Embryonic development and egg viability of wMel-infected Aedes aegypti

Detalhes bibliográficos
Autor(a) principal: Farnesi, Luana Cristina
Data de Publicação: 2019
Outros Autores: Belinato, Thiago Affonso, Gesto, João Silveira Moledo, Martins, Ademir Jesus, Bruno, Rafaela Vieira, Moreira, Luciano Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35256
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil.
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spelling Farnesi, Luana CristinaBelinato, Thiago AffonsoGesto, João Silveira MoledoMartins, Ademir JesusBruno, Rafaela VieiraMoreira, Luciano Andrade2019-09-02T16:34:04Z2019-09-02T16:34:04Z2019FARNESI, Luana Cristina et al. Embryonic development and egg viability of wMel-infected Aedes aegypti. Parasites Vectors, v. 12, n. 211, p. 1-9, 2019.1756-3305https://www.arca.fiocruz.br/handle/icict/3525610.1186/s13071-019-3474-zengBMCAedes aegyptiWolbachiaEstirpe de wMelResistência à dessecaçãoEmbriogêneseViabilidade do ovoAedes aegyptiWolbachiawMel strainDesiccation resistanceEmbryogenesisEgg viabilityEmbryonic development and egg viability of wMel-infected Aedes aegyptiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG. Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Aedes aegypti is a major disease vector in urban habitats, involved in the transmission of dengue, chikungunya and Zika. Despite innumerous attempts to contain disease outbreaks, there are neither efficient vaccines nor definite vector control methods nowadays. In recent years, an innovative strategy to control arboviruses, which exploits the endosymbiotic bacterium Wolbachia pipientis, emerged with great expectations. The success of the method depends on many aspects, including Wolbachia's cytoplasmic incompatibility and pathogen interference phenotypes, as well as its effect on host fitness. In this work, we investigated the influence the Wolbachia strain wMel exerts on embryo development and egg viability and speculate on its field release use. Methods: Wild-type (Br or Rockefeller) and Wolbachia-harboring specimens (wMelBr) were blood-fed and submitted to synchronous egg laying for embryo development assays. Samples were analyzed for morphological markers, developmental endpoint and egg resistance to desiccation (ERD). Quiescent egg viability over time was also assessed. Results: wMelBr samples completed embryogenesis 2–3 hours later than wild-type. This delay was also observed through the onset of both morphological and physiological markers, respectively by the moments of germband extension and ERD acquisition. Following the end of embryonic development, wMelBr eggs were slightly less resistant to desiccation and showed reduced viability levels, which rapidly decayed after 40 days into quiescence, from approximately 75% to virtually 0% in less than a month. Conclusions: Our data revealed that the wMel strain of Wolbachia slightly delays embryogenesis and also afects egg quality, both through reduced viability and desiccation resistance. These fndings suggest that, although embryonic ftness is somehow compromised by wMel infection, an efcient host reproductive manipulation through cytoplasmic incompatibility seems sufcient to overcome these efects in nature and promote bacterial invasion, as shown by successful ongoing feld implementation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35256/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLuanaFarnesi_AdemirMartins_etal_IOC_2019.pdfLuanaFarnesi_AdemirMartins_etal_IOC_2019.pdfapplication/pdf1281280https://www.arca.fiocruz.br/bitstream/icict/35256/2/LuanaFarnesi_AdemirMartins_etal_IOC_2019.pdf7bb8964dc0d1e31716fab9f42a34928bMD52TEXTLuanaFarnesi_AdemirMartins_etal_IOC_2019.pdf.txtLuanaFarnesi_AdemirMartins_etal_IOC_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Embryonic development and egg viability of wMel-infected Aedes aegypti
title Embryonic development and egg viability of wMel-infected Aedes aegypti
spellingShingle Embryonic development and egg viability of wMel-infected Aedes aegypti
Farnesi, Luana Cristina
Aedes aegypti
Wolbachia
Estirpe de wMel
Resistência à dessecação
Embriogênese
Viabilidade do ovo
Aedes aegypti
Wolbachia
wMel strain
Desiccation resistance
Embryogenesis
Egg viability
title_short Embryonic development and egg viability of wMel-infected Aedes aegypti
title_full Embryonic development and egg viability of wMel-infected Aedes aegypti
title_fullStr Embryonic development and egg viability of wMel-infected Aedes aegypti
title_full_unstemmed Embryonic development and egg viability of wMel-infected Aedes aegypti
title_sort Embryonic development and egg viability of wMel-infected Aedes aegypti
author Farnesi, Luana Cristina
author_facet Farnesi, Luana Cristina
Belinato, Thiago Affonso
Gesto, João Silveira Moledo
Martins, Ademir Jesus
Bruno, Rafaela Vieira
Moreira, Luciano Andrade
author_role author
author2 Belinato, Thiago Affonso
Gesto, João Silveira Moledo
Martins, Ademir Jesus
Bruno, Rafaela Vieira
Moreira, Luciano Andrade
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Farnesi, Luana Cristina
Belinato, Thiago Affonso
Gesto, João Silveira Moledo
Martins, Ademir Jesus
Bruno, Rafaela Vieira
Moreira, Luciano Andrade
dc.subject.other.pt_BR.fl_str_mv Aedes aegypti
Wolbachia
Estirpe de wMel
Resistência à dessecação
Embriogênese
Viabilidade do ovo
topic Aedes aegypti
Wolbachia
Estirpe de wMel
Resistência à dessecação
Embriogênese
Viabilidade do ovo
Aedes aegypti
Wolbachia
wMel strain
Desiccation resistance
Embryogenesis
Egg viability
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
Wolbachia
wMel strain
Desiccation resistance
Embryogenesis
Egg viability
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-02T16:34:04Z
dc.date.available.fl_str_mv 2019-09-02T16:34:04Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARNESI, Luana Cristina et al. Embryonic development and egg viability of wMel-infected Aedes aegypti. Parasites Vectors, v. 12, n. 211, p. 1-9, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35256
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-019-3474-z
identifier_str_mv FARNESI, Luana Cristina et al. Embryonic development and egg viability of wMel-infected Aedes aegypti. Parasites Vectors, v. 12, n. 211, p. 1-9, 2019.
1756-3305
10.1186/s13071-019-3474-z
url https://www.arca.fiocruz.br/handle/icict/35256
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/35256/2/LuanaFarnesi_AdemirMartins_etal_IOC_2019.pdf
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