Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats
Autor(a) principal: | |
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Data de Publicação: | 1988 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/4070 http://dx.doi.org/10.1590/S0036-46651988000500003 |
Resumo: | Female albino rats were used for the sequential histopathological study of experimental paracoccidioidomycosis. The animals were inoculated intraperitoneally with a strain of Paracoccidioides brasiliensis in the yeast-like phase, and sacrificed at given intervals from 1 to 168 days after inoculation; each animal received an inoculum of 4 x 10^ cells in 0.8 ml of saline. The control group received saline containing scrapings of the culture medium. Tissue from the inoculation site was examined. The cellular population, the extracellular matrix, and the presence and characteristics of fungi were analysed in the inflammatory granulomatous process by light microscopy. The results allowed to separate the kinetic of the inflammatory response into three stages: 1) neutrophilic or macrophagic-neutrophilic; 2) pre-granulomatous; 3) granulomatous. Synthesis of the extracellular matrix began with the depositing of fibrin-like material, and increased gradually with deposits of collagen, proteoglycans, and glycoproteins. Parasites were present in all of the examined periods. Recurrences of the disease were clearly shown through the concurrence of recently-formed granulomas with older granulomas, implying that this type of granulomatous process does not eliminate the disease, nor is it able to limit fungal dissemination over a prolonged period of time. |
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Kerr, I. B.Araripe, J. R.Oliveira, P. C.Lenzi, Henrique L,2012-05-15T15:17:48Z2012-05-15T15:17:48Z1988KERR, I. B. et al. Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats. Revista do Instituto de Medicina Tropical, v.30, n.5, p.336-350, Sept./Oct.1988.0036-4665https://www.arca.fiocruz.br/handle/icict/4070http://dx.doi.org/10.1590/S0036-46651988000500003Female albino rats were used for the sequential histopathological study of experimental paracoccidioidomycosis. The animals were inoculated intraperitoneally with a strain of Paracoccidioides brasiliensis in the yeast-like phase, and sacrificed at given intervals from 1 to 168 days after inoculation; each animal received an inoculum of 4 x 10^ cells in 0.8 ml of saline. The control group received saline containing scrapings of the culture medium. Tissue from the inoculation site was examined. The cellular population, the extracellular matrix, and the presence and characteristics of fungi were analysed in the inflammatory granulomatous process by light microscopy. The results allowed to separate the kinetic of the inflammatory response into three stages: 1) neutrophilic or macrophagic-neutrophilic; 2) pre-granulomatous; 3) granulomatous. Synthesis of the extracellular matrix began with the depositing of fibrin-like material, and increased gradually with deposits of collagen, proteoglycans, and glycoproteins. Parasites were present in all of the examined periods. Recurrences of the disease were clearly shown through the concurrence of recently-formed granulomas with older granulomas, implying that this type of granulomatous process does not eliminate the disease, nor is it able to limit fungal dissemination over a prolonged period of time.Female albino rats were used for the sequential histopathological study of experimental paracoccidioidomycosis. The animals were inoculated intraperitoneally with a strain of Paracoccidioides brasiliensis in the yeast-like phase, and sacrificed at given intervals from 1 to 168 days after inoculation; each animal received an inoculum of 4 x 106 cells in 0.8 ml of saline. The control group received saline containing scrapings of the culture medium. Tissue from the inoculation site was examined. The cellular population, the extracellular matrix, and the presence and characteristics of fungi were analysed in the inflammatory granulomatous process by light microscopy. The results allowed to separate the kinetic of the inflammatory response into three stages: 1) neutrophilic or macrophagic-neutrophilic; 2) pre-granulomatous; 3) granulomatous. Synthesis of the extracellular matrix began with the depositing of fibrin-like material, and increased gradually with deposits of collagen, proteoglycans, and glycoproteins. Parasites were present in all of the examined periods. Recurrences of the disease were clearly shown through the concurrence of recently-formed granulomas with older granulomas, implying that this type of granulomatous process does not eliminate the disease, nor is it able to limit fungal dissemination over a prolonged period of time.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.engInstituto de Medicina Tropical de São PauloParacoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected ratsParacoccidioidomicose: estudo histopatológico sequencial das lesões em ratos experimentalmente infectadosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleParacoccidioidomycosisParacoccidioides brasiliensiGranulomaExtracellular matrixCollagenProteoglycansParacoccidioidomicoseColágenoParacoccidioidesMatriz ExtracelularProteoglicanasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALParacoccidioidomycosis.pdfParacoccidioidomycosis.pdfapplication/pdf1826195https://www.arca.fiocruz.br/bitstream/icict/4070/1/Paracoccidioidomycosis.pdf8f843920d660eb82f35dafcd3681ce59MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/4070/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTParacoccidioidomycosis.pdf.txtParacoccidioidomycosis.pdf.txtExtracted texttext/plain33085https://www.arca.fiocruz.br/bitstream/icict/4070/5/Paracoccidioidomycosis.pdf.txt7d78b16b297f6f00692f16ecee07381bMD55THUMBNAILParacoccidioidomycosis.pdf.jpgParacoccidioidomycosis.pdf.jpgGenerated Thumbnailimage/jpeg1952https://www.arca.fiocruz.br/bitstream/icict/4070/4/Paracoccidioidomycosis.pdf.jpg5ae1b4aa006ffe6f39e5a99dc88f4d62MD54icict/40702018-04-06 08:58:04.035oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:58:04Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.en.fl_str_mv |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
dc.title.original.pt_BR.fl_str_mv |
Paracoccidioidomicose: estudo histopatológico sequencial das lesões em ratos experimentalmente infectados |
title |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
spellingShingle |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats Kerr, I. B. Paracoccidioidomycosis Paracoccidioides brasiliensi Granuloma Extracellular matrix Collagen Proteoglycans Paracoccidioidomicose Colágeno Paracoccidioides Matriz Extracelular Proteoglicanas |
title_short |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
title_full |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
title_fullStr |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
title_full_unstemmed |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
title_sort |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
author |
Kerr, I. B. |
author_facet |
Kerr, I. B. Araripe, J. R. Oliveira, P. C. Lenzi, Henrique L, |
author_role |
author |
author2 |
Araripe, J. R. Oliveira, P. C. Lenzi, Henrique L, |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Kerr, I. B. Araripe, J. R. Oliveira, P. C. Lenzi, Henrique L, |
dc.subject.en.pt_BR.fl_str_mv |
Paracoccidioidomycosis Paracoccidioides brasiliensi Granuloma Extracellular matrix Collagen Proteoglycans |
topic |
Paracoccidioidomycosis Paracoccidioides brasiliensi Granuloma Extracellular matrix Collagen Proteoglycans Paracoccidioidomicose Colágeno Paracoccidioides Matriz Extracelular Proteoglicanas |
dc.subject.decs.pt_BR.fl_str_mv |
Paracoccidioidomicose Colágeno Paracoccidioides Matriz Extracelular Proteoglicanas |
description |
Female albino rats were used for the sequential histopathological study of experimental paracoccidioidomycosis. The animals were inoculated intraperitoneally with a strain of Paracoccidioides brasiliensis in the yeast-like phase, and sacrificed at given intervals from 1 to 168 days after inoculation; each animal received an inoculum of 4 x 10^ cells in 0.8 ml of saline. The control group received saline containing scrapings of the culture medium. Tissue from the inoculation site was examined. The cellular population, the extracellular matrix, and the presence and characteristics of fungi were analysed in the inflammatory granulomatous process by light microscopy. The results allowed to separate the kinetic of the inflammatory response into three stages: 1) neutrophilic or macrophagic-neutrophilic; 2) pre-granulomatous; 3) granulomatous. Synthesis of the extracellular matrix began with the depositing of fibrin-like material, and increased gradually with deposits of collagen, proteoglycans, and glycoproteins. Parasites were present in all of the examined periods. Recurrences of the disease were clearly shown through the concurrence of recently-formed granulomas with older granulomas, implying that this type of granulomatous process does not eliminate the disease, nor is it able to limit fungal dissemination over a prolonged period of time. |
publishDate |
1988 |
dc.date.issued.fl_str_mv |
1988 |
dc.date.accessioned.fl_str_mv |
2012-05-15T15:17:48Z |
dc.date.available.fl_str_mv |
2012-05-15T15:17:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
KERR, I. B. et al. Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats. Revista do Instituto de Medicina Tropical, v.30, n.5, p.336-350, Sept./Oct.1988. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/4070 |
dc.identifier.issn.none.fl_str_mv |
0036-4665 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1590/S0036-46651988000500003 |
identifier_str_mv |
KERR, I. B. et al. Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats. Revista do Instituto de Medicina Tropical, v.30, n.5, p.336-350, Sept./Oct.1988. 0036-4665 |
url |
https://www.arca.fiocruz.br/handle/icict/4070 http://dx.doi.org/10.1590/S0036-46651988000500003 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Instituto de Medicina Tropical de São Paulo |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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