Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?

Detalhes bibliográficos
Autor(a) principal: Krawczak, Felipe da Silva
Data de Publicação: 2015
Outros Autores: Reis, Ilka Afonso, Silveira, Julia Angélica da, Avelar, Daniel Moreira, Marcelino, Andreza Pain, Werneck, Guilherme Loureiro, Labruna, Marcelo Bahia, Paz, Gustavo Fontes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12607
Resumo: Universidade de São Paulo. Departamento de Medicina Veterinária Preventiva e Saúde Animal. Faculdade de Medicina Veterinária e Zootecnia. São Paulo, SP, Brasil
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spelling Krawczak, Felipe da SilvaReis, Ilka AfonsoSilveira, Julia Angélica daAvelar, Daniel MoreiraMarcelino, Andreza PainWerneck, Guilherme LoureiroLabruna, Marcelo BahiaPaz, Gustavo Fontes2016-01-25T17:21:46Z2016-01-25T17:21:46Z2015KRAWCZAK, Felipe da Silva et al. Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods? Rev. Soc. Bras. Med. Trop., vol.48 n.1, p. 64-68, 20150037-8682https://www.arca.fiocruz.br/handle/icict/1260710.1590/0037-8682-0291-2014engSociedade Brasileira de Medicina TropicalLeishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Departamento de Medicina Veterinária Preventiva e Saúde Animal. Faculdade de Medicina Veterinária e Zootecnia. São Paulo, SP, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Exatas. Departamento de Estatística. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilFundação Ezequiel Dias. Divisão de Epidemiologia e Controle de Doenças. Serviço de Doenças Parasitárias. Belo Horizonte, MG, BrasilFundação Ezequiel Dias. Divisão de Epidemiologia e Controle de Doenças. Serviço de Doenças Parasitárias. Belo Horizonte, MG, BrasilUniversidade do Estado do Rio de Janeiro. Instituto de Medicina Social. Departamento de Epidemiologia. Rio de Janeiro, RJ, BrasilUniversidade de São Paulo. Departamento de Medicina Veterinária Preventiva e Saúde Animal. Faculdade de Medicina Veterinária e Zootecnia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG Brasil.INTRODUCTION: The present study was designed to assess the occurrence of co-infection or cross-reaction in the serological techniques used for detecting the anti-Leishmania spp., -Babesia canis vogeli and -Ehrlichia canis antibodies in urban dogs from an area endemic to these parasites. METHODS: The serum samples from dogs were tested for the Babesia canis vogeli strain Belo Horizonte antigen and Ehrlichia canis strain São Paulo by immunofluorescence antibody test (IFAT) and by anti-Leishmania immunoglobulin G (IgG) antibody detection to assess Leishmania infection. We used the following four commercial kits for canine visceral leishmaniasis: ELISA, IFAT, Dual Path Platform (DPP) (Bio Manguinhos(r)/FIOCRUZ/MS) and a rK39 RDT (Kalazar Detect Canine Rapid Test; Inbios). RESULTS: Of 96 serum samples submitted to serological assays, 4 (4.2%) were positive for Leishmania as determined by ELISA; 12 (12.5%), by IFAT; 14 (14.6%) by rK39 RDT; and 20 (20.8%), by DPP. Antibodies against Ehrlichia and Babesia were detected in 23/96 (23.9%) and 30/96 (31.2%) samples, respectively. No significant association was identified between the results of tests for detecting Babesia or Ehrlichia and those for detecting Leishmania (p-value>0.05). CONCLUSIONS: In the present study, we demonstrated co-infection with Ehrlichia or Babesia and Leishmania in dogs from Minas Gerais (Brazil); we also found that the serological tests that were used did not cross-react.Immunochromatographic testEnzyme-linked immunosorbent assayImmunofluorescence antibody testCanine visceral leishmaniasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12607/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLeishmania, Babesia and Ehrlichia in urban pet dogs.pdfLeishmania, Babesia and Ehrlichia in urban pet dogs.pdfapplication/pdf4849633https://www.arca.fiocruz.br/bitstream/icict/12607/2/Leishmania%2c%20Babesia%20and%20Ehrlichia%20in%20urban%20pet%20dogs.pdf4200cad3c205e69d326679ef177238e4MD52TEXTLeishmania, Babesia and Ehrlichia in urban pet 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dc.title.pt_BR.fl_str_mv Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
title Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
spellingShingle Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
Krawczak, Felipe da Silva
Immunochromatographic test
Enzyme-linked immunosorbent assay
Immunofluorescence antibody test
Canine visceral leishmaniasis
title_short Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
title_full Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
title_fullStr Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
title_full_unstemmed Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
title_sort Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?
author Krawczak, Felipe da Silva
author_facet Krawczak, Felipe da Silva
Reis, Ilka Afonso
Silveira, Julia Angélica da
Avelar, Daniel Moreira
Marcelino, Andreza Pain
Werneck, Guilherme Loureiro
Labruna, Marcelo Bahia
Paz, Gustavo Fontes
author_role author
author2 Reis, Ilka Afonso
Silveira, Julia Angélica da
Avelar, Daniel Moreira
Marcelino, Andreza Pain
Werneck, Guilherme Loureiro
Labruna, Marcelo Bahia
Paz, Gustavo Fontes
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Krawczak, Felipe da Silva
Reis, Ilka Afonso
Silveira, Julia Angélica da
Avelar, Daniel Moreira
Marcelino, Andreza Pain
Werneck, Guilherme Loureiro
Labruna, Marcelo Bahia
Paz, Gustavo Fontes
dc.subject.en.pt_BR.fl_str_mv Immunochromatographic test
Enzyme-linked immunosorbent assay
Immunofluorescence antibody test
Canine visceral leishmaniasis
topic Immunochromatographic test
Enzyme-linked immunosorbent assay
Immunofluorescence antibody test
Canine visceral leishmaniasis
description Universidade de São Paulo. Departamento de Medicina Veterinária Preventiva e Saúde Animal. Faculdade de Medicina Veterinária e Zootecnia. São Paulo, SP, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-25T17:21:46Z
dc.date.available.fl_str_mv 2016-01-25T17:21:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv KRAWCZAK, Felipe da Silva et al. Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods? Rev. Soc. Bras. Med. Trop., vol.48 n.1, p. 64-68, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12607
dc.identifier.issn.none.fl_str_mv 0037-8682
dc.identifier.doi.none.fl_str_mv 10.1590/0037-8682-0291-2014
identifier_str_mv KRAWCZAK, Felipe da Silva et al. Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods? Rev. Soc. Bras. Med. Trop., vol.48 n.1, p. 64-68, 2015
0037-8682
10.1590/0037-8682-0291-2014
url https://www.arca.fiocruz.br/handle/icict/12607
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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