The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing

Detalhes bibliográficos
Autor(a) principal: Farnesi, Luana Cristina
Data de Publicação: 2021
Outros Autores: Carvalho, Fabiano Duarte, Lacerda, Anna Paula Canuto, Moreira, Luciano Andrade, Bruno, Rafaela Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51134
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brazil.
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spelling Farnesi, Luana CristinaCarvalho, Fabiano DuarteLacerda, Anna Paula CanutoMoreira, Luciano AndradeBruno, Rafaela Vieira2022-02-10T14:55:29Z2022-02-10T14:55:29Z2021FARNESI, Luana Cristina et al. The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing. Parasit Vectors, v. 14, n. 1, 21, 2021 . doi: 10.1186/s13071-020-04465-9.1756-3305https://www.arca.fiocruz.br/handle/icict/51134engBioMed CentralThe influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Mosquitos Vetores: Endossimbiontes e Interação Patógeno-Vetor. Belo Horizonte, MG, Brazil./Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brazil.Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brazil./Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brazil.Background: Aedes aegypti control programs have failed to restrain mosquito population expansion and, consequently, the spread of diseases such as dengue, Zika, and Chikungunya. Wolbachia infection of mosquitoes is a new and promising complementary tool for the control of arbovirus transmission. The use of Wolbachia-infected mosquitoes, mass reared using human blood, is currently being tested in several countries. However, the use of human blood for mass rearing mosquitoes, and thus expansion of this strategy, is problematic. With the aim of overcoming this problem, we tested the effect of different types of blood source on the fitness parameters of female Ae. aegypti and the Wolbachia titer over generations to be able to guarantee the suitability of an alternative source to human blood for mass rearing Wolbachia-infected mosquitoes. Methods: We investigated and compared essential parameters of the vector capacity of laboratory strains of Ae. aegypti with and without Wolbachia that fed on blood of different types of host (human, guinea pig, and mouse). The parameters analyzed were fecundity, fertility, pupation dynamics, and adult survival. Also, we tested whether it is possible to maintain mosquitoes with Wolbachia on mouse blood over generations without losing the bacterium titer. Results: The average number of eggs per female, egg viability and pupation dynamics in the Wolbachia-infected mosquito (wMelBr) strain were similar, regardless of the blood source. The F1 progenies of females that fed on mouse blood or human blood were analyzed. The longevity of males was lower than that of females. F1 female survival differed depending on the presence of Wolbachia in the mother. In subsequent generations analyzed up until F35, the relative Wolbachia density was even higher when mosquitoes fed on mouse blood in comparison to human blood. Conclusions: Taken together, our results provide no evidence that the different types of blood influenced the fitness of the Wolbachia-infected mosquitoes. The presence of the bacterium in the colonies of Wolbachia-infected Ae. aegypti after 35 generations under the conditions evaluated indicates that they can be maintained on mouse blood. Based on these results, we show that it is possible to use mouse blood to feed female mosquitoes when using human blood for this purpose is problematic.Aedes aegyptiBlood feedingVector capacityWolbachiawMel straininfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51134/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALThe influence of different sources of blood.pdfThe influence of different sources of blood.pdfapplication/pdf5794044https://www.arca.fiocruz.br/bitstream/icict/51134/2/The%20influence%20of%20different%20sources%20of%20blood.pdf2ead235fd1a50882446f2ef7df1405c3MD52icict/511342022-02-10 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dc.title.pt_BR.fl_str_mv The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing
title The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing
spellingShingle The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing
Farnesi, Luana Cristina
Aedes aegypti
Blood feeding
Vector capacity
Wolbachia
wMel strain
title_short The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing
title_full The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing
title_fullStr The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing
title_full_unstemmed The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing
title_sort The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing
author Farnesi, Luana Cristina
author_facet Farnesi, Luana Cristina
Carvalho, Fabiano Duarte
Lacerda, Anna Paula Canuto
Moreira, Luciano Andrade
Bruno, Rafaela Vieira
author_role author
author2 Carvalho, Fabiano Duarte
Lacerda, Anna Paula Canuto
Moreira, Luciano Andrade
Bruno, Rafaela Vieira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Farnesi, Luana Cristina
Carvalho, Fabiano Duarte
Lacerda, Anna Paula Canuto
Moreira, Luciano Andrade
Bruno, Rafaela Vieira
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
Blood feeding
Vector capacity
Wolbachia
wMel strain
topic Aedes aegypti
Blood feeding
Vector capacity
Wolbachia
wMel strain
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brazil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-02-10T14:55:29Z
dc.date.available.fl_str_mv 2022-02-10T14:55:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARNESI, Luana Cristina et al. The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing. Parasit Vectors, v. 14, n. 1, 21, 2021 . doi: 10.1186/s13071-020-04465-9.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51134
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
identifier_str_mv FARNESI, Luana Cristina et al. The influence of different sources of blood meals on the physiology of Aedes aegypti harboring Wolbachia wMel: mouse blood as an alternative for mosquito rearing. Parasit Vectors, v. 14, n. 1, 21, 2021 . doi: 10.1186/s13071-020-04465-9.
1756-3305
url https://www.arca.fiocruz.br/handle/icict/51134
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51134/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51134/2/The%20influence%20of%20different%20sources%20of%20blood.pdf
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