Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model
| Autor(a) principal: | |
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| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42459 |
Resumo: | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM); and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). |
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Nogueira, Mateus LimaLima, Emilly J. S. P. deAdrião, Asenate A. X.Fontes, Sheila S.Silva, Valdenizia RodriguesSantos, Luciano de S.Soares, Milena Botelho PereiraDias, Rosane BorgesRocha, Clarissa Araújo GurgelCosta, Emmanoel VilaçaSilva, Felipe Moura Araujo daSantos, Marcos André VannierCardozo, Nállarett M. D.Koolen, Hector Henrique FerreiraBezerra, Daniel Pereira2020-07-30T13:37:48Z2020-07-30T13:37:48Z2020NOGUEIRA, Mateus Lima et al. Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model. Molecules, v. 25, 2020.1420-3049https://www.arca.fiocruz.br/handle/icict/4245910.3390/molecules25112687Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM); and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Amazonas, MA, Brazil.Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Amazonas, MA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Clinical Propaedeutics and Integrated Clinical. Bahia, Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Clinical Propaedeutics and Integrated Clinical. Bahia, Salvador, BA, Brazil.Federal University of Amazonas. Department of Chemistry. Amazonas, MA, Brazil.Federal University of Amazonas. Department of Chemistry. Amazonas, MA, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Amazonia Museum. Amazonas, MA, Brazil.Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Amazonas, MA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Cyperus articulatus L. (Cyperaceae), popularly known in Brazil as "priprioca" or "piriprioca", is a tropical and subtropical plant used in popular medical practices to treat many diseases, including cancer. In this study, C. articulatus rhizome essential oil (EO), collected from the Brazilian Amazon rainforest, was addressed in relation to its chemical composition, induction of cell death in vitro and inhibition of tumor development in vivo, using human hepatocellular carcinoma HepG2 cells as a cell model. EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized qualitatively and quantitatively by gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detection (GC-FID), respectively. The cytotoxic activity of EO was examined against five cancer cell lines (HepG2, HCT116, MCF-7, HL-60 and B16-F10) and one non-cancerous one (MRC-5) using the Alamar blue assay. Cell cycle distribution and cell death were investigated using flow cytometry in HepG2 cells treated with EO after 24, 48 and 72 h of incubation. The cells were also stained with May-Grunwald-Giemsa to analyze the morphological changes. The anti-liver-cancer activity of EO in vivo was evaluated in C.B-17 severe combined immunodeficient (SCID) mice with HepG2 cell xenografts. The main representative substances of this EO sample were muskatone (11.6%), cyclocolorenone (10.3%), α-pinene (8.26%), pogostol (6.36%), α-copaene (4.83%) and caryophyllene oxide (4.82%). EO showed IC50 values for cancer cell lines ranging from 28.5 µg/mL for HepG2 to >50 µg/mL for HCT116, and an IC50 value for non-cancerous of 46.0 µg/mL (MRC-5), showing selectivity indices below 2-fold for all cancer cells tested. HepG2 cells treated with EO showed cell cycle arrest at G2/M along with internucleosomal DNA fragmentation. The morphological alterations included cell shrinkage and chromatin condensation. Treatment with EO also increased the percentage of apoptotic-like cells. The in vivo tumor mass inhibition rates of EO were 46.5-50.0%. The results obtained indicate the anti-liver-cancer potential of C. articulatus rhizome EO.engMDPICyperus articulaMorte celularAntitumorCarcinoma hepatocelularCyperus articulatesCell deathG2/M arrestHepG2AntitumorCyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42459/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALNogueira Mateus L. Cyperus....pdfNogueira Mateus L. 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| dc.title.pt_BR.fl_str_mv |
Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model |
| title |
Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model |
| spellingShingle |
Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model Nogueira, Mateus Lima Cyperus articula Morte celular Antitumor Carcinoma hepatocelular Cyperus articulates Cell death G2/M arrest HepG2 Antitumor |
| title_short |
Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model |
| title_full |
Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model |
| title_fullStr |
Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model |
| title_full_unstemmed |
Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model |
| title_sort |
Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model |
| author |
Nogueira, Mateus Lima |
| author_facet |
Nogueira, Mateus Lima Lima, Emilly J. S. P. de Adrião, Asenate A. X. Fontes, Sheila S. Silva, Valdenizia Rodrigues Santos, Luciano de S. Soares, Milena Botelho Pereira Dias, Rosane Borges Rocha, Clarissa Araújo Gurgel Costa, Emmanoel Vilaça Silva, Felipe Moura Araujo da Santos, Marcos André Vannier Cardozo, Nállarett M. D. Koolen, Hector Henrique Ferreira Bezerra, Daniel Pereira |
| author_role |
author |
| author2 |
Lima, Emilly J. S. P. de Adrião, Asenate A. X. Fontes, Sheila S. Silva, Valdenizia Rodrigues Santos, Luciano de S. Soares, Milena Botelho Pereira Dias, Rosane Borges Rocha, Clarissa Araújo Gurgel Costa, Emmanoel Vilaça Silva, Felipe Moura Araujo da Santos, Marcos André Vannier Cardozo, Nállarett M. D. Koolen, Hector Henrique Ferreira Bezerra, Daniel Pereira |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nogueira, Mateus Lima Lima, Emilly J. S. P. de Adrião, Asenate A. X. Fontes, Sheila S. Silva, Valdenizia Rodrigues Santos, Luciano de S. Soares, Milena Botelho Pereira Dias, Rosane Borges Rocha, Clarissa Araújo Gurgel Costa, Emmanoel Vilaça Silva, Felipe Moura Araujo da Santos, Marcos André Vannier Cardozo, Nállarett M. D. Koolen, Hector Henrique Ferreira Bezerra, Daniel Pereira |
| dc.subject.other.pt_BR.fl_str_mv |
Cyperus articula Morte celular Antitumor Carcinoma hepatocelular |
| topic |
Cyperus articula Morte celular Antitumor Carcinoma hepatocelular Cyperus articulates Cell death G2/M arrest HepG2 Antitumor |
| dc.subject.en.pt_BR.fl_str_mv |
Cyperus articulates Cell death G2/M arrest HepG2 Antitumor |
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM); and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). |
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2020 |
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2020-07-30T13:37:48Z |
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2020-07-30T13:37:48Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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NOGUEIRA, Mateus Lima et al. Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model. Molecules, v. 25, 2020. |
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https://www.arca.fiocruz.br/handle/icict/42459 |
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1420-3049 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/molecules25112687 |
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NOGUEIRA, Mateus Lima et al. Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model. Molecules, v. 25, 2020. 1420-3049 10.3390/molecules25112687 |
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