Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14237 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. |
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Vieira, Cecilia S.Mattos, Débora P.Waniek, Peter J.Santangelo, Jayme M.Figueiredo, Marcela B.Gumiel, MarciaMota, Fabio F. daCastro, Daniele P.Garcia, Eloi S.Azambuja, Patricia2016-05-11T13:05:36Z2016-05-11T13:05:36Z2015VIEIRA, Cecilia S. et al. Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population. Parasites & Vectors, v.8, n.135, 13p, 2015.https://www.arca.fiocruz.br/handle/icict/1423710.1186/s13071-015-0736-21756-3305engBioMed CentralRhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota populationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro (UFRRJ). Instituto de Florestas. Departamento de Ciências Ambientais. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM). Departamento de Entomologia Molecular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM). Departamento de Entomologia Molecular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM). Departamento de Entomologia Molecular. Rio de Janeiro, RJ. Brasil.Background: Trypanosoma rangeli is a protozoan that infects a variety of mammalian hosts, including humans. Its main insect vector is Rhodnius prolixus and is found in several Latin American countries. The R. prolixus vector competence depends on the T. rangeli strain and the molecular interactions, as well as the insect’s immune responses in the gut and haemocoel. This work focuses on the modulation of the humoral immune responses of the midgut of R. prolixus infected with T. rangeli Macias strain, considering the influence of the parasite on the intestinal microbiota. Methods: The population density of T. rangeli Macias strain was analysed in different R. prolixus midgut compartments in long and short-term experiments. Cultivable and non-cultivable midgut bacteria were investigated by colony forming unit (CFU) assays and by 454 pyrosequencing of the 16S rRNA gene, respectively. The modulation of R. prolixus immune responses was studied by analysis of the antimicrobial activity in vitro against different bacteria using turbidimetric tests, the abundance of mRNAs encoding antimicrobial peptides (AMPs) defensin (DefA, DefB, DefC), prolixicin (Prol) and lysozymes (LysA, LysB) by RT-PCR and analysis of the phenoloxidase (PO) activity. Results: Our results showed that T. rangeli successfully colonized R. prolixus midgut altering the microbiota population and the immune responses as follows: 1 - reduced cultivable midgut bacteria; 2 - decreased the number of sequences of the Enterococcaceae but increased those of the Burkholderiaceae family; the families Nocardiaceae, Enterobacteriaceae and Mycobacteriaceae encountered in control and infected insects remained the same; 3 - enhanced midgut antibacterial activities against Serratia marcescens and Staphylococcus aureus; 4 - downregulated LysB and Prol mRNA levels; altered DefB, DefC and LysA depending on the infection (short and longterm); 5 - decreased PO activity. Conclusion: Our findings suggest that T. rangeli Macias strain modulates R. prolixus immune system and modifies the natural microbiota composition.Rhodnius prolixusTrypanosoma rangeliImmune systemProphenoloxidaseAntimicrobial peptideSistema ImunológicoTrypanosoma rangeliinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14237/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALcecilia_vieira_etal_IOC_2015.pdfcecilia_vieira_etal_IOC_2015.pdfapplication/pdf1447403https://www.arca.fiocruz.br/bitstream/icict/14237/2/cecilia_vieira_etal_IOC_2015.pdf9b50113b9471461d6d9b11f81121be77MD52TEXTcecilia_vieira_etal_IOC_2015.pdf.txtcecilia_vieira_etal_IOC_2015.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population |
| title |
Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population |
| spellingShingle |
Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population Vieira, Cecilia S. Rhodnius prolixus Trypanosoma rangeli Immune system Prophenoloxidase Antimicrobial peptide Sistema Imunológico Trypanosoma rangeli |
| title_short |
Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population |
| title_full |
Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population |
| title_fullStr |
Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population |
| title_full_unstemmed |
Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population |
| title_sort |
Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population |
| author |
Vieira, Cecilia S. |
| author_facet |
Vieira, Cecilia S. Mattos, Débora P. Waniek, Peter J. Santangelo, Jayme M. Figueiredo, Marcela B. Gumiel, Marcia Mota, Fabio F. da Castro, Daniele P. Garcia, Eloi S. Azambuja, Patricia |
| author_role |
author |
| author2 |
Mattos, Débora P. Waniek, Peter J. Santangelo, Jayme M. Figueiredo, Marcela B. Gumiel, Marcia Mota, Fabio F. da Castro, Daniele P. Garcia, Eloi S. Azambuja, Patricia |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vieira, Cecilia S. Mattos, Débora P. Waniek, Peter J. Santangelo, Jayme M. Figueiredo, Marcela B. Gumiel, Marcia Mota, Fabio F. da Castro, Daniele P. Garcia, Eloi S. Azambuja, Patricia |
| dc.subject.en.pt_BR.fl_str_mv |
Rhodnius prolixus Trypanosoma rangeli Immune system Prophenoloxidase Antimicrobial peptide |
| topic |
Rhodnius prolixus Trypanosoma rangeli Immune system Prophenoloxidase Antimicrobial peptide Sistema Imunológico Trypanosoma rangeli |
| dc.subject.decs.pt_BR.fl_str_mv |
Sistema Imunológico Trypanosoma rangeli |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. |
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2015 |
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2015 |
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2016-05-11T13:05:36Z |
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2016-05-11T13:05:36Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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VIEIRA, Cecilia S. et al. Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population. Parasites & Vectors, v.8, n.135, 13p, 2015. |
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https://www.arca.fiocruz.br/handle/icict/14237 |
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10.1186/s13071-015-0736-2 |
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1756-3305 |
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VIEIRA, Cecilia S. et al. Rhodnius prolixus interaction with Trypanosoma rangeli: modulation of the immune system and microbiota population. Parasites & Vectors, v.8, n.135, 13p, 2015. 10.1186/s13071-015-0736-2 1756-3305 |
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BioMed Central |
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BioMed Central |
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