pathogenic serovars. Both Mabs reacted with most of the pathogenic serovars tested and none reacted withnon-pathogenic Leptospira. The MAbs described have potential for use in diagnostic tests for leptospirosis.Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil.Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil.Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil.Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil.Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil.Universidade Federal de Pelotas. Centro de Controle de Zoonoses. Pelotas, RS, Brasil.UCLA School of Medicine. Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Diseases. New York, NY, USA.Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil.Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil.engMonoclonal antibodies against an outer membrane protein from pathogenic Leptospirainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeptospirosisMonoclonal antibodiesLipL32info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALLudtke, Charli Beatriz et al. Monoclonal antibodies....pdfLudtke, Charli Beatriz et al. 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dc.title.pt_BR.fl_str_mv Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
title Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
spellingShingle Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
Lüdtke, Charli Beatriz
Leptospirosis
Monoclonal antibodies
LipL32
title_short Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
title_full Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
title_fullStr Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
title_full_unstemmed Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
title_sort Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
author Lüdtke, Charli Beatriz
author_facet Lüdtke, Charli Beatriz
Coutinho, Mariana Loner
Jouglard, Sandra Denise Dorneles
Moreira, Cecília Nunes
Fernandes, Cláudia Hartleben Pinho
Brod, Claudiomar Soares
Haake, David A.
Ko, Albert Icksang
Dellagostin, Odir Antonio
Aleixo, José Antonio Guimarães
author_role author
author2 Coutinho, Mariana Loner
Jouglard, Sandra Denise Dorneles
Moreira, Cecília Nunes
Fernandes, Cláudia Hartleben Pinho
Brod, Claudiomar Soares
Haake, David A.
Ko, Albert Icksang
Dellagostin, Odir Antonio
Aleixo, José Antonio Guimarães
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lüdtke, Charli Beatriz
Coutinho, Mariana Loner
Jouglard, Sandra Denise Dorneles
Moreira, Cecília Nunes
Fernandes, Cláudia Hartleben Pinho
Brod, Claudiomar Soares
Haake, David A.
Ko, Albert Icksang
Dellagostin, Odir Antonio
Aleixo, José Antonio Guimarães
dc.subject.en.pt_BR.fl_str_mv Leptospirosis
Monoclonal antibodies
LipL32
topic Leptospirosis
Monoclonal antibodies
LipL32
description Foram obtidos dois hibridomas secretores de anticorpos monoclonais (MAbs) que reagem com uma lipoproteína (LipL32) da membrana externa de leptospiras patogênicas. Para a produção dos hibridomas, células do baço de camundongos BALB/c, imunizados com LipL32 recombinante (rLipL32), foram fusionadas com células SP2/O-Ag14, selecionadas em meio HAT e testadas em ELISA indireto. Um dos MAbs secretados pelos hibridomas é do isotipo IgG2b e o outro do isotipo IgM. A especificidade dos MAbs foi confirmada em ELISA indireto e immunoblotting usando rLipL32 purificada, Escherichia coli (E. coli) expressando LipL32 e sorovares patogênicos e saprófitas. Os dois MAbs reagiram com a maioria dos sorovares patogênicos e não reagiram com sorovares saprófitas. Os MAbs possuem potencial para uso em testes de diagnóstico de leptospirose.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2013-01-18T21:20:50Z
dc.date.available.fl_str_mv 2013-01-18T21:20:50Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv LÜDTKE, C. B. et al. Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira. Brazilian Journal of Microbiology, v. 34, supl.1 p. 1-4, Nov. 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6218
dc.identifier.issn.none.fl_str_mv 1517-8382
identifier_str_mv LÜDTKE, C. B. et al. Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira. Brazilian Journal of Microbiology, v. 34, supl.1 p. 1-4, Nov. 2003.
1517-8382
url https://www.arca.fiocruz.br/handle/icict/6218
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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