Naïve donor responses to Schistosoma mansoni soluble egg antigens.

Detalhes bibliográficos
Autor(a) principal: Reis, Eliana Almeida Gomes
Data de Publicação: 2007
Outros Autores: Carmo, Theomira Mauadie de Azevedo, McBride, Alan John Alexander, Harn Junior, Donald A, Reis, Mitermayer Galvão dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7545
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Reis, Eliana Almeida GomesCarmo, Theomira Mauadie de AzevedoMcBride, Alan John AlexanderHarn Junior, Donald AReis, Mitermayer Galvão dos2014-04-25T18:33:00Z2014-04-25T18:33:00Z2007REIS, E. A. G. et al. Naïve donor responses to Schistosoma mansoni soluble egg antigens. Scandinavian Journal of Immunology , v.66, n. 6, p. 662–670, 2007.1365-3083https://www.arca.fiocruz.br/handle/icict/754510.1111/j.1365-3083.2007.02024.xengBlackwell Publishing Ltd.Naïve donor responses to Schistosoma mansoni soluble egg antigens.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilDepartment of Immunology and Infectious Diseases. Harvard School of Public Health. Boston, MA, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Faculty of Medicine of Federal University of Bahia. Department of Anatomical Pathology and Legal Medicine. Salvador, BA, BrasilSchistosome infection induces profound Th-biasing and immune suppression. Although much has been examined in mice, few studies have examined responses of naı¨ve humans to schistosome antigens. In this study, we examined the response of naı¨ve human peripheral blood mononuclear cells (nPBMC) to stimulation with Schistosoma mansoni soluble egg antigen (SEA) using a priming in vitro (PIV) assay. We found that SEA induced a pronounced CD4+ T-helper cell response based on cytokine secretion and phenotyping markers. SEA-stimulated nPBMC (SEA cells) at day 7 post-priming and after the first recall consisted predominantly of Th0-like CD4+ T cells. Following the second recall, the majority of donor (10 ⁄ 12) responses were Th2-like. The cell population consisted of approximately 64% CD4+, 17% CD8+high, 12% CD19+, and 7% CD23+ cells. The CD4+ population also expressed HLA-DR+, CD54+, CD45RO+ and CD25+ whereas the CD19+ cells expressed CD80 and CD86. Following priming, we detected high levels of IL-6, IFN-c, IL-12p40, IL-10 and IL-5. Upon restimulation, SEA cells secreted IL-5 and high levels of IL-10, typical of a Th2-like response. The data presented herein shows that the majority of naı¨ve donor dendritic cells, following stimulation with SEA, prime and clonally expand SEA-specific T cells towards a Th2-type response. However, two donors responded with an atypical response, producing IFN-c coincident with low levels of IL-10. Whether this differential response was due to HLA or other genes was not determined but is currently under investigation.Antígenos de Helmintos/administração & dosagemÓvulo/imunologiaSchistosoma mansoni/imunologiaEsquistossomose mansoni/imunologiaAdultoAnimaisAntígenos de Helmintos/imunologiaLinfócitos T CD4-Positivos/imunologiaCitocinas/imunologiaCélulas Dendríticas/parasitologiaCitometria de FluxoHumanosSolubilidadeCélulas Th2/imunologiaCélulas Th2/parasitologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALReis EA Naive Donor....pdfReis EA Naive Donor....pdfapplication/pdf430957https://www.arca.fiocruz.br/bitstream/icict/7545/1/Reis%20EA%20Naive%20Donor....pdf195dd9ec4cd69f51ee6f6008a1a4fa5aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7545/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTReis EA Naive Donor....pdf.txtReis EA Naive Donor....pdf.txtExtracted texttext/plain39370https://www.arca.fiocruz.br/bitstream/icict/7545/5/Reis%20EA%20Naive%20Donor....pdf.txtd4a66053931f4f0c339d4befcf87dbf5MD55THUMBNAILReis EA Naive Donor....pdf.jpgReis EA Naive Donor....pdf.jpgGenerated Thumbnailimage/jpeg2429https://www.arca.fiocruz.br/bitstream/icict/7545/4/Reis%20EA%20Naive%20Donor....pdf.jpg4acac206927aff04d99a84dda26b1b39MD54icict/75452023-03-15 14:33:17.235oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Naïve donor responses to Schistosoma mansoni soluble egg antigens.
title Naïve donor responses to Schistosoma mansoni soluble egg antigens.
spellingShingle Naïve donor responses to Schistosoma mansoni soluble egg antigens.
Reis, Eliana Almeida Gomes
Antígenos de Helmintos/administração & dosagem
Óvulo/imunologia
Schistosoma mansoni/imunologia
Esquistossomose mansoni/imunologia
Adulto
Animais
Antígenos de Helmintos/imunologia
Linfócitos T CD4-Positivos/imunologia
Citocinas/imunologia
Células Dendríticas/parasitologia
Citometria de Fluxo
Humanos
Solubilidade
Células Th2/imunologia
Células Th2/parasitologia
title_short Naïve donor responses to Schistosoma mansoni soluble egg antigens.
title_full Naïve donor responses to Schistosoma mansoni soluble egg antigens.
title_fullStr Naïve donor responses to Schistosoma mansoni soluble egg antigens.
title_full_unstemmed Naïve donor responses to Schistosoma mansoni soluble egg antigens.
title_sort Naïve donor responses to Schistosoma mansoni soluble egg antigens.
author Reis, Eliana Almeida Gomes
author_facet Reis, Eliana Almeida Gomes
Carmo, Theomira Mauadie de Azevedo
McBride, Alan John Alexander
Harn Junior, Donald A
Reis, Mitermayer Galvão dos
author_role author
author2 Carmo, Theomira Mauadie de Azevedo
McBride, Alan John Alexander
Harn Junior, Donald A
Reis, Mitermayer Galvão dos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Reis, Eliana Almeida Gomes
Carmo, Theomira Mauadie de Azevedo
McBride, Alan John Alexander
Harn Junior, Donald A
Reis, Mitermayer Galvão dos
dc.subject.decs.pt_BR.fl_str_mv Antígenos de Helmintos/administração & dosagem
Óvulo/imunologia
Schistosoma mansoni/imunologia
Esquistossomose mansoni/imunologia
Adulto
Animais
Antígenos de Helmintos/imunologia
Linfócitos T CD4-Positivos/imunologia
Citocinas/imunologia
Células Dendríticas/parasitologia
Citometria de Fluxo
Humanos
Solubilidade
Células Th2/imunologia
Células Th2/parasitologia
topic Antígenos de Helmintos/administração & dosagem
Óvulo/imunologia
Schistosoma mansoni/imunologia
Esquistossomose mansoni/imunologia
Adulto
Animais
Antígenos de Helmintos/imunologia
Linfócitos T CD4-Positivos/imunologia
Citocinas/imunologia
Células Dendríticas/parasitologia
Citometria de Fluxo
Humanos
Solubilidade
Células Th2/imunologia
Células Th2/parasitologia
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2014-04-25T18:33:00Z
dc.date.available.fl_str_mv 2014-04-25T18:33:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REIS, E. A. G. et al. Naïve donor responses to Schistosoma mansoni soluble egg antigens. Scandinavian Journal of Immunology , v.66, n. 6, p. 662–670, 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7545
dc.identifier.issn.none.fl_str_mv 1365-3083
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-3083.2007.02024.x
identifier_str_mv REIS, E. A. G. et al. Naïve donor responses to Schistosoma mansoni soluble egg antigens. Scandinavian Journal of Immunology , v.66, n. 6, p. 662–670, 2007.
1365-3083
10.1111/j.1365-3083.2007.02024.x
url https://www.arca.fiocruz.br/handle/icict/7545
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Publishing Ltd.
publisher.none.fl_str_mv Blackwell Publishing Ltd.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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