Molecular characterization of Candida spp. isolates from patients with bloodstream infections

Detalhes bibliográficos
Autor(a) principal: Xavier, Paula Cristhina Niz
Data de Publicação: 2013
Outros Autores: Chang, Marilene Rodrigues, Paula, Claudete Rodrigues, Matsumoto, Flávia Emi, Asensi, Marise Dutra, Matos, Maria de Fátima Cepa, Andreotti, Renato
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11857
Resumo: Universidade Federal do Mato Grosso do Sul. Programa de Pós-Graduação. Campo Grande, MS, Brasil.
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spelling Xavier, Paula Cristhina NizChang, Marilene RodriguesPaula, Claudete RodriguesMatsumoto, Flávia EmiAsensi, Marise DutraMatos, Maria de Fátima CepaAndreotti, Renato2015-10-02T12:45:00Z2015-10-02T12:45:00Z2013XAVIER, Paula Cristhina Niz; et al. Molecular characterization of Candida spp. isolates from patients with bloodstream infections. Revista da Sociedade Brasileira de Medicina Tropical, v.46, n.6, p.786-787, Nov-Dec, 2013.0037-8682https://www.arca.fiocruz.br/handle/icict/1185710.1590/0037-8682-1718-2013porSociedade Brasileira de Medicina TropicalMolecular characterization of Candida spp. isolates from patients with bloodstream infectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Mato Grosso do Sul. Programa de Pós-Graduação. Campo Grande, MS, Brasil.Universidade Federal do Mato Grosso do Sul. Departamento de Farmácia e Bioquímica. Campo Grande, MS, Brasil.Universidade de São Paulo. Instituto de Ciências Biológicas. Departamento de Microbiologia. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Biológicas. Departamento de Microbiologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Enterobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal do Mato Grosso do Sul. Departamento de Farmácia e Bioquímica. Campo Grande, MS, Brasil.Empresa Brasileira de Pesquisa Agropecuária. Gado de Corte. Campo Grande, MS, Brasil.The aim of this study was to conduct an epidemiological study comparing the genetic similarity of yeasts isolated from blood cultures. Methods: Random amplifi cation of polymorphic DNA (RAPD) techniques were used for the Candida samples obtained from patients at the Hospital Universitário da Universidade Federal do Mato Grosso do Sul (HU/UFMS) in Campo Grande, State of Mato Grosso do Sul, Brazil, from 1998-2000. Results: The most frequently isolated species was Candida albicans (45.8%). DNA amplifi cation from genomic yeast isolates indicated a genetic similarity of over 90%. Conclusions: The RAPD profi les obtained were able to differentiate between the isolated Candida species, thereby suggesting that the method might be useful in epidemiological studies.CandidaPolymerase chain reactionRandom amplification of polymorphic DNAReação em Cadeia da Polimerase Via Transcriptase ReversaCandidaTécnica de Amplificação ao Acaso de DNA Polimórficoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11857/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmarise_asensi_etal_IOC_2013.pdfapplication/pdf921450https://www.arca.fiocruz.br/bitstream/icict/11857/2/marise_asensi_etal_IOC_2013.pdf3445561a166f29a9a5b12a89bbd78ad4MD52TEXTmarise_asensi_etal_IOC_2013.pdf.txtmarise_asensi_etal_IOC_2013.pdf.txtExtracted texttext/plain8363https://www.arca.fiocruz.br/bitstream/icict/11857/3/marise_asensi_etal_IOC_2013.pdf.txtc3b4a677b52455504568325ed754e3caMD53icict/118572023-09-05 10:26:09.648oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:26:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Molecular characterization of Candida spp. isolates from patients with bloodstream infections
title Molecular characterization of Candida spp. isolates from patients with bloodstream infections
spellingShingle Molecular characterization of Candida spp. isolates from patients with bloodstream infections
Xavier, Paula Cristhina Niz
Candida
Polymerase chain reaction
Random amplification of polymorphic DNA
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Candida
Técnica de Amplificação ao Acaso de DNA Polimórfico
title_short Molecular characterization of Candida spp. isolates from patients with bloodstream infections
title_full Molecular characterization of Candida spp. isolates from patients with bloodstream infections
title_fullStr Molecular characterization of Candida spp. isolates from patients with bloodstream infections
title_full_unstemmed Molecular characterization of Candida spp. isolates from patients with bloodstream infections
title_sort Molecular characterization of Candida spp. isolates from patients with bloodstream infections
author Xavier, Paula Cristhina Niz
author_facet Xavier, Paula Cristhina Niz
Chang, Marilene Rodrigues
Paula, Claudete Rodrigues
Matsumoto, Flávia Emi
Asensi, Marise Dutra
Matos, Maria de Fátima Cepa
Andreotti, Renato
author_role author
author2 Chang, Marilene Rodrigues
Paula, Claudete Rodrigues
Matsumoto, Flávia Emi
Asensi, Marise Dutra
Matos, Maria de Fátima Cepa
Andreotti, Renato
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Xavier, Paula Cristhina Niz
Chang, Marilene Rodrigues
Paula, Claudete Rodrigues
Matsumoto, Flávia Emi
Asensi, Marise Dutra
Matos, Maria de Fátima Cepa
Andreotti, Renato
dc.subject.en.pt_BR.fl_str_mv Candida
Polymerase chain reaction
Random amplification of polymorphic DNA
topic Candida
Polymerase chain reaction
Random amplification of polymorphic DNA
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Candida
Técnica de Amplificação ao Acaso de DNA Polimórfico
dc.subject.decs.pt_BR.fl_str_mv Reação em Cadeia da Polimerase Via Transcriptase Reversa
Candida
Técnica de Amplificação ao Acaso de DNA Polimórfico
description Universidade Federal do Mato Grosso do Sul. Programa de Pós-Graduação. Campo Grande, MS, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-10-02T12:45:00Z
dc.date.available.fl_str_mv 2015-10-02T12:45:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv XAVIER, Paula Cristhina Niz; et al. Molecular characterization of Candida spp. isolates from patients with bloodstream infections. Revista da Sociedade Brasileira de Medicina Tropical, v.46, n.6, p.786-787, Nov-Dec, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11857
dc.identifier.issn.pt_BR.fl_str_mv 0037-8682
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/0037-8682-1718-2013
identifier_str_mv XAVIER, Paula Cristhina Niz; et al. Molecular characterization of Candida spp. isolates from patients with bloodstream infections. Revista da Sociedade Brasileira de Medicina Tropical, v.46, n.6, p.786-787, Nov-Dec, 2013.
0037-8682
10.1590/0037-8682-1718-2013
url https://www.arca.fiocruz.br/handle/icict/11857
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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