Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28743 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil. |
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Lovo-Martins, Maria IsabelMalvezi, Aparecida DonizetteZanluqui, Nágela GhabdanLucchetti, Bruno Fernando CruzTatakihara, Vera Lúcia HidekoMorking, Patricia AlvesOliveira, Admilton Gonçalves deGoldenberg, SamuelWowk, Pryscilla FaniniPinge-Filho, Phileno2018-09-13T15:13:39Z2018-09-13T15:13:39Z2018LOVO-MARTINS, Maria Isabel et al. Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages. Frontiers in Immunology, v. 9, p. 1-16, 2018.1664-3224https://www.arca.fiocruz.br/handle/icict/2874310.3389/fimmu.2018.008961664-3224porFrontiers MediaVesículas ExtracelularesExtracellular VesiclesLipid DropletsMacrophagesProstaglandin E2Antigenic ModulationGotas LipídicasModulación AntigénicaGotículas LipídicasMacrófagosDinoprostonaModulação AntigênicaExtracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophagesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Central de Laboratórios de Pesquisa Multiusuários. Laboratório de Microscopia Eletrônica e Microanálises. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil.During the onset of Trypanosoma cruzi infection, an effective immune response is necessary to control parasite replication and ensure host survival. Macrophages have a central role in innate immunity, acting as an important trypanocidal cell and triggering the adaptive immune response through antigen presentation and cytokine production. However, T. cruzi displays immune evasion mechanisms that allow infection and replication in macrophages, favoring its chronic persistence. One potential mechanism is the release of T. cruzi strain Y extracellular vesicle (EV Y), which participate in intracellular communication by carrying functional molecules that signal host cells and can modulate the immune response. The present work aimed to evaluate immune modulation by EV Y in C57BL/6 mice, a prototype resistant to infection by T. cruzi strain Y, and the effects of direct EV Y stimulation of macrophages in vitro. EV Y inoculation in mice prior to T. cruzi infection resulted in increased parasitemia, elevated cardiac parasitism, decreased plasma nitric oxide (NO), reduced NO production by spleen cells, and modulation of cytokine production, with a reduction in TNF-α in plasma and decreased production of TNF-α and IL-6 by spleen cells from infected animals. In vitro assays using bone marrow-derived macrophages showed that stimulation with EV Y prior to infection by T. cruzi increased the parasite internalization rate and release of infective trypomastigotes by these cells. In this same scenario, EV Y induced lipid body formation and prostaglandin E2 (PGE2) production by macrophages even in the absence of T. cruzi. In infected macrophages, EV Y decreased production of PGE2 and cytokines TNF-α and IL-6 24 h after infection. These results suggest that EV Y modulates the host response in favor of the parasite and indicates a role for lipid bodies and PGE2 in immune modulation exerted by EVs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/28743/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALFrontiersImmunol-09-896.pdfFrontiersImmunol-09-896.pdfapplication/pdf4388180https://www.arca.fiocruz.br/bitstream/icict/28743/2/FrontiersImmunol-09-896.pdf78f9ff8768a5eb707450f4233383949dMD52TEXTFrontiersImmunol-09-896.pdf.txtFrontiersImmunol-09-896.pdf.txtExtracted texttext/plain86458https://www.arca.fiocruz.br/bitstream/icict/28743/3/FrontiersImmunol-09-896.pdf.txtc32ea3030dcd1cdafc4b09edd5d06b8cMD53icict/287432019-11-23 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| dc.title.pt_BR.fl_str_mv |
Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages |
| title |
Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages |
| spellingShingle |
Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages Lovo-Martins, Maria Isabel Vesículas Extracelulares Extracellular Vesicles Lipid Droplets Macrophages Prostaglandin E2 Antigenic Modulation Gotas Lipídicas Modulación Antigénica Gotículas Lipídicas Macrófagos Dinoprostona Modulação Antigênica |
| title_short |
Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages |
| title_full |
Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages |
| title_fullStr |
Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages |
| title_full_unstemmed |
Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages |
| title_sort |
Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages |
| author |
Lovo-Martins, Maria Isabel |
| author_facet |
Lovo-Martins, Maria Isabel Malvezi, Aparecida Donizette Zanluqui, Nágela Ghabdan Lucchetti, Bruno Fernando Cruz Tatakihara, Vera Lúcia Hideko Morking, Patricia Alves Oliveira, Admilton Gonçalves de Goldenberg, Samuel Wowk, Pryscilla Fanini Pinge-Filho, Phileno |
| author_role |
author |
| author2 |
Malvezi, Aparecida Donizette Zanluqui, Nágela Ghabdan Lucchetti, Bruno Fernando Cruz Tatakihara, Vera Lúcia Hideko Morking, Patricia Alves Oliveira, Admilton Gonçalves de Goldenberg, Samuel Wowk, Pryscilla Fanini Pinge-Filho, Phileno |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lovo-Martins, Maria Isabel Malvezi, Aparecida Donizette Zanluqui, Nágela Ghabdan Lucchetti, Bruno Fernando Cruz Tatakihara, Vera Lúcia Hideko Morking, Patricia Alves Oliveira, Admilton Gonçalves de Goldenberg, Samuel Wowk, Pryscilla Fanini Pinge-Filho, Phileno |
| dc.subject.other.pt_BR.fl_str_mv |
Vesículas Extracelulares |
| topic |
Vesículas Extracelulares Extracellular Vesicles Lipid Droplets Macrophages Prostaglandin E2 Antigenic Modulation Gotas Lipídicas Modulación Antigénica Gotículas Lipídicas Macrófagos Dinoprostona Modulação Antigênica |
| dc.subject.en.pt_BR.fl_str_mv |
Extracellular Vesicles Lipid Droplets Macrophages Prostaglandin E2 Antigenic Modulation |
| dc.subject.es.pt_BR.fl_str_mv |
Gotas Lipídicas Modulación Antigénica |
| dc.subject.decs.pt_BR.fl_str_mv |
Gotículas Lipídicas Macrófagos Dinoprostona Modulação Antigênica |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunopatologia Experimental. Londrina, PR, Brasil. |
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2018 |
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2018-09-13T15:13:39Z |
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2018-09-13T15:13:39Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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LOVO-MARTINS, Maria Isabel et al. Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages. Frontiers in Immunology, v. 9, p. 1-16, 2018. |
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https://www.arca.fiocruz.br/handle/icict/28743 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2018.00896 |
| dc.identifier.eissn.none.fl_str_mv |
1664-3224 |
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LOVO-MARTINS, Maria Isabel et al. Extracellular vesicles shed by Trypanosoma cruzi potentiate infection and elicit lipid body formation and PGE2 production in Murine Macrophages. Frontiers in Immunology, v. 9, p. 1-16, 2018. 1664-3224 10.3389/fimmu.2018.00896 |
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Frontiers Media |
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Frontiers Media |
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