Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28690 |
Resumo: | Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil. |
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Galvão, Cleber EduardoFragoso, Stenio PerdigãoOliveira, Carina Elisei deForner, OdinéiaPereira, Renata Ribeiro BastosSoares, Cleber OliveiraRosinha, Grácia Maria Soares2018-09-12T15:14:52Z2018-09-12T15:14:52Z2017GALVÃO, Cleber Eduardo et al. Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. BMC Microbiology, v. 17, n. 202, 2017.1471-2180https://www.arca.fiocruz.br/handle/icict/2869010.1186/s12866-017-1110-71471-2180porSpringer NatureCorynebacterium pseudotuberculosisLymphadenitisGene ExpressionGene LibraryVaccinesLinfadenitisExpresión GénicaBiblioteca de GenesVacunas de ADNLinfadeniteExpressão GênicaBiblioteca GênicaVacinas de DNAIdentification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression libraryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Católica Dom Bosco. Campo Grande, MS, Brasil.Universidade Federal do Paraná. Curitiba, PR, Brasil.Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil.Embrapa Gado de Corte. Saúde Animal. Laboratório de Engenharia Genética Animal, Campo Grande, MS, Brasil.Embrapa Gado de Corte. Saúde Animal. Laboratório de Engenharia Genética Animal, Campo Grande, MS, Brasil.Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. For this, a wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA. Sequences between 1000 and 5000 base pairs (bp) were excised from the gel, purified, and the digested DNA fragments were joined to bacteriophage vector ZAP Express, packaged into phage and transfected into Escherichia coli. For immunoscreening a positive sheep sera pool and a negative sera pool for CLA were used. Four clones were identified that strongly reacted to sera. The clones were confirmed by polymerase chain reaction (PCR) followed by sequencing for genomic comparison of C. pseudotuberculosis in GenBank. The genes identified were dak2, fagA, fagB, NlpC/P60 protein family and LPxTG putative protein family. This way, concluded that, proteins of this type can be antigenic which could aid in the development of subunit or DNA vaccines against CLA as well as in the development of serological tests for diagnosis. Immunoscreening of the gene expression library was shown to be a sensitive and efficient technique to identify probable immunodominant genes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/28690/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALBMCMicrobiol_1110.pdfBMCMicrobiol_1110.pdfapplication/pdf505969https://www.arca.fiocruz.br/bitstream/icict/28690/2/BMCMicrobiol_1110.pdfcbfec29cc2b04e206a5363e233231737MD52TEXTBMCMicrobiol_1110.pdf.txtBMCMicrobiol_1110.pdf.txtExtracted texttext/plain41166https://www.arca.fiocruz.br/bitstream/icict/28690/3/BMCMicrobiol_1110.pdf.txt13635ec1feebb531952472492cf7ccbdMD53icict/286902018-09-13 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dc.title.pt_BR.fl_str_mv |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library |
title |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library |
spellingShingle |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library Galvão, Cleber Eduardo Corynebacterium pseudotuberculosis Lymphadenitis Gene Expression Gene Library Vaccines Linfadenitis Expresión Génica Biblioteca de Genes Vacunas de ADN Linfadenite Expressão Gênica Biblioteca Gênica Vacinas de DNA |
title_short |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library |
title_full |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library |
title_fullStr |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library |
title_full_unstemmed |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library |
title_sort |
Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library |
author |
Galvão, Cleber Eduardo |
author_facet |
Galvão, Cleber Eduardo Fragoso, Stenio Perdigão Oliveira, Carina Elisei de Forner, Odinéia Pereira, Renata Ribeiro Bastos Soares, Cleber Oliveira Rosinha, Grácia Maria Soares |
author_role |
author |
author2 |
Fragoso, Stenio Perdigão Oliveira, Carina Elisei de Forner, Odinéia Pereira, Renata Ribeiro Bastos Soares, Cleber Oliveira Rosinha, Grácia Maria Soares |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Galvão, Cleber Eduardo Fragoso, Stenio Perdigão Oliveira, Carina Elisei de Forner, Odinéia Pereira, Renata Ribeiro Bastos Soares, Cleber Oliveira Rosinha, Grácia Maria Soares |
dc.subject.other.pt_BR.fl_str_mv |
Corynebacterium pseudotuberculosis |
topic |
Corynebacterium pseudotuberculosis Lymphadenitis Gene Expression Gene Library Vaccines Linfadenitis Expresión Génica Biblioteca de Genes Vacunas de ADN Linfadenite Expressão Gênica Biblioteca Gênica Vacinas de DNA |
dc.subject.en.pt_BR.fl_str_mv |
Lymphadenitis Gene Expression Gene Library Vaccines |
dc.subject.es.pt_BR.fl_str_mv |
Linfadenitis Expresión Génica Biblioteca de Genes Vacunas de ADN |
dc.subject.decs.pt_BR.fl_str_mv |
Linfadenite Expressão Gênica Biblioteca Gênica Vacinas de DNA |
description |
Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-09-12T15:14:52Z |
dc.date.available.fl_str_mv |
2018-09-12T15:14:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GALVÃO, Cleber Eduardo et al. Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. BMC Microbiology, v. 17, n. 202, 2017. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/28690 |
dc.identifier.issn.pt_BR.fl_str_mv |
1471-2180 |
dc.identifier.doi.none.fl_str_mv |
10.1186/s12866-017-1110-7 |
dc.identifier.eissn.none.fl_str_mv |
1471-2180 |
identifier_str_mv |
GALVÃO, Cleber Eduardo et al. Identification of new Corynebacterium pseudotuberculosis antigens by immunoscreening of gene expression library. BMC Microbiology, v. 17, n. 202, 2017. 1471-2180 10.1186/s12866-017-1110-7 |
url |
https://www.arca.fiocruz.br/handle/icict/28690 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Springer Nature |
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Springer Nature |
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