Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7431 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil/ Universidade Vale do Rio Doce. Laboratório de Parasitologia. Governador Valadares, MG, Brazil |
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Enk, Martin JohannesSilva, Guilherme Oliveira eRodrigues, Nilton Barnabé2014-03-21T12:28:54Z2014-03-21T12:28:54Z2012ENK, Johannes Enk ; OLIVEIRA SILVA, Guilherme; RODRIGUES, Nilton Barnabé. Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area. Plos One. 2012, vol.7, n.6, pp. e38947https://www.arca.fiocruz.br/handle/icict/743110.1371/journal.pone.0038947engPublic Library Of ScienceDiagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic areainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil/ Universidade Vale do Rio Doce. Laboratório de Parasitologia. Governador Valadares, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilUniversidade Vale do Rio Doce. Laboratório de Parasitologia. Governador Valadares, MG, Brazil/ Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrazilSchistosomiasis caused by Schistosoma mansoni, one of the most neglected human parasitoses in Latin America and Africa, is routinely confirmed by microscopic visualization of eggs in stool. The main limitation of this diagnostic approach is its lack of sensitivity in detecting individual low worm burdens and consequently data on infection rates in low transmission settings are little reliable. According to the scientific literature, PCR assays are characterized by high sensitivity and specificity in detecting parasite DNA in biological samples. A simple and cost effective extraction method for DNA of Schistosoma mansoni from urine samples in combination with a conventional PCR assay was developed and applied in an endemic area. This urine based PCR system was tested for diagnostic accuracy among a population of a small village in an endemic area, comparing it to a reference test composed of three different parasitological techniques. The diagnostic parameters revealed a sensitivity of 100%, a specificity of 91.20%, positive and negative predictive values of 86.25% and 100%, respectively, and a test accuracy of 94.33%. Further statistical analysis showed a k index of 0.8806, indicating an excellent agreement between the reference test and the PCR system. Data obtained from the mouse model indicate the infection can be detected one week after cercariae penetration, opening a new perspective for early detection and patient management during this stage of the disease. The data indicate that this innovative PCR system provides a simple to handle and robust diagnostic tool for the detection of S. mansoni DNA from urine samples and a promising approach to overcome the diagnostic obstacles in low transmission settings. Furthermore the principals of this molecular technique, based on the examination of human urine samples may be useful for the diagnosis of other neglected tropical diseases that can be detected by trans-renal DNA.Diagnostic medicineInfectious disease controlParasitic diseasesPolymerase chain reactionPolymerase chain reaction amplificationSchistosoma mansoniSchistosomiasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALDiagnostic Accuracy and Applicability of a PCR System for the Detection of Schistosoma mansoni DNA in Human Urine Samples from an Endemic Area.pdfDiagnostic Accuracy and Applicability of a PCR System for the Detection of Schistosoma mansoni DNA in Human Urine Samples from an Endemic Area.pdfapplication/pdf1605258https://www.arca.fiocruz.br/bitstream/icict/7431/1/Diagnostic%20Accuracy%20and%20Applicability%20of%20a%20PCR%20System%20for%20the%20Detection%20of%20Schistosoma%20mansoni%20DNA%20in%20Human%20Urine%20Samples%20from%20an%20Endemic%20Area.pdf7089316fd0c311394d034c28aafead58MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area |
title |
Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area |
spellingShingle |
Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area Enk, Martin Johannes Diagnostic medicine Infectious disease control Parasitic diseases Polymerase chain reaction Polymerase chain reaction amplification Schistosoma mansoni Schistosomiasis |
title_short |
Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area |
title_full |
Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area |
title_fullStr |
Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area |
title_full_unstemmed |
Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area |
title_sort |
Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area |
author |
Enk, Martin Johannes |
author_facet |
Enk, Martin Johannes Silva, Guilherme Oliveira e Rodrigues, Nilton Barnabé |
author_role |
author |
author2 |
Silva, Guilherme Oliveira e Rodrigues, Nilton Barnabé |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Enk, Martin Johannes Silva, Guilherme Oliveira e Rodrigues, Nilton Barnabé |
dc.subject.en.pt_BR.fl_str_mv |
Diagnostic medicine Infectious disease control Parasitic diseases Polymerase chain reaction Polymerase chain reaction amplification Schistosoma mansoni Schistosomiasis |
topic |
Diagnostic medicine Infectious disease control Parasitic diseases Polymerase chain reaction Polymerase chain reaction amplification Schistosoma mansoni Schistosomiasis |
description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brazil/ Universidade Vale do Rio Doce. Laboratório de Parasitologia. Governador Valadares, MG, Brazil |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2014-03-21T12:28:54Z |
dc.date.available.fl_str_mv |
2014-03-21T12:28:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ENK, Johannes Enk ; OLIVEIRA SILVA, Guilherme; RODRIGUES, Nilton Barnabé. Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area. Plos One. 2012, vol.7, n.6, pp. e38947 |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/7431 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0038947 |
identifier_str_mv |
ENK, Johannes Enk ; OLIVEIRA SILVA, Guilherme; RODRIGUES, Nilton Barnabé. Diagnostic accuracy and applicability of a PCR system for the detection of Schistosoma mansoni DNA in human urine samples from an endemic area. Plos One. 2012, vol.7, n.6, pp. e38947 10.1371/journal.pone.0038947 |
url |
https://www.arca.fiocruz.br/handle/icict/7431 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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Public Library Of Science |
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Public Library Of Science |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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