Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses

Detalhes bibliográficos
Autor(a) principal: Brodskyn, Claudia Ida
Data de Publicação: 2002
Outros Autores: Patricio, Julie, Oliveira, Rubem, Lobo, Lucas, Arnholdt, Andrea, Previato, Lucia Mendonça, Barral, Aldina Maria Prado, Barral Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/4588
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Brodskyn, Claudia IdaPatricio, JulieOliveira, RubemLobo, LucasArnholdt, AndreaPreviato, Lucia MendonçaBarral, Aldina Maria PradoBarral Netto, Manoel2012-09-05T19:38:13Z2012-09-05T19:38:13Z2002BRODSKYN, C. I. et al. Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses. Infection and Immunity, v. 70, n. 7, p. 3736-43, July 2002.0019-9567https://www.arca.fiocruz.br/handle/icict/458810.1128/IAI.70.7.3736–3743.2002engGlycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responsesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade do Norte Fluminense. Laboratório de Biotecnologia. Campos, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilTo investigate the possible effects of glycoinositolphospholipid (GIPL) from Trypanosoma cruzi on human antigen presenting cells, we tested their effects on lipopolysaccharide (LPS)-stimulated human macrophages and dendritic cells (DC). Human macrophages or DC were incubated with GIPL (50 microg/ml) and LPS (500 pg/ml) and tumor necrosis factor alpha (TNF-alpha), interleukin 8 (IL-8), IL-10, and IL-12p40 levels in supernatants were analyzed by enzyme-linked immunosorbent assay. TNF-alpha, IL-10, and IL-12 secretion were significantly decreased by GIPL both in macrophages and DC. In contrast, GIPL did not alter IL-8 production. We also analyzed the expression of CD80, CD86, HLA-DR, CD40, and CD57 on the macrophage surface after stimulation with LPS in the presence or absence of T. cruzi GIPL. GIPL led to a down-regulation in the expression of all tested molecules. We additionally examined the influence of T. cruzi GIPL on the response of human DC to LPS. LPS-induced HLA-DR, CD83, and CD86 up-regulation was significantly inhibited by GIPL. A slight down-regulation in CD80 and CD40 expression on DC surfaces in the presence of GIPL was also noticed. Similarly, GIPL led to down-modulation of CD83, CD80, CD86, and HLA-DR surface expression and TNF-alpha and IL-10 production when DC were stimulated by CD40L. The ceramide portion of GIPL was responsible for most of the activity exhibited by the whole molecule. Considering the important role of the immune response in determining the fate of the host-parasite relationship, the immunoregulatory activities of T. cruzi GIPL are potentially important for parasite evasion and then pathogenesis of infection with protozoan parasites.Células Dendríticas/imunologiaGlicolipídeos/imunologiaMacrófagos/imunologiaFosfolipídeos/imunologiaTrypanosoma cruzi/imunologiaAntígenos CD/biossínteseAntígenos CD80/biossínteseAntígenos CD86Células CultivadasCeramidas/imunologiaCélulas Dendríticas/citologiaGlicolipídeos/farmacologiaMolécula 1 de Adesão Intercelular/biossínteseInterleucina-10/biossínteseLipopolissacarídeos/farmacologiaFator de Necrose Tumoral alfa/biossínteseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBrodskyn c Glycoinositolphospholipids from....pdfBrodskyn c Glycoinositolphospholipids from....pdfapplication/pdf581144https://www.arca.fiocruz.br/bitstream/icict/4588/1/Brodskyn%20c%20Glycoinositolphospholipids%20from....pdf2f891984ec8159f1c60f6165545db099MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/4588/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTBrodskyn c Glycoinositolphospholipids from....pdf.txtBrodskyn c Glycoinositolphospholipids from....pdf.txtExtracted texttext/plain35283https://www.arca.fiocruz.br/bitstream/icict/4588/5/Brodskyn%20c%20Glycoinositolphospholipids%20from....pdf.txt7ecbf880ba403b361458579fae303b2cMD55THUMBNAILBrodskyn c Glycoinositolphospholipids from....pdf.jpgBrodskyn c Glycoinositolphospholipids from....pdf.jpgGenerated Thumbnailimage/jpeg2354https://www.arca.fiocruz.br/bitstream/icict/4588/4/Brodskyn%20c%20Glycoinositolphospholipids%20from....pdf.jpgac7e968da0da6d4847df5b65a8becce3MD54icict/45882023-03-15 14:33:12.489oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:12Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
title Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
spellingShingle Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
Brodskyn, Claudia Ida
Células Dendríticas/imunologia
Glicolipídeos/imunologia
Macrófagos/imunologia
Fosfolipídeos/imunologia
Trypanosoma cruzi/imunologia
Antígenos CD/biossíntese
Antígenos CD80/biossíntese
Antígenos CD86
Células Cultivadas
Ceramidas/imunologia
Células Dendríticas/citologia
Glicolipídeos/farmacologia
Molécula 1 de Adesão Intercelular/biossíntese
Interleucina-10/biossíntese
Lipopolissacarídeos/farmacologia
Fator de Necrose Tumoral alfa/biossíntese
title_short Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
title_full Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
title_fullStr Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
title_full_unstemmed Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
title_sort Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
author Brodskyn, Claudia Ida
author_facet Brodskyn, Claudia Ida
Patricio, Julie
Oliveira, Rubem
Lobo, Lucas
Arnholdt, Andrea
Previato, Lucia Mendonça
Barral, Aldina Maria Prado
Barral Netto, Manoel
author_role author
author2 Patricio, Julie
Oliveira, Rubem
Lobo, Lucas
Arnholdt, Andrea
Previato, Lucia Mendonça
Barral, Aldina Maria Prado
Barral Netto, Manoel
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Brodskyn, Claudia Ida
Patricio, Julie
Oliveira, Rubem
Lobo, Lucas
Arnholdt, Andrea
Previato, Lucia Mendonça
Barral, Aldina Maria Prado
Barral Netto, Manoel
dc.subject.decs.pt_BR.fl_str_mv Células Dendríticas/imunologia
Glicolipídeos/imunologia
Macrófagos/imunologia
Fosfolipídeos/imunologia
Trypanosoma cruzi/imunologia
Antígenos CD/biossíntese
Antígenos CD80/biossíntese
Antígenos CD86
Células Cultivadas
Ceramidas/imunologia
Células Dendríticas/citologia
Glicolipídeos/farmacologia
Molécula 1 de Adesão Intercelular/biossíntese
Interleucina-10/biossíntese
Lipopolissacarídeos/farmacologia
Fator de Necrose Tumoral alfa/biossíntese
topic Células Dendríticas/imunologia
Glicolipídeos/imunologia
Macrófagos/imunologia
Fosfolipídeos/imunologia
Trypanosoma cruzi/imunologia
Antígenos CD/biossíntese
Antígenos CD80/biossíntese
Antígenos CD86
Células Cultivadas
Ceramidas/imunologia
Células Dendríticas/citologia
Glicolipídeos/farmacologia
Molécula 1 de Adesão Intercelular/biossíntese
Interleucina-10/biossíntese
Lipopolissacarídeos/farmacologia
Fator de Necrose Tumoral alfa/biossíntese
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2012-09-05T19:38:13Z
dc.date.available.fl_str_mv 2012-09-05T19:38:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BRODSKYN, C. I. et al. Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses. Infection and Immunity, v. 70, n. 7, p. 3736-43, July 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/4588
dc.identifier.issn.none.fl_str_mv 0019-9567
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.70.7.3736–3743.2002
identifier_str_mv BRODSKYN, C. I. et al. Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses. Infection and Immunity, v. 70, n. 7, p. 3736-43, July 2002.
0019-9567
10.1128/IAI.70.7.3736–3743.2002
url https://www.arca.fiocruz.br/handle/icict/4588
dc.language.iso.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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