Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1165/rcmb.2005-0145OC |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/41075 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
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Abreu, Adriana Vieira deAssis, Edson F.Gomes, Gleice S.Faria Neto, Hugo Caire C.Weller, Peter F.Melo, Christianne BandeiraBozza, Patrícia T.2020-05-03T19:27:09Z2020-05-03T19:27:09Z2005ABREU, Adriana Vieira de et al. Allergic Challenge–Elicited Lipid Bodies Compartmentalize In Vivo Leukotriene C4 Synthesis within Eosinophils. American Journal of Respiratory Cell and Molecular Biology, v. 33, p. 254-261, June 2005.1044-1549https://www.arca.fiocruz.br/handle/icict/4107510.1165/rcmb.2005-0145OC1535-4989engAmerican Thoracic SocietyAlergiaEosinófilosCorpos lipídicosLeukotriene C4AllergyCCR3EosinophilsLipid bodiesLTC4Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophilsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Harvard Medical School. Department of Medicine. Boston, MA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Eosinophils are an important source of leukotriene (LT)C(4), which can be synthesized within lipid bodies-cytoplasmic organelles where eicosanoid formation may take place. Allergy-driven lipid body formation and function have never been investigated. Here, we studied the in vivo induction and role of lipid bodies within eosinophils recruited to sites of allergic inflammation. Using two murine models of allergic inflammation (asthma and pleurisy), we verified that parallel to the eosinophil influx, allergic challenge also induced lipid body formation within recruited eosinophils. Neutralizing antibodies to eotaxin/CCL11, RANTES/CCL5, or CCR3 partially inhibited lipid body formation within recruited eosinophils in the allergic pleurisy model. Likewise, intrapleural administration of RANTES or eotaxin also induced significant influx of eosinophils loaded with lipid bodies. By immunolabeling, we detected the presence of a key enzyme involved in the leukotriene metabolism-5-lipoxygenase-within eosinophil lipid bodies formed in vivo after allergen challenge. Furthermore, specific immunolocalization of newly formed LTC(4) demonstrated that lipid bodies were the sites of formation of this eicosanoid within infiltrating eosinophils. Therefore, allergic inflammation triggers in vivo formation of new lipid bodies within infiltrating eosinophils, a phenomenon largely mediated by eotaxin/RANTES acting via CCR3 receptors. Such in vivo allergen-driven lipid bodies function as intracellular compartments of LTC(4) synthesis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41075/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGleiceGomes_AdrianaAbreu_etal_IOC_2005.pdfGleiceGomes_AdrianaAbreu_etal_IOC_2005.pdfapplication/pdf531629https://www.arca.fiocruz.br/bitstream/icict/41075/2/GleiceGomes_AdrianaAbreu_etal_IOC_2005.pdf4b2a403aeaabdd2228f91a0e019bd4cfMD52TEXTGleiceGomes_AdrianaAbreu_etal_IOC_2005.pdf.txtGleiceGomes_AdrianaAbreu_etal_IOC_2005.pdf.txtExtracted texttext/plain50447https://www.arca.fiocruz.br/bitstream/icict/41075/3/GleiceGomes_AdrianaAbreu_etal_IOC_2005.pdf.txt5a65afc2a84787d33e8942833f130a61MD53icict/410752022-06-24 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| dc.title.pt_BR.fl_str_mv |
Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils |
| title |
Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils |
| spellingShingle |
Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils Abreu, Adriana Vieira de Alergia Eosinófilos Corpos lipídicos Leukotriene C4 Allergy CCR3 Eosinophils Lipid bodies LTC4 |
| title_short |
Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils |
| title_full |
Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils |
| title_fullStr |
Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils |
| title_full_unstemmed |
Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils |
| title_sort |
Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils |
| author |
Abreu, Adriana Vieira de |
| author_facet |
Abreu, Adriana Vieira de Assis, Edson F. Gomes, Gleice S. Faria Neto, Hugo Caire C. Weller, Peter F. Melo, Christianne Bandeira Bozza, Patrícia T. |
| author_role |
author |
| author2 |
Assis, Edson F. Gomes, Gleice S. Faria Neto, Hugo Caire C. Weller, Peter F. Melo, Christianne Bandeira Bozza, Patrícia T. |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Abreu, Adriana Vieira de Assis, Edson F. Gomes, Gleice S. Faria Neto, Hugo Caire C. Weller, Peter F. Melo, Christianne Bandeira Bozza, Patrícia T. |
| dc.subject.other.pt_BR.fl_str_mv |
Alergia Eosinófilos Corpos lipídicos Leukotriene C4 |
| topic |
Alergia Eosinófilos Corpos lipídicos Leukotriene C4 Allergy CCR3 Eosinophils Lipid bodies LTC4 |
| dc.subject.en.pt_BR.fl_str_mv |
Allergy CCR3 Eosinophils Lipid bodies LTC4 |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
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2005 |
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2005 |
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2020-05-03T19:27:09Z |
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2020-05-03T19:27:09Z |
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ABREU, Adriana Vieira de et al. Allergic Challenge–Elicited Lipid Bodies Compartmentalize In Vivo Leukotriene C4 Synthesis within Eosinophils. American Journal of Respiratory Cell and Molecular Biology, v. 33, p. 254-261, June 2005. |
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https://www.arca.fiocruz.br/handle/icict/41075 |
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1044-1549 |
| dc.identifier.doi.none.fl_str_mv |
10.1165/rcmb.2005-0145OC |
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1535-4989 |
| identifier_str_mv |
ABREU, Adriana Vieira de et al. Allergic Challenge–Elicited Lipid Bodies Compartmentalize In Vivo Leukotriene C4 Synthesis within Eosinophils. American Journal of Respiratory Cell and Molecular Biology, v. 33, p. 254-261, June 2005. 1044-1549 10.1165/rcmb.2005-0145OC 1535-4989 |
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American Thoracic Society |
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