A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum

Detalhes bibliográficos
Autor(a) principal: Jesus, Joílson Ramos de
Data de Publicação: 2016
Outros Autores: Pontes-de-Carvalho, Lain Carlos, Melo, Stella Maria Barrouin, Neves, Neuza Maria Alcântara, Dutra, Rosa F
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15563
Resumo: National Council of Scientific and Technological Development (CNPq).
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spelling Jesus, Joílson Ramos dePontes-de-Carvalho, Lain CarlosMelo, Stella Maria BarrouinNeves, Neuza Maria AlcântaraDutra, Rosa F2016-08-31T17:16:02Z2016-08-31T17:16:02Z2016JESUS, J. R. et al. A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum. Biochemical Engineering Journal, v. 110, p. 43–50, 2016.1369-703Xhttps://www.arca.fiocruz.br/handle/icict/15563National Council of Scientific and Technological Development (CNPq).Federal University of Pernambuco. Biomedical Engineering Laboratory. Recife, PE, Brasil / Federal University of Piauí. UFPI. Biodiversity and Biotechnology Research Group. Parnaíba, PI, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University Bahia. Department of Veterinary. Salvador, BA, BrasilFederal University of Bahia. Department of Biointeraction. Salvador, BA, BrasilFederal University of Pernambuco. Biomedical Engineering Laboratory. Recife, PE, BrasilVisceral leishmaniasis (VL) is a severe systemic and infectious disease potentially fatal when undiagnosed or untreated. So far, accurate diagnosis is still a problem, especially in endemic areas and in the tracking and screening of asymptomatic dogs, which are good reservoirs and main host in urban areas. Recombinant antigens based on DNA technology have provided more reliable serological diagnostics since the specificity can be achieved easier than using whole extracts. This work reports a sensitive piezeolectric immunosensor for anti-Leishamnia antibodies based on rLci2B recombinant antigens immobilized on quartz crystal electrode. The electrode surface was assembled by a nafion film recovered by gold nanoparticles (AuNPs) to promote a greater amount of rLci2B due to the increase of surface area and stability on bonds. The immunosensor distinguished the positive VL canine serum showing a good linearity (r = 0.989; p << 0.01) and a low relative error (<5%) at 1:400 to 1:1600 serum dilutions. The system based on AuNP achieved better results regarding sensitivity and reproducibility than the cysteamine-based immunosensor without AuNP (r = 0.769; p = 0.147). The results obtained show as a promising tool for diagnosis of the VL.engElsevierA gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine seruminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleImmunosensorQuartz crystal microbalanceLeishmaniaLeishmania infantuminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15563/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJesus JR A gold nanoparticle....pdfJesus JR A gold nanoparticle....pdfapplication/pdf1667093https://www.arca.fiocruz.br/bitstream/icict/15563/2/Jesus%20JR%20A%20gold%20nanoparticle....pdf3c0bc04860a31b03dd5ac316907ed1dfMD52TEXTJesus JR A gold nanoparticle....pdf.txtJesus JR A gold nanoparticle....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum
title A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum
spellingShingle A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum
Jesus, Joílson Ramos de
Immunosensor
Quartz crystal microbalance
Leishmania
Leishmania infantum
title_short A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum
title_full A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum
title_fullStr A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum
title_full_unstemmed A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum
title_sort A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum
author Jesus, Joílson Ramos de
author_facet Jesus, Joílson Ramos de
Pontes-de-Carvalho, Lain Carlos
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa F
author_role author
author2 Pontes-de-Carvalho, Lain Carlos
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa F
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Jesus, Joílson Ramos de
Pontes-de-Carvalho, Lain Carlos
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa F
dc.subject.en.pt_BR.fl_str_mv Immunosensor
Quartz crystal microbalance
Leishmania
Leishmania infantum
topic Immunosensor
Quartz crystal microbalance
Leishmania
Leishmania infantum
description National Council of Scientific and Technological Development (CNPq).
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-08-31T17:16:02Z
dc.date.available.fl_str_mv 2016-08-31T17:16:02Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv JESUS, J. R. et al. A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum. Biochemical Engineering Journal, v. 110, p. 43–50, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15563
dc.identifier.issn.pt_BR.fl_str_mv 1369-703X
identifier_str_mv JESUS, J. R. et al. A gold nanoparticle piezoelectric immunosensor using a recombinant antigen for detecting Leishmania infantum antibodies in canine serum. Biochemical Engineering Journal, v. 110, p. 43–50, 2016.
1369-703X
url https://www.arca.fiocruz.br/handle/icict/15563
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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