Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/27191 |
Resumo: | CNPq/MST/INCT-DT, grant number 465229/2014-0 and National Institutes of Health, grant number: U01 AI136032. |
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Lopes, Diego MotaAlmeida, Tarcísio Vila Verde Santana deSouza, Robson da Paixão deRibeiro, Luís Eduardo VianaPage, BradyFernandes, Jamille de SouzaCarvalho Filho, Edgar MarcelinoCardoso, Luciana Santos2018-06-29T13:48:58Z2018-06-29T13:48:58Z2018LOPES, D. M. et al. Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis. Molecular Immunology, v. 93, p. 173–183, 2018.0161-5890https://www.arca.fiocruz.br/handle/icict/2719110.1016/j.molimm.2017.11.018CNPq/MST/INCT-DT, grant number 465229/2014-0 and National Institutes of Health, grant number: U01 AI136032.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública, Salvador, Bahia, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilTulane University School of Medicine. New Orleans, LA, USAUniversidade Federal da Bahia. Núcleo de Excelência em Asma. Salvador, Bahia, BrazilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilCoinfection with leishmaniasis and schistosomiasis has been associated with increased time to healing of cutaneous lesions of leishmaniasis. The objective of this study was to evaluate the effect of Leishmania braziliensis infection on co-cultures of monocyte-derived dendritic cells (MoDCs) with autologous lymphocytes from patients with schistosomiasis and patients with cutaneous leishmaniasis. MoDCs were differentiated from peripheral blood monocytes, isolated by magnetic beads, infected with L. braziliensis, and co-cultured with autologous lymphocytes. Expression of HLA-DR, CD1a, CD83, CD80, CD86, CD40, and the IL-10 receptor (IL-10R) on MoDCs as well as CD28, CD40L, CD25, and CTLA-4 on lymphocytes were evaluated by flow cytometry. The production of the cytokines IL-10, TNF, IL-12p40, and IFN-γ were evaluated by sandwich ELISA of the culture supernatant. The infectivity evaluation was performed by light microscopy after concentration of cells by cytospin and Giemsa staining. It was observed that the frequency of MoDCs expressing CD83, CD80, and CD86 as well as the MFI of HLA-DR were smaller in the group of patients with schistosomiasis compared to the group of patients with leishmaniasis. On the other hand, the frequency of IL-10R on MoDCs was higher in patients with schistosomiasis than in patients with leishmaniasis. CD4+ and CD8+ T lymphocytes from patients with schistosomiasis presented a lower frequency of CD28 and a higher frequency of CTLA-4 compared to lymphocytes from patients with leishmaniasis. Levels of IL-10 were higher in the supernatants of co-cultures from individuals with schistosomiasis compared to those with leishmaniasis. However, levels of TNF, IL-12p40, and IFN-γ were lower in the group of individuals with schistosomiasis. Regarding the frequency of MoDCs infected by L. braziliensis after 72h in culture, it was observed that higher frequencies of cells from patients with schistosomiasis were infected compared to cells from patients with leishmaniasis. It was concluded that MoDCs from patients with schistosomiasis are more likely to be infected by L. braziliensis, possibly due to a lower degree of activation and a regulatory profile.engElsevierEsquistossomoseLeishmanioseCélulas dendríticasLeishmania braziliensisSchistosomiasisLeishmaniasisDendritic cellsLeishmania braziliensisSusceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27191/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLopes DM Susceptibility of dendritic cells from individuals ....pdfLopes DM Susceptibility of dendritic cells from individuals 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| dc.title.pt_BR.fl_str_mv |
Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis |
| title |
Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis |
| spellingShingle |
Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis Lopes, Diego Mota Esquistossomose Leishmaniose Células dendríticas Leishmania braziliensis Schistosomiasis Leishmaniasis Dendritic cells Leishmania braziliensis |
| title_short |
Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis |
| title_full |
Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis |
| title_fullStr |
Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis |
| title_full_unstemmed |
Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis |
| title_sort |
Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis |
| author |
Lopes, Diego Mota |
| author_facet |
Lopes, Diego Mota Almeida, Tarcísio Vila Verde Santana de Souza, Robson da Paixão de Ribeiro, Luís Eduardo Viana Page, Brady Fernandes, Jamille de Souza Carvalho Filho, Edgar Marcelino Cardoso, Luciana Santos |
| author_role |
author |
| author2 |
Almeida, Tarcísio Vila Verde Santana de Souza, Robson da Paixão de Ribeiro, Luís Eduardo Viana Page, Brady Fernandes, Jamille de Souza Carvalho Filho, Edgar Marcelino Cardoso, Luciana Santos |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lopes, Diego Mota Almeida, Tarcísio Vila Verde Santana de Souza, Robson da Paixão de Ribeiro, Luís Eduardo Viana Page, Brady Fernandes, Jamille de Souza Carvalho Filho, Edgar Marcelino Cardoso, Luciana Santos |
| dc.subject.other.pt_BR.fl_str_mv |
Esquistossomose Leishmaniose Células dendríticas Leishmania braziliensis |
| topic |
Esquistossomose Leishmaniose Células dendríticas Leishmania braziliensis Schistosomiasis Leishmaniasis Dendritic cells Leishmania braziliensis |
| dc.subject.en.pt_BR.fl_str_mv |
Schistosomiasis Leishmaniasis Dendritic cells Leishmania braziliensis |
| description |
CNPq/MST/INCT-DT, grant number 465229/2014-0 and National Institutes of Health, grant number: U01 AI136032. |
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2018 |
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2018-06-29T13:48:58Z |
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2018-06-29T13:48:58Z |
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2018 |
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LOPES, D. M. et al. Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis. Molecular Immunology, v. 93, p. 173–183, 2018. |
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https://www.arca.fiocruz.br/handle/icict/27191 |
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0161-5890 |
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10.1016/j.molimm.2017.11.018 |
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LOPES, D. M. et al. Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis. Molecular Immunology, v. 93, p. 173–183, 2018. 0161-5890 10.1016/j.molimm.2017.11.018 |
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