Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats
Autor(a) principal: | |
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Data de Publicação: | 1988 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28607 |
Resumo: | Female albino rats were used for the sequential histopathological study of experimental paracoccidioidomycosis. The animals were inoculated intraperitoneally with a strain of Paracoccidioides brasiliensis in the yeast-like phase, and sacrificed at given intervals from 1 to 168 days after inoculation; each animal received an inoculum of 4 x 10(6) cells in 0.8 ml of saline. The control group received saline containing scrapings of the culture medium. Tissue from the inoculation site was examined. The cellular population, the extracellular matrix, and the presence and characteristics of fungi were analysed in the inflammatory granulomatous process by light microscopy. The results allowed to separate the kinetic of the inflammatory response into three stages: 1) neutrophilic or macrophagic-neutrophilic; 2) pre-granulomatous; 3) granulomatous. Synthesis of the extracellular matrix began with the depositing of fibrin-like material, and increased gradually with deposits of collagen, proteoglycans, and glycoproteins. Parasites were present in all of the examined periods. Recurrences of the disease were clearly shown through the concurrence of recently-formed granulomas with older granulomas, implying that this type of granulomatous process does not eliminate the disease, nor is it able to limit fungal dissemination over a prolonged period of time. |
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Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats Paracoccidioidomicose: estudo histopatológico sequencial das lesões em ratos experimentalmente infectados ParacoccidioidomycosisParacoccidioides brasiliensisGranulomaExtracellular matrixCollagenProteoglycans Female albino rats were used for the sequential histopathological study of experimental paracoccidioidomycosis. The animals were inoculated intraperitoneally with a strain of Paracoccidioides brasiliensis in the yeast-like phase, and sacrificed at given intervals from 1 to 168 days after inoculation; each animal received an inoculum of 4 x 10(6) cells in 0.8 ml of saline. The control group received saline containing scrapings of the culture medium. Tissue from the inoculation site was examined. The cellular population, the extracellular matrix, and the presence and characteristics of fungi were analysed in the inflammatory granulomatous process by light microscopy. The results allowed to separate the kinetic of the inflammatory response into three stages: 1) neutrophilic or macrophagic-neutrophilic; 2) pre-granulomatous; 3) granulomatous. Synthesis of the extracellular matrix began with the depositing of fibrin-like material, and increased gradually with deposits of collagen, proteoglycans, and glycoproteins. Parasites were present in all of the examined periods. Recurrences of the disease were clearly shown through the concurrence of recently-formed granulomas with older granulomas, implying that this type of granulomatous process does not eliminate the disease, nor is it able to limit fungal dissemination over a prolonged period of time. Foram utilizados ratos albinos, fêmeas, para o estudo histopatológico sequencial da paracoccidioidomicose experimental. Os animais39 foram inoculados intraperitonealmente com uma cepa de Paracoccidioides brasiliensis na fase leveduriforme e sacrificados, em determinados intervalos, a partir de 1 a 168 dias pós-infecção; cada animal recebeu um inóculo de 4 x 10(6) células em 0,8 ml de salina. Os animais controles receberam salina contendo raspado do meio de cultura. Foram estudados tecidos correspondentesà área de inoculação. Analisou-se pela microscopia óptica o processo inflamatório granulomatoso em todo o seu conjunto, estudando a população celular, a matriz extracelular e a presença e características do fungo. Os resultados possibilitaram desmembrar a cinética da resposta inflamatória em três fases: 1) neutrofílica ou macrofágica-neutrofílica; 2) pré-granulomatosa; 3) granulomatosa. A síntese de matriz extracelular iniciou-se pela deposição de material fibrinóide, intensificando-se de modo gradativo com depósito de colágeno, de proteoglicanos e glicoproteínas. Os parasitos estavam presentes em todas as fases estudadas. Períodos de reativação da doença eram nitidamente evidenciados através da concomitância de granulomas recém-formados com granulomas mais antigos, indicando que o processo granulomatoso neste modelo não resolve a doença, nem tão pouco consegue limitar a disseminação do fungo por um período prolongado. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1988-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28607Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 No. 5 (1988); 336-350 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 Núm. 5 (1988); 336-350 Revista do Instituto de Medicina Tropical de São Paulo; v. 30 n. 5 (1988); 336-350 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28607/30460Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessKerr, I.B.Araripe, J.R.Oliveira, P.C.Lenzi, H.L.2012-07-02T01:06:35Zoai:revistas.usp.br:article/28607Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:20.898225Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats Paracoccidioidomicose: estudo histopatológico sequencial das lesões em ratos experimentalmente infectados |
title |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
spellingShingle |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats Kerr, I.B. Paracoccidioidomycosis Paracoccidioides brasiliensis Granuloma Extracellular matrix Collagen Proteoglycans |
title_short |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
title_full |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
title_fullStr |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
title_full_unstemmed |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
title_sort |
Paracoccidioidomycosis: a sequential histopathologic study of lesions in experimentally-infected rats |
author |
Kerr, I.B. |
author_facet |
Kerr, I.B. Araripe, J.R. Oliveira, P.C. Lenzi, H.L. |
author_role |
author |
author2 |
Araripe, J.R. Oliveira, P.C. Lenzi, H.L. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Kerr, I.B. Araripe, J.R. Oliveira, P.C. Lenzi, H.L. |
dc.subject.por.fl_str_mv |
Paracoccidioidomycosis Paracoccidioides brasiliensis Granuloma Extracellular matrix Collagen Proteoglycans |
topic |
Paracoccidioidomycosis Paracoccidioides brasiliensis Granuloma Extracellular matrix Collagen Proteoglycans |
description |
Female albino rats were used for the sequential histopathological study of experimental paracoccidioidomycosis. The animals were inoculated intraperitoneally with a strain of Paracoccidioides brasiliensis in the yeast-like phase, and sacrificed at given intervals from 1 to 168 days after inoculation; each animal received an inoculum of 4 x 10(6) cells in 0.8 ml of saline. The control group received saline containing scrapings of the culture medium. Tissue from the inoculation site was examined. The cellular population, the extracellular matrix, and the presence and characteristics of fungi were analysed in the inflammatory granulomatous process by light microscopy. The results allowed to separate the kinetic of the inflammatory response into three stages: 1) neutrophilic or macrophagic-neutrophilic; 2) pre-granulomatous; 3) granulomatous. Synthesis of the extracellular matrix began with the depositing of fibrin-like material, and increased gradually with deposits of collagen, proteoglycans, and glycoproteins. Parasites were present in all of the examined periods. Recurrences of the disease were clearly shown through the concurrence of recently-formed granulomas with older granulomas, implying that this type of granulomatous process does not eliminate the disease, nor is it able to limit fungal dissemination over a prolonged period of time. |
publishDate |
1988 |
dc.date.none.fl_str_mv |
1988-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28607 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28607 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28607/30460 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 No. 5 (1988); 336-350 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 30 Núm. 5 (1988); 336-350 Revista do Instituto de Medicina Tropical de São Paulo; v. 30 n. 5 (1988); 336-350 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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