Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA

Detalhes bibliográficos
Autor(a) principal: Pinto, Patrícia M
Data de Publicação: 2004
Outros Autores: Resende, Maria A, Koga-Ito, Cristiane Y [UNESP], Tendler, Miriam
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762004000200006
http://hdl.handle.net/11449/22711
Resumo: The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi.
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spelling Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNApolymerase chain reactionrandom amplified polymorphic DNAcandidosisCandida sppThe patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi.Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de MicrobiologiaInstituto Oswaldo Cruz-Fiocruz Departamento de HelmintologiaUnesp Faculdade de Odontologia de São José dos Campos Departamento de Biociências e Diagnóstico BucalUnesp Faculdade de Odontologia de São José dos Campos Departamento de Biociências e Diagnóstico BucalInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Federal de Minas Gerais (UFMG)Instituto Oswaldo Cruz-Fiocruz Departamento de HelmintologiaUniversidade Estadual Paulista (Unesp)Pinto, Patrícia MResende, Maria AKoga-Ito, Cristiane Y [UNESP]Tendler, Miriam2014-05-20T14:04:45Z2014-05-20T14:04:45Z2004-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article147-152application/pdfhttp://dx.doi.org/10.1590/S0074-02762004000200006Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 2, p. 147-152, 2004.0074-0276http://hdl.handle.net/11449/2271110.1590/S0074-02762004000200006S0074-02762004000200006S0074-02762004000200006.pdf65435631614034210000-0002-2416-2173SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2023-11-10T06:15:49Zoai:repositorio.unesp.br:11449/22711Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:20:57.356298Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
title Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
spellingShingle Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
Pinto, Patrícia M
polymerase chain reaction
random amplified polymorphic DNA
candidosis
Candida spp
title_short Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
title_full Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
title_fullStr Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
title_full_unstemmed Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
title_sort Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
author Pinto, Patrícia M
author_facet Pinto, Patrícia M
Resende, Maria A
Koga-Ito, Cristiane Y [UNESP]
Tendler, Miriam
author_role author
author2 Resende, Maria A
Koga-Ito, Cristiane Y [UNESP]
Tendler, Miriam
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Minas Gerais (UFMG)
Instituto Oswaldo Cruz-Fiocruz Departamento de Helmintologia
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Pinto, Patrícia M
Resende, Maria A
Koga-Ito, Cristiane Y [UNESP]
Tendler, Miriam
dc.subject.por.fl_str_mv polymerase chain reaction
random amplified polymorphic DNA
candidosis
Candida spp
topic polymerase chain reaction
random amplified polymorphic DNA
candidosis
Candida spp
description The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi.
publishDate 2004
dc.date.none.fl_str_mv 2004-03-01
2014-05-20T14:04:45Z
2014-05-20T14:04:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762004000200006
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 2, p. 147-152, 2004.
0074-0276
http://hdl.handle.net/11449/22711
10.1590/S0074-02762004000200006
S0074-02762004000200006
S0074-02762004000200006.pdf
6543563161403421
0000-0002-2416-2173
url http://dx.doi.org/10.1590/S0074-02762004000200006
http://hdl.handle.net/11449/22711
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 2, p. 147-152, 2004.
0074-0276
10.1590/S0074-02762004000200006
S0074-02762004000200006
S0074-02762004000200006.pdf
6543563161403421
0000-0002-2416-2173
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv 147-152
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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