L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood flow in experimental cerebral malaria

Detalhes bibliográficos
Autor(a) principal: Moreira, Aline S.
Data de Publicação: 2019
Outros Autores: Estato, Vanessa, Malvar, David C., Sanches, Guilherme S., Gomes, Fabiana, Tibiriçá, Eduardo, Daniel-Ribeiro, Cláudio Tadeu, Carvalho, Leonardo J. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/41074
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
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spelling Moreira, Aline S.Estato, VanessaMalvar, David C.Sanches, Guilherme S.Gomes, FabianaTibiriçá, EduardoDaniel-Ribeiro, Cláudio TadeuCarvalho, Leonardo J. M.2020-05-03T19:03:12Z2020-05-03T19:03:12Z2019MOREIRA, Aline S. et al. L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood fow in experimental cerebral malaria. Scientifc Reports, v. 9, p. 1-13, Sept. 2019.2045-2322https://www.arca.fiocruz.br/handle/icict/4107410.1038/s41598-019-49855-xengNature ResearchSuplementação de L-argininaTromboxano sintaseFluxo sanguíneo cerebralMalária cerebral experimentalInibiçãoL-arginine supplementationThromboxane synthaseInhibitionCerebral blood fowExperimental cerebral malariaL-arginine supplementation and thromboxane synthase inhibition increases cerebral blood flow in experimental cerebral malariainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Departamento de Ciências Fisiológicas. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Cardiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Cerebral malaria pathogenesis involves vascular dysfunction with low nitric oxide (NO) bioavailability, vasoconstriction and impaired vasodilation, leading to ischemia, tissue hypoxia and ultimately death. Cerebral blood flow (CBF) involves NO and other pathways, including arachidonic acid (AA)-derived metabolites. Here we show that mice with experimental cerebral malaria (ECM) by P. berghei ANKA showed marked decreases in CBF (as assessed by laser speckle contrast imaging - LSCI) and that administration of L-arginine supplementation (50 mg/kg) and/or of the thromboxane synthase inhibitor Ozagrel (100 mg/kg) induced immediate increases in CBF. L-arginine in combination with artesunate (32 mg/kg) induced immediate reversal of brain ischemia in the short-term (1 hour), but the effect subsided after 3 and 6 hours. Neither L-arginine nor Ozagrel reversed blood brain barrier breakdown. Mice with ECM showed brain levels of selected AA-derived metabolites with a vasoconstrictor profile, with increased levels of 8-isoprostanes, 20-HETE and 14,15-DHET, whereas mice infected with a non-ECM-inducing strain of P. berghei (NK65) showed a vasodilator profile, with normal levels of 20-HETE and 14,15-DHET and increased levels of PGE2. L-arginine is capable of partially reversing cerebral ischemia and AA metabolites may play a role in the cerebrovascular dysfunction in ECM.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41074/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALClaudioTDRibeiro_GuilhermeSanchez_etal_IOC_2019.pdfClaudioTDRibeiro_GuilhermeSanchez_etal_IOC_2019.pdfapplication/pdf1595533https://www.arca.fiocruz.br/bitstream/icict/41074/2/ClaudioTDRibeiro_GuilhermeSanchez_etal_IOC_2019.pdfe484cdcf8c8c4d8a876e29ae75aad3fdMD52TEXTClaudioTDRibeiro_GuilhermeSanchez_etal_IOC_2019.pdf.txtClaudioTDRibeiro_GuilhermeSanchez_etal_IOC_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood flow in experimental cerebral malaria
title L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood flow in experimental cerebral malaria
spellingShingle L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood flow in experimental cerebral malaria
Moreira, Aline S.
Suplementação de L-arginina
Tromboxano sintase
Fluxo sanguíneo cerebral
Malária cerebral experimental
Inibição
L-arginine supplementation
Thromboxane synthase
Inhibition
Cerebral blood fow
Experimental cerebral malaria
title_short L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood flow in experimental cerebral malaria
title_full L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood flow in experimental cerebral malaria
title_fullStr L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood flow in experimental cerebral malaria
title_full_unstemmed L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood flow in experimental cerebral malaria
title_sort L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood flow in experimental cerebral malaria
author Moreira, Aline S.
author_facet Moreira, Aline S.
Estato, Vanessa
Malvar, David C.
Sanches, Guilherme S.
Gomes, Fabiana
Tibiriçá, Eduardo
Daniel-Ribeiro, Cláudio Tadeu
Carvalho, Leonardo J. M.
author_role author
author2 Estato, Vanessa
Malvar, David C.
Sanches, Guilherme S.
Gomes, Fabiana
Tibiriçá, Eduardo
Daniel-Ribeiro, Cláudio Tadeu
Carvalho, Leonardo J. M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moreira, Aline S.
Estato, Vanessa
Malvar, David C.
Sanches, Guilherme S.
Gomes, Fabiana
Tibiriçá, Eduardo
Daniel-Ribeiro, Cláudio Tadeu
Carvalho, Leonardo J. M.
dc.subject.other.pt_BR.fl_str_mv Suplementação de L-arginina
Tromboxano sintase
Fluxo sanguíneo cerebral
Malária cerebral experimental
Inibição
topic Suplementação de L-arginina
Tromboxano sintase
Fluxo sanguíneo cerebral
Malária cerebral experimental
Inibição
L-arginine supplementation
Thromboxane synthase
Inhibition
Cerebral blood fow
Experimental cerebral malaria
dc.subject.en.pt_BR.fl_str_mv L-arginine supplementation
Thromboxane synthase
Inhibition
Cerebral blood fow
Experimental cerebral malaria
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-05-03T19:03:12Z
dc.date.available.fl_str_mv 2020-05-03T19:03:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOREIRA, Aline S. et al. L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood fow in experimental cerebral malaria. Scientifc Reports, v. 9, p. 1-13, Sept. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/41074
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-019-49855-x
identifier_str_mv MOREIRA, Aline S. et al. L-arginine supplementation and thromboxane synthase inhibition increases cerebral blood fow in experimental cerebral malaria. Scientifc Reports, v. 9, p. 1-13, Sept. 2019.
2045-2322
10.1038/s41598-019-49855-x
url https://www.arca.fiocruz.br/handle/icict/41074
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/41074/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/41074/2/ClaudioTDRibeiro_GuilhermeSanchez_etal_IOC_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/41074/3/ClaudioTDRibeiro_GuilhermeSanchez_etal_IOC_2019.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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