Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area

Detalhes bibliográficos
Autor(a) principal: Enk, Martin Johannes
Data de Publicação: 2012
Outros Autores: Silva, Guilherme Oliveira e, Rodrigues, Nilton Barnabé
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7431
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil/ Universidade Vale do Rio Doce. Laboratório de Parasitologia. Governador Valadares, MG, Brazil
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spelling Enk, Martin JohannesSilva, Guilherme Oliveira eRodrigues, Nilton Barnabé2014-03-21T12:28:54Z2014-03-21T12:28:54Z2012ENK, Johannes Enk ; OLIVEIRA SILVA, Guilherme; RODRIGUES, Nilton Barnabé. Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area. Plos One. 2012, vol.7, n.6, pp. e38947https://www.arca.fiocruz.br/handle/icict/743110.1371/journal.pone.0038947engPublic Library Of ScienceDiagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic areainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil/ Universidade Vale do Rio Doce. Laboratório de Parasitologia. Governador Valadares, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilUniversidade Vale do Rio Doce. Laboratório de Parasitologia. Governador Valadares, MG, Brazil/ Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilSchistosomiasis caused by Schistosoma mansoni, one of the most neglected human parasitoses in Latin America and Africa, is routinely confirmed by microscopic visualization of eggs in stool. The main limitation of this diagnostic approach is its lack of sensitivity in detecting individual low worm burdens and consequently data on infection rates in low transmission settings are little reliable. According to the scientific literature, PCR assays are characterized by high sensitivity and specificity in detecting parasite DNA in biological samples. A simple and cost effective extraction method for DNA of Schistosoma mansoni from urine samples in combination with a conventional PCR assay was developed and applied in an endemic area. This urine based PCR system was tested for diagnostic accuracy among a population of a small village in an endemic area, comparing it to a reference test composed of three different parasitological techniques. The diagnostic parameters revealed a sensitivity of 100%, a specificity of 91.20%, positive and negative predictive values of 86.25% and 100%, respectively, and a test accuracy of 94.33%. Further statistical analysis showed a k index of 0.8806, indicating an excellent agreement between the reference test and the PCR system. Data obtained from the mouse model indicate the infection can be detected one week after cercariae penetration, opening a new perspective for early detection and patient management during this stage of the disease. The data indicate that this innovative PCR system provides a simple to handle and robust diagnostic tool for the detection of S. mansoni DNA from urine samples and a promising approach to overcome the diagnostic obstacles in low transmission settings. Furthermore the principals of this molecular technique, based on the examination of human urine samples may be useful for the diagnosis of other neglected tropical diseases that can be detected by trans-renal DNA.Diagnostic medicineInfectious disease controlParasitic diseasesPolymerase chain reactionPolymerase chain reaction amplificationSchistosoma mansoniSchistosomiasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALDiagnostic Accuracy and Applicability of a PCR System for the Detection of Schistosoma mansoni DNA in Human Urine Samples from an Endemic Area.pdfDiagnostic Accuracy and Applicability of a PCR System for the Detection of Schistosoma mansoni DNA in Human Urine Samples from an Endemic Area.pdfapplication/pdf1605258https://www.arca.fiocruz.br/bitstream/icict/7431/1/Diagnostic%20Accuracy%20and%20Applicability%20of%20a%20PCR%20System%20for%20the%20Detection%20of%20Schistosoma%20mansoni%20DNA%20in%20Human%20Urine%20Samples%20from%20an%20Endemic%20Area.pdf7089316fd0c311394d034c28aafead58MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area
title Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area
spellingShingle Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area
Enk, Martin Johannes
Diagnostic medicine
Infectious disease control
Parasitic diseases
Polymerase chain reaction
Polymerase chain reaction amplification
Schistosoma mansoni
Schistosomiasis
title_short Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area
title_full Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area
title_fullStr Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area
title_full_unstemmed Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area
title_sort Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area
author Enk, Martin Johannes
author_facet Enk, Martin Johannes
Silva, Guilherme Oliveira e
Rodrigues, Nilton Barnabé
author_role author
author2 Silva, Guilherme Oliveira e
Rodrigues, Nilton Barnabé
author2_role author
author
dc.contributor.author.fl_str_mv Enk, Martin Johannes
Silva, Guilherme Oliveira e
Rodrigues, Nilton Barnabé
dc.subject.en.pt_BR.fl_str_mv Diagnostic medicine
Infectious disease control
Parasitic diseases
Polymerase chain reaction
Polymerase chain reaction amplification
Schistosoma mansoni
Schistosomiasis
topic Diagnostic medicine
Infectious disease control
Parasitic diseases
Polymerase chain reaction
Polymerase chain reaction amplification
Schistosoma mansoni
Schistosomiasis
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil/ Universidade Vale do Rio Doce. Laboratório de Parasitologia. Governador Valadares, MG, Brazil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-03-21T12:28:54Z
dc.date.available.fl_str_mv 2014-03-21T12:28:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ENK, Johannes Enk ; OLIVEIRA SILVA, Guilherme; RODRIGUES, Nilton Barnabé. Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area. Plos One. 2012, vol.7, n.6, pp. e38947
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7431
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0038947
identifier_str_mv ENK, Johannes Enk ; OLIVEIRA SILVA, Guilherme; RODRIGUES, Nilton Barnabé. Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area. Plos One. 2012, vol.7, n.6, pp. e38947
10.1371/journal.pone.0038947
url https://www.arca.fiocruz.br/handle/icict/7431
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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