A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.

Detalhes bibliográficos
Autor(a) principal: Jesus, Joílson Ramos de
Data de Publicação: 2011
Outros Autores: Carvalho, Kellyanne dos Anjos, Fonseca, Rosana Anita da Silva, Oliveira, Geraldo Gileno de Sá, Melo, Stella Maria Barrouin, Neves, Neuza Maria Alcântara, Dutra, Rosa Amalia Fireman
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8448
Resumo: Universidade Federal de Pernambuco. Laboratório de Engenharia Biomédica. Recife, PE, Brasil / Universidade Federal da Bahia. Departamento de Biointeração. Salvador, BA, Brasil
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spelling Jesus, Joílson Ramos deCarvalho, Kellyanne dos AnjosFonseca, Rosana Anita da SilvaOliveira, Geraldo Gileno de SáMelo, Stella Maria BarrouinNeves, Neuza Maria AlcântaraDutra, Rosa Amalia Fireman2014-09-25T17:28:33Z2014-09-25T17:28:33Z2011RAMOS-JESUS, J. et al. A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum. Analytical and Bioanalytical Chemistry, v. 401, n. 3, p. 917-925, 2011.1618-2650https://www.arca.fiocruz.br/handle/icict/844810.1007/s00216-011-5136-7engSpringerA piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Pernambuco. Laboratório de Engenharia Biomédica. Recife, PE, Brasil / Universidade Federal da Bahia. Departamento de Biointeração. Salvador, BA, BrasilUniversidade Federal da Bahia. Departamento de Biointeração. Salvador, BA, BrasilUniversidade Federal de Pernambuco. Laboratório de Engenharia Biomédica. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Departamento de Patologia e Clínicas. Salvador, BA, BrasilUniversidade Federal da Bahia. Departamento de Biointeração. Salvador, BA, BrasilUniversidade Federal de Pernambuco. Laboratório de Engenharia Biomédica. Recife, PE, BrasilThe American visceral leishmaniasis is an important cause of morbidity and mortality in Brazil for both humans and dogs. Attempts to make a diagnosis of this disease need to be improved, especially in endemic areas, and in the tracking and screening of asymptomatic dogs, which are their main host in urban areas. A quartz crystal microbalance immunosensor for the diagnosis of the canine visceral leishmaniasis using a recombinant antigen of Leishmania chagasi (rLci2B-NH6) was developed. The rLci2B-NH6 was tightly immobilized on a quartz crystal gold electrode by self-assembled monolayer based on short-chain length thiol. The strategy was the use of the antigen-histidine tail covalently linked to glutaraldehyde performing a Schift base which permits a major exposure of epitopes and a reduced steric hindrance. The immunosensor showed good results regarding sensitivity and reproducibility, being able to distinguish positive and negative canine serum for L. chagasi. Furthermore, the immunosensor can be reused through exposure to sodium dodecyl sulfate solution, which promotes the dissociation of antigen-antibody binding, restoring the sensor surface with immobilized biologically active antigens for further analysisLeishmania chagasiImmunosensorSelf-assembled monolayerPiezoelectricAnticorpos Antiprotozoários/sangueBioensaio/instrumentaçãoLeishmaniose Visceral/sangueAnimaisAntígenos/químicaCãesEletrodosLeishmania infantum/imunologiaLeishmaniose Visceral/imunologiaSistemas Micro-Eletromecânicos/métodosQuartzoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8448/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALRamos-Jesus, J A piezoelectric....pdfRamos-Jesus, J A piezoelectric....pdfapplication/pdf437754https://www.arca.fiocruz.br/bitstream/icict/8448/2/Ramos-Jesus%2c%20J%20A%20piezoelectric....pdf31bddac29e0486563f7c05bf3b84bd65MD52TEXTRamos-Jesus, J A piezoelectric....pdf.txtRamos-Jesus, J A piezoelectric....pdf.txtExtracted texttext/plain38308https://www.arca.fiocruz.br/bitstream/icict/8448/3/Ramos-Jesus%2c%20J%20A%20piezoelectric....pdf.txt40f20c4283ca155714afdeecdb49ce8bMD53icict/84482023-03-15 14:33:18.832oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.
title A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.
spellingShingle A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.
Jesus, Joílson Ramos de
Leishmania chagasi
Immunosensor
Self-assembled monolayer
Piezoelectric
Anticorpos Antiprotozoários/sangue
Bioensaio/instrumentação
Leishmaniose Visceral/sangue
Animais
Antígenos/química
Cães
Eletrodos
Leishmania infantum/imunologia
Leishmaniose Visceral/imunologia
Sistemas Micro-Eletromecânicos/métodos
Quartzo
title_short A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.
title_full A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.
title_fullStr A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.
title_full_unstemmed A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.
title_sort A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum.
author Jesus, Joílson Ramos de
author_facet Jesus, Joílson Ramos de
Carvalho, Kellyanne dos Anjos
Fonseca, Rosana Anita da Silva
Oliveira, Geraldo Gileno de Sá
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa Amalia Fireman
author_role author
author2 Carvalho, Kellyanne dos Anjos
Fonseca, Rosana Anita da Silva
Oliveira, Geraldo Gileno de Sá
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa Amalia Fireman
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jesus, Joílson Ramos de
Carvalho, Kellyanne dos Anjos
Fonseca, Rosana Anita da Silva
Oliveira, Geraldo Gileno de Sá
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa Amalia Fireman
dc.subject.en.pt_BR.fl_str_mv Leishmania chagasi
Immunosensor
Self-assembled monolayer
Piezoelectric
topic Leishmania chagasi
Immunosensor
Self-assembled monolayer
Piezoelectric
Anticorpos Antiprotozoários/sangue
Bioensaio/instrumentação
Leishmaniose Visceral/sangue
Animais
Antígenos/química
Cães
Eletrodos
Leishmania infantum/imunologia
Leishmaniose Visceral/imunologia
Sistemas Micro-Eletromecânicos/métodos
Quartzo
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Antiprotozoários/sangue
Bioensaio/instrumentação
Leishmaniose Visceral/sangue
Animais
Antígenos/química
Cães
Eletrodos
Leishmania infantum/imunologia
Leishmaniose Visceral/imunologia
Sistemas Micro-Eletromecânicos/métodos
Quartzo
description Universidade Federal de Pernambuco. Laboratório de Engenharia Biomédica. Recife, PE, Brasil / Universidade Federal da Bahia. Departamento de Biointeração. Salvador, BA, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-09-25T17:28:33Z
dc.date.available.fl_str_mv 2014-09-25T17:28:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAMOS-JESUS, J. et al. A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum. Analytical and Bioanalytical Chemistry, v. 401, n. 3, p. 917-925, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8448
dc.identifier.issn.none.fl_str_mv 1618-2650
dc.identifier.doi.none.fl_str_mv 10.1007/s00216-011-5136-7
identifier_str_mv RAMOS-JESUS, J. et al. A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum. Analytical and Bioanalytical Chemistry, v. 401, n. 3, p. 917-925, 2011.
1618-2650
10.1007/s00216-011-5136-7
url https://www.arca.fiocruz.br/handle/icict/8448
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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