A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/52882 https://doi.org/10.1007/s42770-022-00753-x |
Resumo: | Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil. |
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Conzentino, Marcelo S.Gonçalves, Ana C. A.Paula, Nigella M.Rego, Fabiane G. M.Zanette, Dalila LucíolaAoki, Mateus NóbregaNardin, Jeanine M.Huergo, Luciano Fernandes2022-05-25T18:59:15Z2022-05-25T18:59:15Z2022CONZENTINO, Marcelo S. et al. A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli. Brazilian Journal of Microbiology, p. 1–7, 2022.1678-4405https://www.arca.fiocruz.br/handle/icict/52882https://doi.org/10.1007/s42770-022-00753-xporSociedade Brasileira de MicrobiologiaCOVID-19SARS-CoV-2Magnetic beadsImmunoassayMagneticsReceptors, CoronavirusAntibodiesReceptors, IgGInmunoensayoReceptores de CoronavirusAnticuerposDosage immunologiqueMagnétismeAnticorpsRécepteurs du fragment Fc des IgGImunoensaioMagnetismoReceptores de CoronavírusAnticorposReceptores de IgGA magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coliinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Universidade Federal do Paraná. Programa de Pós-Graduação em Ciências Farmacêuticas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Hospital Erasto Gaertner. Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Immunological assays to detect SARS-CoV-2 Spike Receptor Binding Domain (RBD) antigen seroconversion in humans are important tools to monitor the levels of protecting antibodies in the population in response to infection and/or immunization. Here we describe a simple, low cost, and high throughput Ni2+ magnetic bead immunoassay to detect human IgG reactive to Spike S1 RBD Receptor Binding Domain produced in Escherichia coli. A 6xHis-tagged Spike S1 RBD was expressed in E. coli and purifed by afnity chromatography. The protein was mobilized on the surface of Ni2+ magnetic beads and used to investigate the presence of reactive IgG in the serum obtained from pre-pandemic and COVID-19 confrmed cases. The method was validated with a cohort of 290 samples and an area under the receiver operating characteristic curve of 0.94 was obtained. The method was operated with>82% sensitivity at 98% specifcity and was also able to track human IgG raised in response to vaccination with Comirnaty at>85% sensitivity. The IgG signal obtained with the described method was well-correlated with the signal obtained when pre fusion Spike produced in HEK cell lines was used as antigen. This novel low-cost and high throughput immunoassay may act as an important tool to investigate protecting IgG antibodies against SARS-CoV-2 in the human population.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/52882/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALConzentino_Marcelo_S-A_magnetic_beadOK.pdfConzentino_Marcelo_S-A_magnetic_beadOK.pdfapplication/pdf1067232https://www.arca.fiocruz.br/bitstream/icict/52882/2/Conzentino_Marcelo_S-A_magnetic_beadOK.pdf700b47a607f6729843e3d92155974286MD52icict/528822022-05-25 15:59:15.658oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-05-25T18:59:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli |
title |
A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli |
spellingShingle |
A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli Conzentino, Marcelo S. COVID-19 SARS-CoV-2 Magnetic beads Immunoassay Magnetics Receptors, Coronavirus Antibodies Receptors, IgG Inmunoensayo Receptores de Coronavirus Anticuerpos Dosage immunologique Magnétisme Anticorps Récepteurs du fragment Fc des IgG Imunoensaio Magnetismo Receptores de Coronavírus Anticorpos Receptores de IgG |
title_short |
A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli |
title_full |
A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli |
title_fullStr |
A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli |
title_full_unstemmed |
A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli |
title_sort |
A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli |
author |
Conzentino, Marcelo S. |
author_facet |
Conzentino, Marcelo S. Gonçalves, Ana C. A. Paula, Nigella M. Rego, Fabiane G. M. Zanette, Dalila Lucíola Aoki, Mateus Nóbrega Nardin, Jeanine M. Huergo, Luciano Fernandes |
author_role |
author |
author2 |
Gonçalves, Ana C. A. Paula, Nigella M. Rego, Fabiane G. M. Zanette, Dalila Lucíola Aoki, Mateus Nóbrega Nardin, Jeanine M. Huergo, Luciano Fernandes |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Conzentino, Marcelo S. Gonçalves, Ana C. A. Paula, Nigella M. Rego, Fabiane G. M. Zanette, Dalila Lucíola Aoki, Mateus Nóbrega Nardin, Jeanine M. Huergo, Luciano Fernandes |
dc.subject.other.pt_BR.fl_str_mv |
COVID-19 SARS-CoV-2 Magnetic beads |
topic |
COVID-19 SARS-CoV-2 Magnetic beads Immunoassay Magnetics Receptors, Coronavirus Antibodies Receptors, IgG Inmunoensayo Receptores de Coronavirus Anticuerpos Dosage immunologique Magnétisme Anticorps Récepteurs du fragment Fc des IgG Imunoensaio Magnetismo Receptores de Coronavírus Anticorpos Receptores de IgG |
dc.subject.en.pt_BR.fl_str_mv |
Immunoassay Magnetics Receptors, Coronavirus Antibodies Receptors, IgG |
dc.subject.es.pt_BR.fl_str_mv |
Inmunoensayo Receptores de Coronavirus Anticuerpos |
dc.subject.fr.pt_BR.fl_str_mv |
Dosage immunologique Magnétisme Anticorps Récepteurs du fragment Fc des IgG |
dc.subject.decs.pt_BR.fl_str_mv |
Imunoensaio Magnetismo Receptores de Coronavírus Anticorpos Receptores de IgG |
description |
Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-05-25T18:59:15Z |
dc.date.available.fl_str_mv |
2022-05-25T18:59:15Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CONZENTINO, Marcelo S. et al. A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli. Brazilian Journal of Microbiology, p. 1–7, 2022. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/52882 |
dc.identifier.issn.pt_BR.fl_str_mv |
1678-4405 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1007/s42770-022-00753-x |
identifier_str_mv |
CONZENTINO, Marcelo S. et al. A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS‑CoV‑2 Spike S1 RBD produced in Escherichia coli. Brazilian Journal of Microbiology, p. 1–7, 2022. 1678-4405 |
url |
https://www.arca.fiocruz.br/handle/icict/52882 https://doi.org/10.1007/s42770-022-00753-x |
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por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/52882/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/52882/2/Conzentino_Marcelo_S-A_magnetic_beadOK.pdf |
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repositorio.arca@fiocruz.br |
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