Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection.

Detalhes bibliográficos
Autor(a) principal: Weinkopff, Tiffany
Data de Publicação: 2013
Outros Autores: Mariotto, Anita, Simon, Gregoire, Torre, Yazmin Hauyon-La, Auderset, Floriane, Schuster, Steffen, Zangger, Haroun, Fasel, Nicolas, Barral, Aldina Maria Prado, Cottiera, Fabienne Tacchini
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8718
Resumo: Department of Biochemistrya and WHO-Immunology Research and Training Center / University of Lausanne. Epalinges, Switzerland
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spelling Weinkopff, TiffanyMariotto, AnitaSimon, GregoireTorre, Yazmin Hauyon-LaAuderset, FlorianeSchuster, SteffenZangger, HarounFasel, NicolasBarral, Aldina Maria PradoCottiera, Fabienne Tacchini2014-11-04T19:29:19Z2014-11-04T19:29:19Z2013WEINKOPFF, T. et al. Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection. Infection and Immunity, v. 81, n. 5, p. 1575-1584, 2013.1098-5522https://www.arca.fiocruz.br/handle/icict/871810.1128/IAI.01401-12porAmerican Society for MicrobiologyRole of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDepartment of Biochemistrya and WHO-Immunology Research and Training Center / University of Lausanne. Epalinges, SwitzerlandDepartment of Biochemistrya and WHO-Immunology Research and Training Center / University of Lausanne. Epalinges, SwitzerlandDepartment of Biochemistrya and WHO-Immunology Research and Training Center / University of Lausanne. Epalinges, SwitzerlandDepartment of Biochemistrya and WHO-Immunology Research and Training Center / University of Lausanne. Epalinges, SwitzerlandDepartment of Biochemistrya and WHO-Immunology Research and Training Center / University of Lausanne. Epalinges, SwitzerlandDepartment of Biochemistrya and WHO-Immunology Research and Training Center / University of Lausanne. Epalinges, SwitzerlandDepartment of Biochemistrya and WHO-Immunology Research and Training CenterDepartment of Biochemistrya and WHO-Immunology Research and Training CenterFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilDepartment of Biochemistrya and WHO-Immunology Research and Training Center / University of Lausanne. Epalinges, SwitzerlandInfection with Leishmania braziliensis causes cutaneous or mucocutaneous leishmaniasis in humans. Toll-like receptor 9 (TLR9) expression has been found in granulomas of lesions in L. braziliensis-infected individuals. L. braziliensis inoculation in mice induces very small lesions that are self-healing, whereas deficiency in the TLR adaptor molecule, MyD88, renders mice susceptible to infection. The TLR involved has not been identified, prompting us to investigate if TLR9 triggering by the parasite contributes to the strong resistance to infection observed in L. braziliensis-inoculated mice. The parasites activated wild-type (WT) dendritic cells (DCs) in vitro but not DCs derived from TLR9(-/-) mice. TLR9(-/-) mice inoculated with L. braziliensis exhibited a transient susceptibility characterized by increased lesion size and parasite burden compared to those of WT mice. Surprisingly, elevated levels of gamma interferon (IFN-γ) were measured at the site of infection and in draining lymph node T cells of TLR9(-/-) mice at the peak of susceptibility, suggesting that unlike observations in vitro, the parasite could induce DC activation leading to the development of Th1 cells in the absence of TLR9 expression. Taken together, these data show that TLR9 signaling is important for the early control of lesion development and parasite burden but is dispensable for the differentiation of Th1 cells secreting IFN-γ, and the high levels of this cytokine are not sufficient to control early parasite replication following L. braziliensis infectionLeishmania braziliensisLeishmaniose Cutânea/metabolismoReceptor Toll-Like 9/fisiologiaAnimaisCitocinas/metabolismoModelos Animais de DoençasFemininoLeishmaniose Cutânea/imunologiaMacrófagos/parasitologiaCamundongosCamundongos Endogâmicos C57BLCélulas Th1/imunologiaReceptor Toll-Like 9/deficiênciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8718/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALWeinkopff T Role of TLR9....pdfWeinkopff T Role of TLR9....pdfapplication/pdf1483928https://www.arca.fiocruz.br/bitstream/icict/8718/2/Weinkopff%20T%20Role%20of%20TLR9....pdf0c938f1ddb7d861127ecc557145972d0MD52TEXTWeinkopff T Role of TLR9....pdf.txtWeinkopff T Role of TLR9....pdf.txtExtracted texttext/plain54764https://www.arca.fiocruz.br/bitstream/icict/8718/3/Weinkopff%20T%20Role%20of%20TLR9....pdf.txt493dc741c9890ce3b6e3e9b5c91f8587MD53icict/87182023-03-15 14:33:56.452oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:56Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection.
title Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection.
spellingShingle Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection.
Weinkopff, Tiffany
Leishmania braziliensis
Leishmaniose Cutânea/metabolismo
Receptor Toll-Like 9/fisiologia
Animais
Citocinas/metabolismo
Modelos Animais de Doenças
Feminino
Leishmaniose Cutânea/imunologia
Macrófagos/parasitologia
Camundongos
Camundongos Endogâmicos C57BL
Células Th1/imunologia
Receptor Toll-Like 9/deficiência
title_short Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection.
title_full Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection.
title_fullStr Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection.
title_full_unstemmed Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection.
title_sort Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection.
author Weinkopff, Tiffany
author_facet Weinkopff, Tiffany
Mariotto, Anita
Simon, Gregoire
Torre, Yazmin Hauyon-La
Auderset, Floriane
Schuster, Steffen
Zangger, Haroun
Fasel, Nicolas
Barral, Aldina Maria Prado
Cottiera, Fabienne Tacchini
author_role author
author2 Mariotto, Anita
Simon, Gregoire
Torre, Yazmin Hauyon-La
Auderset, Floriane
Schuster, Steffen
Zangger, Haroun
Fasel, Nicolas
Barral, Aldina Maria Prado
Cottiera, Fabienne Tacchini
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Weinkopff, Tiffany
Mariotto, Anita
Simon, Gregoire
Torre, Yazmin Hauyon-La
Auderset, Floriane
Schuster, Steffen
Zangger, Haroun
Fasel, Nicolas
Barral, Aldina Maria Prado
Cottiera, Fabienne Tacchini
dc.subject.decs.pt_BR.fl_str_mv Leishmania braziliensis
Leishmaniose Cutânea/metabolismo
Receptor Toll-Like 9/fisiologia
Animais
Citocinas/metabolismo
Modelos Animais de Doenças
Feminino
Leishmaniose Cutânea/imunologia
Macrófagos/parasitologia
Camundongos
Camundongos Endogâmicos C57BL
Células Th1/imunologia
Receptor Toll-Like 9/deficiência
topic Leishmania braziliensis
Leishmaniose Cutânea/metabolismo
Receptor Toll-Like 9/fisiologia
Animais
Citocinas/metabolismo
Modelos Animais de Doenças
Feminino
Leishmaniose Cutânea/imunologia
Macrófagos/parasitologia
Camundongos
Camundongos Endogâmicos C57BL
Células Th1/imunologia
Receptor Toll-Like 9/deficiência
description Department of Biochemistrya and WHO-Immunology Research and Training Center / University of Lausanne. Epalinges, Switzerland
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-11-04T19:29:19Z
dc.date.available.fl_str_mv 2014-11-04T19:29:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv WEINKOPFF, T. et al. Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection. Infection and Immunity, v. 81, n. 5, p. 1575-1584, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8718
dc.identifier.issn.none.fl_str_mv 1098-5522
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.01401-12
identifier_str_mv WEINKOPFF, T. et al. Role of Toll-like receptor 9 signaling in experimental Leishmania braziliensis infection. Infection and Immunity, v. 81, n. 5, p. 1575-1584, 2013.
1098-5522
10.1128/IAI.01401-12
url https://www.arca.fiocruz.br/handle/icict/8718
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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