Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library

Detalhes bibliográficos
Autor(a) principal: Galvão, Cleber Eduardo
Data de Publicação: 2017
Outros Autores: Fragoso, Stenio Perdigão, Oliveira, Carina Elisei de, Forner, Odinéia, Pereira, Renata Ribeiro Bastos, Soares, Cleber Oliveira, Rosinha, Grácia Maria Soares
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28690
Resumo: Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil.
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spelling Galvão, Cleber EduardoFragoso, Stenio PerdigãoOliveira, Carina Elisei deForner, OdinéiaPereira, Renata Ribeiro BastosSoares, Cleber OliveiraRosinha, Grácia Maria Soares2018-09-12T15:14:52Z2018-09-12T15:14:52Z2017GALVÃO, Cleber Eduardo et al. Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. BMC Microbiology, v. 17, n. 202, 2017.1471-2180https://www.arca.fiocruz.br/handle/icict/2869010.1186/s12866-017-1110-71471-2180porSpringer NatureCorynebacterium pseudotuberculosisLymphadenitisGene ExpressionGene LibraryVaccinesLinfadenitisExpresión GénicaBiblioteca de GenesVacunas de ADNLinfadeniteExpressão GênicaBiblioteca GênicaVacinas de DNAIdentification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression libraryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Católica Dom Bosco. Campo Grande, MS, Brasil.Universidade Federal do Paraná. Curitiba, PR, Brasil.Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil.Embrapa Gado de Corte. Saúde Animal. Laboratório de Engenharia Genética Animal, Campo Grande, MS, Brasil.Embrapa Gado de Corte. Saúde Animal. Laboratório de Engenharia Genética Animal, Campo Grande, MS, Brasil.Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. For this, a wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli. For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. This way, concluded that, proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/28690/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALBMCMicrobiol_1110.pdfBMCMicrobiol_1110.pdfapplication/pdf505969https://www.arca.fiocruz.br/bitstream/icict/28690/2/BMCMicrobiol_1110.pdfcbfec29cc2b04e206a5363e233231737MD52TEXTBMCMicrobiol_1110.pdf.txtBMCMicrobiol_1110.pdf.txtExtracted texttext/plain41166https://www.arca.fiocruz.br/bitstream/icict/28690/3/BMCMicrobiol_1110.pdf.txt13635ec1feebb531952472492cf7ccbdMD53icict/286902018-09-13 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dc.title.pt_BR.fl_str_mv Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library
title Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library
spellingShingle Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library
Galvão, Cleber Eduardo
Corynebacterium pseudotuberculosis
Lymphadenitis
Gene Expression
Gene Library
Vaccines
Linfadenitis
Expresión Génica
Biblioteca de Genes
Vacunas de ADN
Linfadenite
Expressão Gênica
Biblioteca Gênica
Vacinas de DNA
title_short Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library
title_full Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library
title_fullStr Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library
title_full_unstemmed Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library
title_sort Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library
author Galvão, Cleber Eduardo
author_facet Galvão, Cleber Eduardo
Fragoso, Stenio Perdigão
Oliveira, Carina Elisei de
Forner, Odinéia
Pereira, Renata Ribeiro Bastos
Soares, Cleber Oliveira
Rosinha, Grácia Maria Soares
author_role author
author2 Fragoso, Stenio Perdigão
Oliveira, Carina Elisei de
Forner, Odinéia
Pereira, Renata Ribeiro Bastos
Soares, Cleber Oliveira
Rosinha, Grácia Maria Soares
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Galvão, Cleber Eduardo
Fragoso, Stenio Perdigão
Oliveira, Carina Elisei de
Forner, Odinéia
Pereira, Renata Ribeiro Bastos
Soares, Cleber Oliveira
Rosinha, Grácia Maria Soares
dc.subject.other.pt_BR.fl_str_mv Corynebacterium pseudotuberculosis
topic Corynebacterium pseudotuberculosis
Lymphadenitis
Gene Expression
Gene Library
Vaccines
Linfadenitis
Expresión Génica
Biblioteca de Genes
Vacunas de ADN
Linfadenite
Expressão Gênica
Biblioteca Gênica
Vacinas de DNA
dc.subject.en.pt_BR.fl_str_mv Lymphadenitis
Gene Expression
Gene Library
Vaccines
dc.subject.es.pt_BR.fl_str_mv Linfadenitis
Expresión Génica
Biblioteca de Genes
Vacunas de ADN
dc.subject.decs.pt_BR.fl_str_mv Linfadenite
Expressão Gênica
Biblioteca Gênica
Vacinas de DNA
description Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-09-12T15:14:52Z
dc.date.available.fl_str_mv 2018-09-12T15:14:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GALVÃO, Cleber Eduardo et al. Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. BMC Microbiology, v. 17, n. 202, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28690
dc.identifier.issn.pt_BR.fl_str_mv 1471-2180
dc.identifier.doi.none.fl_str_mv 10.1186/s12866-017-1110-7
dc.identifier.eissn.none.fl_str_mv 1471-2180
identifier_str_mv GALVÃO, Cleber Eduardo et al. Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. BMC Microbiology, v. 17, n. 202, 2017.
1471-2180
10.1186/s12866-017-1110-7
url https://www.arca.fiocruz.br/handle/icict/28690
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/28690/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/28690/2/BMCMicrobiol_1110.pdf
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