Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA

Detalhes bibliográficos
Autor(a) principal: Pinto,Patrícia M
Data de Publicação: 2004
Outros Autores: Resende,Maria A, Koga-Ito,Cristiane Y, Tendler,Miriam
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000200006
Resumo: The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi.
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spelling Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNApolymerase chain reactionrandom amplified polymorphic DNAcandidosisCandida sppThe patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi.Instituto Oswaldo Cruz, Ministério da Saúde2004-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000200006Memórias do Instituto Oswaldo Cruz v.99 n.2 2004reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762004000200006info:eu-repo/semantics/openAccessPinto,Patrícia MResende,Maria AKoga-Ito,Cristiane YTendler,Miriameng2020-04-25T17:49:13Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:30.462Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
title Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
spellingShingle Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
Pinto,Patrícia M
polymerase chain reaction
random amplified polymorphic DNA
candidosis
Candida spp
title_short Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
title_full Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
title_fullStr Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
title_full_unstemmed Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
title_sort Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
author Pinto,Patrícia M
author_facet Pinto,Patrícia M
Resende,Maria A
Koga-Ito,Cristiane Y
Tendler,Miriam
author_role author
author2 Resende,Maria A
Koga-Ito,Cristiane Y
Tendler,Miriam
author2_role author
author
author
dc.contributor.author.fl_str_mv Pinto,Patrícia M
Resende,Maria A
Koga-Ito,Cristiane Y
Tendler,Miriam
dc.subject.por.fl_str_mv polymerase chain reaction
random amplified polymorphic DNA
candidosis
Candida spp
topic polymerase chain reaction
random amplified polymorphic DNA
candidosis
Candida spp
dc.description.none.fl_txt_mv The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi.
description The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi.
publishDate 2004
dc.date.none.fl_str_mv 2004-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000200006
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762004000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.99 n.2 2004
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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