Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50190 |
Resumo: | Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), 40296-710 Salvador, Bahia, Brazil or, e-mail: danielpbezerra@gmail.com; daniel.bezerra@fiocruz.br b Metabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA), 690065-130 Manaus, Amazonas, Brazil or, e-mail: hectorkoolen@gmail.com; hkoolen@uea.edu.br c School of Medicine and School of Dentistry, Federal University of Bahia, 40301-155 Salvador, Bahia, Brazil d Laboratory of Applied Chemistry and Technology, Amazonas State University (UEA), 69050-020 Manaus, Amazonas, Brazil e Faculty of Pharmacy, Federal University of Amazonas (UFAM), 69077-000 Manaus, Amazonas, Brazil f Laboratory of Plant Physiology and Biochemistry, National Institute for Amazonian Research (INPA), 69011-970 Manaus, Amazonas, Brazil g Department of Chemistry, Federal University of Amazonas (UFAM), 69080-900 Manaus, Amazonas, Brazil h Analytical Center, Multidisciplinary Support Center (CAM), Federal University of Amazonas (UFAM), 69080-900 Manaus, Amazonas, Brazil |
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Oliveira, Felipe P. deRodrigues, Ana Carolina B. da C.Lima, Emilly J. S. P. deSilva, Valdenizia R.Santos, Luciano de S.Anunciação, Talita A. daNogueira, Mateus L.Soares, Milena Botelho PereiraDias, Rosane B.Rocha, Clarissa Araujo GurgelDuvoisin Junior, SérgioAlbuquerque, Patrícia M.Lima, Emerson S.Gonçalves, José F. C.Bataglion, Giovana A.Costa, Emmanoel V.Silva, Felipe M. A. daKoolen, Hector H. F.Bezerra, Daniel Pereira2021-12-07T11:17:51Z2021-12-07T11:17:51Z2021OLIVEIRA, Felipe P. de et al. Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model. Chemistry and Biodiversity, 2021.1612-1872https://www.arca.fiocruz.br/handle/icict/5019010.1002/cbdv.202000938Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), 40296-710 Salvador, Bahia, Brazil or, e-mail: danielpbezerra@gmail.com; daniel.bezerra@fiocruz.br b Metabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA), 690065-130 Manaus, Amazonas, Brazil or, e-mail: hectorkoolen@gmail.com; hkoolen@uea.edu.br c School of Medicine and School of Dentistry, Federal University of Bahia, 40301-155 Salvador, Bahia, Brazil d Laboratory of Applied Chemistry and Technology, Amazonas State University (UEA), 69050-020 Manaus, Amazonas, Brazil e Faculty of Pharmacy, Federal University of Amazonas (UFAM), 69077-000 Manaus, Amazonas, Brazil f Laboratory of Plant Physiology and Biochemistry, National Institute for Amazonian Research (INPA), 69011-970 Manaus, Amazonas, Brazil g Department of Chemistry, Federal University of Amazonas (UFAM), 69080-900 Manaus, Amazonas, Brazil h Analytical Center, Multidisciplinary Support Center (CAM), Federal University of Amazonas (UFAM), 69080-900 Manaus, Amazonas, BrazilConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil, finance code 001); Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB, Brazil); and Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM, Brazil)."Múltipla ver em Notas"Aniba parviflora (MEISN.) MEZ (Lauraceae) is an aromatic plant of the Amazon rainforest, which has a tremendous commercial value in the perfumery industry; it is popularly used as flavoring sachets and aromatic baths. In Brazilian folk medicine, A. parviflora is used to treat victims of snakebites. Herein, we analyzed the chemical composition of A. parviflora bark essential oil (EO) and its effect on the growth of human hepatocellular carcinoma HepG2 cells in vitro and in vivo. EO was obtained by hydrodistillation and characterized by GC-MS and GC-FID. The main constituents of EO were linalool (16.3:3.15), α-humulene (14.5:2.41 %), δ-cadinene (10.2: 1.09 %), α-copaene (9.51:1.12%) and germacrene B (7.58:2.15 %). Initially, EO’s cytotoxic effect was evaluated against five cancer cell lines (HepG2, MCF-7, HCT116, HL-60 and B16-F10) and one non-cancerous one (MRC-5), using the Alamar blue method after 72 h of treatment. The calculated IC50 values were 9.05, 22.04, >50, 15.36, 17.57, and 30.46 μg/mL, respectively. The best selectivity was for HepG2 cells with a selective index of 3.4. DNA Fragmentation and cell cycle distribution were quantified in HepG2 cells by flow cytometry after a treatment period of 24 and 48 h. The effect of EO on tumor development in vivo was evaluated in a xenograft model using C.B-17 SCID mice engrafted with HepG2 cells. In vivo tumor growth inhibition of HepG2 xenograft at the doses of 40 and 80 mg/kg were 12.1 and 62.4 %, respectively.engWileyPlantasAgentes antitumoraisCitotoxicidadeCélulas Hep G2LauraceaeAniba parvifloraAntitumor agentsCytotoxicityHepG2LauraceaeEssential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/50190/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALOliveira, Felipe P. Essential....pdfOliveira, Felipe P. 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| dc.title.pt_BR.fl_str_mv |
Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model |
| title |
Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model |
| spellingShingle |
Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model Oliveira, Felipe P. de Plantas Agentes antitumorais Citotoxicidade Células Hep G2 Lauraceae Aniba parviflora Antitumor agents Cytotoxicity HepG2 Lauraceae |
| title_short |
Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model |
| title_full |
Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model |
| title_fullStr |
Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model |
| title_full_unstemmed |
Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model |
| title_sort |
Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model |
| author |
Oliveira, Felipe P. de |
| author_facet |
Oliveira, Felipe P. de Rodrigues, Ana Carolina B. da C. Lima, Emilly J. S. P. de Silva, Valdenizia R. Santos, Luciano de S. Anunciação, Talita A. da Nogueira, Mateus L. Soares, Milena Botelho Pereira Dias, Rosane B. Rocha, Clarissa Araujo Gurgel Duvoisin Junior, Sérgio Albuquerque, Patrícia M. Lima, Emerson S. Gonçalves, José F. C. Bataglion, Giovana A. Costa, Emmanoel V. Silva, Felipe M. A. da Koolen, Hector H. F. Bezerra, Daniel Pereira |
| author_role |
author |
| author2 |
Rodrigues, Ana Carolina B. da C. Lima, Emilly J. S. P. de Silva, Valdenizia R. Santos, Luciano de S. Anunciação, Talita A. da Nogueira, Mateus L. Soares, Milena Botelho Pereira Dias, Rosane B. Rocha, Clarissa Araujo Gurgel Duvoisin Junior, Sérgio Albuquerque, Patrícia M. Lima, Emerson S. Gonçalves, José F. C. Bataglion, Giovana A. Costa, Emmanoel V. Silva, Felipe M. A. da Koolen, Hector H. F. Bezerra, Daniel Pereira |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Felipe P. de Rodrigues, Ana Carolina B. da C. Lima, Emilly J. S. P. de Silva, Valdenizia R. Santos, Luciano de S. Anunciação, Talita A. da Nogueira, Mateus L. Soares, Milena Botelho Pereira Dias, Rosane B. Rocha, Clarissa Araujo Gurgel Duvoisin Junior, Sérgio Albuquerque, Patrícia M. Lima, Emerson S. Gonçalves, José F. C. Bataglion, Giovana A. Costa, Emmanoel V. Silva, Felipe M. A. da Koolen, Hector H. F. Bezerra, Daniel Pereira |
| dc.subject.other.pt_BR.fl_str_mv |
Plantas Agentes antitumorais Citotoxicidade Células Hep G2 Lauraceae |
| topic |
Plantas Agentes antitumorais Citotoxicidade Células Hep G2 Lauraceae Aniba parviflora Antitumor agents Cytotoxicity HepG2 Lauraceae |
| dc.subject.en.pt_BR.fl_str_mv |
Aniba parviflora Antitumor agents Cytotoxicity HepG2 Lauraceae |
| description |
Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), 40296-710 Salvador, Bahia, Brazil or, e-mail: danielpbezerra@gmail.com; daniel.bezerra@fiocruz.br b Metabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA), 690065-130 Manaus, Amazonas, Brazil or, e-mail: hectorkoolen@gmail.com; hkoolen@uea.edu.br c School of Medicine and School of Dentistry, Federal University of Bahia, 40301-155 Salvador, Bahia, Brazil d Laboratory of Applied Chemistry and Technology, Amazonas State University (UEA), 69050-020 Manaus, Amazonas, Brazil e Faculty of Pharmacy, Federal University of Amazonas (UFAM), 69077-000 Manaus, Amazonas, Brazil f Laboratory of Plant Physiology and Biochemistry, National Institute for Amazonian Research (INPA), 69011-970 Manaus, Amazonas, Brazil g Department of Chemistry, Federal University of Amazonas (UFAM), 69080-900 Manaus, Amazonas, Brazil h Analytical Center, Multidisciplinary Support Center (CAM), Federal University of Amazonas (UFAM), 69080-900 Manaus, Amazonas, Brazil |
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2021 |
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2021-12-07T11:17:51Z |
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2021-12-07T11:17:51Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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OLIVEIRA, Felipe P. de et al. Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model. Chemistry and Biodiversity, 2021. |
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https://www.arca.fiocruz.br/handle/icict/50190 |
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1612-1872 |
| dc.identifier.doi.none.fl_str_mv |
10.1002/cbdv.202000938 |
| identifier_str_mv |
OLIVEIRA, Felipe P. de et al. Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model. Chemistry and Biodiversity, 2021. 1612-1872 10.1002/cbdv.202000938 |
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eng |
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Wiley |
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Wiley |
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