Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Nunes, Sara
Data de Publicação: 2023
Outros Autores: Tibúrcio, Rafael, Silva, Icaro Bonyek, Oliveira, Pablo Rafael, Khouri, Ricardo, Boaventura, Viviane, Barral, Aldina, Brodskyn, Cláudia, Tavares, Natalia Machado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/61265
Resumo: Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES ).
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spelling Nunes, SaraTibúrcio, RafaelSilva, Icaro BonyekOliveira, Pablo RafaelKhouri, RicardoBoaventura, VivianeBarral, AldinaBrodskyn, CláudiaTavares, Natalia Machado2023-11-09T19:43:57Z2023-11-09T19:43:57Z2023NUNES, Sara Nunes et al. Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis. Microorganisms, v. 11, n. 8, p. 1-14, 2023.2076-2607https://www.arca.fiocruz.br/handle/icict/6126510.3390/microorganisms11081937Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES ).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil.Instituto Federal Baiano. Xique-Xique, BA, Brasil.Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz, Laboratório de Doenças Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz, Laboratório de Doenças Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz, Laboratório de Doenças Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Biologia. Salvador, BA, Brasil.Skin ulcers of cutaneous leishmaniasis (CL) are characterized by a localized inflammatory response mediated by innate and adaptive immune cells, including dendritic cells (DC) and natural killer (NK) cells. Bidirectional interactions between DCs and NK cells contribute to tailor leishmaniasis outcome. Despite advances in the Leishmania biology field in recent decades, the mechanisms involved in DC/NK-mediated control of Leishmania sp. pathogenesis as well as the cellular and molecular players involved in such interaction remain unclear. The present study sought to investigate canonical pathways associated with CL arising from Leishmania braziliensis infection. Initially, two publicly available microarray datasets of skin biopsies from active CL lesions were analyzed, and five pathways were identified using differentially expressed genes. The “Crosstalk between DCs and NK cells” pathway was notable due to a high number of modulated genes. The molecules significantly involved in this pathway were identified, and our findings were validated in newly obtained CL biopsies. We found increased expression of TLR4, TNFRSF1B, IL-15, IL-6, CD40, CCR7, TNF and IFNG, confirming the analysis of publicly available datasets. These findings reveal the “crosstalk between DCs and NK cells” as a potential pathway to be further explored in the pathogenesis of CL, especially the expression of CCR7, which is correlated with lesion development.engMDPILeishmania braziliensisCélulas assassinas naturaisCélulas dendríticasTranscriptomaLeishmaniose cutâneaLeishmania braziliensisNatural killer cellsDendritic cellsTranscriptomeCutaneous leishmaniasisLeishmania braziliensisCélulas Matadoras NaturaisCélulas DendríticasTranscriptomaLeishmanioseInfecçõesTranscriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/61265/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALNunes, Sara - Transcriptome Analysis Identifies the Crosstalk between Dendritic and Natural Killer.pdfNunes, Sara - Transcriptome Analysis Identifies the Crosstalk between Dendritic and Natural Killer.pdfapplication/pdf3475113https://www.arca.fiocruz.br/bitstream/icict/61265/2/Nunes%2c%20Sara%20-%20Transcriptome%20Analysis%20Identifies%20the%20Crosstalk%20between%20Dendritic%20and%20Natural%20Killer.pdf99bcdf711e6dd71f7598084ac955ed30MD52icict/612652023-11-09 16:43:58.121oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-11-09T19:43:58Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.en_US.fl_str_mv Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis
title Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis
spellingShingle Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis
Nunes, Sara
Leishmania braziliensis
Células assassinas naturais
Células dendríticas
Transcriptoma
Leishmaniose cutânea
Leishmania braziliensis
Natural killer cells
Dendritic cells
Transcriptome
Cutaneous leishmaniasis
Leishmania braziliensis
Células Matadoras Naturais
Células Dendríticas
Transcriptoma
Leishmaniose
Infecções
title_short Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis
title_full Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis
title_fullStr Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis
title_full_unstemmed Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis
title_sort Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis
author Nunes, Sara
author_facet Nunes, Sara
Tibúrcio, Rafael
Silva, Icaro Bonyek
Oliveira, Pablo Rafael
Khouri, Ricardo
Boaventura, Viviane
Barral, Aldina
Brodskyn, Cláudia
Tavares, Natalia Machado
author_role author
author2 Tibúrcio, Rafael
Silva, Icaro Bonyek
Oliveira, Pablo Rafael
Khouri, Ricardo
Boaventura, Viviane
Barral, Aldina
Brodskyn, Cláudia
Tavares, Natalia Machado
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nunes, Sara
Tibúrcio, Rafael
Silva, Icaro Bonyek
Oliveira, Pablo Rafael
Khouri, Ricardo
Boaventura, Viviane
Barral, Aldina
Brodskyn, Cláudia
Tavares, Natalia Machado
dc.subject.other.en_US.fl_str_mv Leishmania braziliensis
Células assassinas naturais
Células dendríticas
Transcriptoma
Leishmaniose cutânea
topic Leishmania braziliensis
Células assassinas naturais
Células dendríticas
Transcriptoma
Leishmaniose cutânea
Leishmania braziliensis
Natural killer cells
Dendritic cells
Transcriptome
Cutaneous leishmaniasis
Leishmania braziliensis
Células Matadoras Naturais
Células Dendríticas
Transcriptoma
Leishmaniose
Infecções
dc.subject.en.en_US.fl_str_mv Leishmania braziliensis
Natural killer cells
Dendritic cells
Transcriptome
Cutaneous leishmaniasis
dc.subject.decs.en_US.fl_str_mv Leishmania braziliensis
Células Matadoras Naturais
Células Dendríticas
Transcriptoma
Leishmaniose
Infecções
description Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES ).
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-11-09T19:43:57Z
dc.date.available.fl_str_mv 2023-11-09T19:43:57Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv NUNES, Sara Nunes et al. Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis. Microorganisms, v. 11, n. 8, p. 1-14, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/61265
dc.identifier.issn.en_US.fl_str_mv 2076-2607
dc.identifier.doi.none.fl_str_mv 10.3390/microorganisms11081937
identifier_str_mv NUNES, Sara Nunes et al. Transcriptome analysis identifies the crosstalk between dendritic and natural killer cells in human cutaneous leishmaniasis. Microorganisms, v. 11, n. 8, p. 1-14, 2023.
2076-2607
10.3390/microorganisms11081937
url https://www.arca.fiocruz.br/handle/icict/61265
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv MDPI
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