Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/4588 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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Brodskyn, Claudia IdaPatricio, JulieOliveira, RubemLobo, LucasArnholdt, AndreaPreviato, Lucia MendonçaBarral, Aldina Maria PradoBarral Netto, Manoel2012-09-05T19:38:13Z2012-09-05T19:38:13Z2002BRODSKYN, C. I. et al. Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses. Infection and Immunity, v. 70, n. 7, p. 3736-43, July 2002.0019-9567https://www.arca.fiocruz.br/handle/icict/458810.1128/IAI.70.7.3736–3743.2002engGlycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responsesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade do Norte Fluminense. Laboratório de Biotecnologia. Campos, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilTo investigate the possible effects of glycoinositolphospholipid (GIPL) from Trypanosoma cruzi on human antigen presenting cells, we tested their effects on lipopolysaccharide (LPS)-stimulated human macrophages and dendritic cells (DC). Human macrophages or DC were incubated with GIPL (50 microg/ml) and LPS (500 pg/ml) and tumor necrosis factor alpha (TNF-alpha), interleukin 8 (IL-8), IL-10, and IL-12p40 levels in supernatants were analyzed by enzyme-linked immunosorbent assay. TNF-alpha, IL-10, and IL-12 secretion were significantly decreased by GIPL both in macrophages and DC. In contrast, GIPL did not alter IL-8 production. We also analyzed the expression of CD80, CD86, HLA-DR, CD40, and CD57 on the macrophage surface after stimulation with LPS in the presence or absence of T. cruzi GIPL. GIPL led to a down-regulation in the expression of all tested molecules. We additionally examined the influence of T. cruzi GIPL on the response of human DC to LPS. LPS-induced HLA-DR, CD83, and CD86 up-regulation was significantly inhibited by GIPL. A slight down-regulation in CD80 and CD40 expression on DC surfaces in the presence of GIPL was also noticed. Similarly, GIPL led to down-modulation of CD83, CD80, CD86, and HLA-DR surface expression and TNF-alpha and IL-10 production when DC were stimulated by CD40L. The ceramide portion of GIPL was responsible for most of the activity exhibited by the whole molecule. Considering the important role of the immune response in determining the fate of the host-parasite relationship, the immunoregulatory activities of T. cruzi GIPL are potentially important for parasite evasion and then pathogenesis of infection with protozoan parasites.Células Dendríticas/imunologiaGlicolipídeos/imunologiaMacrófagos/imunologiaFosfolipídeos/imunologiaTrypanosoma cruzi/imunologiaAntígenos CD/biossínteseAntígenos CD80/biossínteseAntígenos CD86Células CultivadasCeramidas/imunologiaCélulas Dendríticas/citologiaGlicolipídeos/farmacologiaMolécula 1 de Adesão Intercelular/biossínteseInterleucina-10/biossínteseLipopolissacarídeos/farmacologiaFator de Necrose Tumoral alfa/biossínteseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBrodskyn c Glycoinositolphospholipids from....pdfBrodskyn c Glycoinositolphospholipids from....pdfapplication/pdf581144https://www.arca.fiocruz.br/bitstream/icict/4588/1/Brodskyn%20c%20Glycoinositolphospholipids%20from....pdf2f891984ec8159f1c60f6165545db099MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/4588/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTBrodskyn c Glycoinositolphospholipids from....pdf.txtBrodskyn c Glycoinositolphospholipids from....pdf.txtExtracted texttext/plain35283https://www.arca.fiocruz.br/bitstream/icict/4588/5/Brodskyn%20c%20Glycoinositolphospholipids%20from....pdf.txt7ecbf880ba403b361458579fae303b2cMD55THUMBNAILBrodskyn c Glycoinositolphospholipids from....pdf.jpgBrodskyn c Glycoinositolphospholipids from....pdf.jpgGenerated Thumbnailimage/jpeg2354https://www.arca.fiocruz.br/bitstream/icict/4588/4/Brodskyn%20c%20Glycoinositolphospholipids%20from....pdf.jpgac7e968da0da6d4847df5b65a8becce3MD54icict/45882023-03-15 14:33:12.489oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:12Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses |
title |
Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses |
spellingShingle |
Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses Brodskyn, Claudia Ida Células Dendríticas/imunologia Glicolipídeos/imunologia Macrófagos/imunologia Fosfolipídeos/imunologia Trypanosoma cruzi/imunologia Antígenos CD/biossíntese Antígenos CD80/biossíntese Antígenos CD86 Células Cultivadas Ceramidas/imunologia Células Dendríticas/citologia Glicolipídeos/farmacologia Molécula 1 de Adesão Intercelular/biossíntese Interleucina-10/biossíntese Lipopolissacarídeos/farmacologia Fator de Necrose Tumoral alfa/biossíntese |
title_short |
Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses |
title_full |
Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses |
title_fullStr |
Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses |
title_full_unstemmed |
Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses |
title_sort |
Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses |
author |
Brodskyn, Claudia Ida |
author_facet |
Brodskyn, Claudia Ida Patricio, Julie Oliveira, Rubem Lobo, Lucas Arnholdt, Andrea Previato, Lucia Mendonça Barral, Aldina Maria Prado Barral Netto, Manoel |
author_role |
author |
author2 |
Patricio, Julie Oliveira, Rubem Lobo, Lucas Arnholdt, Andrea Previato, Lucia Mendonça Barral, Aldina Maria Prado Barral Netto, Manoel |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Brodskyn, Claudia Ida Patricio, Julie Oliveira, Rubem Lobo, Lucas Arnholdt, Andrea Previato, Lucia Mendonça Barral, Aldina Maria Prado Barral Netto, Manoel |
dc.subject.decs.pt_BR.fl_str_mv |
Células Dendríticas/imunologia Glicolipídeos/imunologia Macrófagos/imunologia Fosfolipídeos/imunologia Trypanosoma cruzi/imunologia Antígenos CD/biossíntese Antígenos CD80/biossíntese Antígenos CD86 Células Cultivadas Ceramidas/imunologia Células Dendríticas/citologia Glicolipídeos/farmacologia Molécula 1 de Adesão Intercelular/biossíntese Interleucina-10/biossíntese Lipopolissacarídeos/farmacologia Fator de Necrose Tumoral alfa/biossíntese |
topic |
Células Dendríticas/imunologia Glicolipídeos/imunologia Macrófagos/imunologia Fosfolipídeos/imunologia Trypanosoma cruzi/imunologia Antígenos CD/biossíntese Antígenos CD80/biossíntese Antígenos CD86 Células Cultivadas Ceramidas/imunologia Células Dendríticas/citologia Glicolipídeos/farmacologia Molécula 1 de Adesão Intercelular/biossíntese Interleucina-10/biossíntese Lipopolissacarídeos/farmacologia Fator de Necrose Tumoral alfa/biossíntese |
description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2012-09-05T19:38:13Z |
dc.date.available.fl_str_mv |
2012-09-05T19:38:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BRODSKYN, C. I. et al. Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses. Infection and Immunity, v. 70, n. 7, p. 3736-43, July 2002. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/4588 |
dc.identifier.issn.none.fl_str_mv |
0019-9567 |
dc.identifier.doi.none.fl_str_mv |
10.1128/IAI.70.7.3736–3743.2002 |
identifier_str_mv |
BRODSKYN, C. I. et al. Glycoinositolphospholipids from Trypanosoma cruzi interfere with macrophages and dendritic cell responses. Infection and Immunity, v. 70, n. 7, p. 3736-43, July 2002. 0019-9567 10.1128/IAI.70.7.3736–3743.2002 |
url |
https://www.arca.fiocruz.br/handle/icict/4588 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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