Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762004000200006 http://hdl.handle.net/11449/22711 |
Resumo: | The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi. |
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Repositório Institucional da UNESP |
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Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNApolymerase chain reactionrandom amplified polymorphic DNAcandidosisCandida sppThe patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi.Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de MicrobiologiaInstituto Oswaldo Cruz-Fiocruz Departamento de HelmintologiaUnesp Faculdade de Odontologia de São José dos Campos Departamento de Biociências e Diagnóstico BucalUnesp Faculdade de Odontologia de São José dos Campos Departamento de Biociências e Diagnóstico BucalInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Federal de Minas Gerais (UFMG)Instituto Oswaldo Cruz-Fiocruz Departamento de HelmintologiaUniversidade Estadual Paulista (Unesp)Pinto, Patrícia MResende, Maria AKoga-Ito, Cristiane Y [UNESP]Tendler, Miriam2014-05-20T14:04:45Z2014-05-20T14:04:45Z2004-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article147-152application/pdfhttp://dx.doi.org/10.1590/S0074-02762004000200006Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 2, p. 147-152, 2004.0074-0276http://hdl.handle.net/11449/2271110.1590/S0074-02762004000200006S0074-02762004000200006S0074-02762004000200006.pdf65435631614034210000-0002-2416-2173SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2023-11-10T06:15:49Zoai:repositorio.unesp.br:11449/22711Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:20:57.356298Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA |
title |
Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA |
spellingShingle |
Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA Pinto, Patrícia M polymerase chain reaction random amplified polymorphic DNA candidosis Candida spp |
title_short |
Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA |
title_full |
Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA |
title_fullStr |
Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA |
title_full_unstemmed |
Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA |
title_sort |
Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA |
author |
Pinto, Patrícia M |
author_facet |
Pinto, Patrícia M Resende, Maria A Koga-Ito, Cristiane Y [UNESP] Tendler, Miriam |
author_role |
author |
author2 |
Resende, Maria A Koga-Ito, Cristiane Y [UNESP] Tendler, Miriam |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG) Instituto Oswaldo Cruz-Fiocruz Departamento de Helmintologia Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pinto, Patrícia M Resende, Maria A Koga-Ito, Cristiane Y [UNESP] Tendler, Miriam |
dc.subject.por.fl_str_mv |
polymerase chain reaction random amplified polymorphic DNA candidosis Candida spp |
topic |
polymerase chain reaction random amplified polymorphic DNA candidosis Candida spp |
description |
The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-03-01 2014-05-20T14:04:45Z 2014-05-20T14:04:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762004000200006 Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 2, p. 147-152, 2004. 0074-0276 http://hdl.handle.net/11449/22711 10.1590/S0074-02762004000200006 S0074-02762004000200006 S0074-02762004000200006.pdf 6543563161403421 0000-0002-2416-2173 |
url |
http://dx.doi.org/10.1590/S0074-02762004000200006 http://hdl.handle.net/11449/22711 |
identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 2, p. 147-152, 2004. 0074-0276 10.1590/S0074-02762004000200006 S0074-02762004000200006 S0074-02762004000200006.pdf 6543563161403421 0000-0002-2416-2173 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz 2.833 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
147-152 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128795594457088 |