HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)

Detalhes bibliográficos
Autor(a) principal: Freitas, Maria Audilene de
Data de Publicação: 2022
Outros Autores: Cruz, Rafael Pereira da, Santos, Antonia Thassya Lucas dos, Bezerra, José Weverton Almeida, Machado, Antonio Júdson Targino, Santos, Joycy Francely Sampaio dos, Rocha, Janaina Esmeraldo, Boligon, Aline Augusti, Bezerra, Camila Fonseca, Freitas, Thiago Sampaio de, Silva, Maria Karollyna do Nascimento, Mendonça, Ana Cleide Alcântara Morais, Costa, José Galberto Martins da, Coutinho, Henrique Douglas Melo, Cunha, Francisco Assis Bezerra da, Ribeiro Filho, Jaime, Braga, Maria Flaviana Bezerra Morais
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51683
Resumo: Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Federal de Santa Maria. Santa Maria, RS, Brasil. Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil.
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spelling Freitas, Maria Audilene deCruz, Rafael Pereira daSantos, Antonia Thassya Lucas dosBezerra, José Weverton AlmeidaMachado, Antonio Júdson TarginoSantos, Joycy Francely Sampaio dosRocha, Janaina EsmeraldoBoligon, Aline AugustiBezerra, Camila FonsecaFreitas, Thiago Sampaio deSilva, Maria Karollyna do NascimentoMendonça, Ana Cleide Alcântara MoraisCosta, José Galberto Martins daCoutinho, Henrique Douglas MeloCunha, Francisco Assis Bezerra daRibeiro Filho, JaimeBraga, Maria Flaviana Bezerra Morais2022-03-11T13:40:54Z2022-03-11T13:40:54Z2022Freitas, Maria Audilene de et al. HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae). 3 Biotech, v. 12, n. 1, p. 1-16, 2022.2190-5738https://www.arca.fiocruz.br/handle/icict/51683doi.org/10.1007/s13205-022-03126-1Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Federal de Santa Maria. Santa Maria, RS, Brasil. Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil.Fundação Cearense de Apoio ao Desenvolvimento (FUNCAP). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Financiadora de Estudos e Projetos (FINEP)."Múltipla ver em Notas"Spondias mombin is used in the folk medicine for the treatment of diarrhea and dysentery, indicating that extracts obtained from this species may present pharmacological activities against pathogenic microorganisms. The purpose of this work was to investigate the chemical composition and evaluate the antimicrobial activity of extracts obtained from the leaves (aqueous) and bark (hydroethanolic) of S. mombin both as single treatments and in combination with conventional drugs. Following a qualitative chemical prospection, the extracts were analyzed by HPLC–DAD. The antimicrobial activities were evaluated by microdilution. The combined activity of drugs and extracts was verified by adding a subinhibitory concentration of the extract in the presence of variable drug concentrations. The Minimum Fungicidal Concentration (MFC) was determined by a subculture of the microdilution test, while the effect of the in vitro treatments on morphological transition was analyzed by subculture in moist chambers. While the qualitative analysis detected the presence of phenols and flavonoids, the HPLC analysis identified quercetin, caffeic acid, and catechin as major components in the leaf extract, whereas kaempferol and quercetin were found as major compounds in the bark extract. The extracts showed effective antibacterial activities only against the Gram-negative strains. With regard to the combined activity, the leaf extract potentiated the action of gentamicin and imipenem (against Staphylococcus aureus), while the bark extract potentiated the effect of norfloxacin (against S. aureus), imipenem (against Escherichia coli), and norfloxacin (against Pseudomonas aeruginosa). A more significant antifungal (fungistatic) effect was achieved with the bark extract (even though at high concentrations), which further enhanced the activity of fluconazole. The extracts also inhibited the emission of filaments by Candida albicans and Candida tropicalis. Together, these findings suggest that that the extract constituents may act by favoring the permeability of microbial cells to conventional drugs, as well as by affecting virulence mechanisms in Candida strains.engSpringerCompostos fitoquímicosAntibacterianosAntifúngicosPhytochemicalsAnti-Bacterial agentsAntifungal agentsFitoquímicosAntibacterianosAntifúngicosComposés phytochimiquesAntibactériensAntifongiquesHPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/51683/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFreitas M A - HPLC-DAD analysis.pdfFreitas M A - HPLC-DAD analysis.pdfapplication/pdf2238132https://www.arca.fiocruz.br/bitstream/icict/51683/2/Freitas%20M%20A%20-%20HPLC-DAD%20analysis.pdf1140e30f7e00cad294f8579911dbba1dMD52icict/516832023-03-15 14:33:25.272oai:www.arca.fiocruz.br:icict/51683Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)
title HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)
spellingShingle HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)
Freitas, Maria Audilene de
Compostos fitoquímicos
Antibacterianos
Antifúngicos
Phytochemicals
Anti-Bacterial agents
Antifungal agents
Fitoquímicos
Antibacterianos
Antifúngicos
Composés phytochimiques
Antibactériens
Antifongiques
title_short HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)
title_full HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)
title_fullStr HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)
title_full_unstemmed HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)
title_sort HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae)
author Freitas, Maria Audilene de
author_facet Freitas, Maria Audilene de
Cruz, Rafael Pereira da
Santos, Antonia Thassya Lucas dos
Bezerra, José Weverton Almeida
Machado, Antonio Júdson Targino
Santos, Joycy Francely Sampaio dos
Rocha, Janaina Esmeraldo
Boligon, Aline Augusti
Bezerra, Camila Fonseca
Freitas, Thiago Sampaio de
Silva, Maria Karollyna do Nascimento
Mendonça, Ana Cleide Alcântara Morais
Costa, José Galberto Martins da
Coutinho, Henrique Douglas Melo
Cunha, Francisco Assis Bezerra da
Ribeiro Filho, Jaime
Braga, Maria Flaviana Bezerra Morais
author_role author
author2 Cruz, Rafael Pereira da
Santos, Antonia Thassya Lucas dos
Bezerra, José Weverton Almeida
Machado, Antonio Júdson Targino
Santos, Joycy Francely Sampaio dos
Rocha, Janaina Esmeraldo
Boligon, Aline Augusti
Bezerra, Camila Fonseca
Freitas, Thiago Sampaio de
Silva, Maria Karollyna do Nascimento
Mendonça, Ana Cleide Alcântara Morais
Costa, José Galberto Martins da
Coutinho, Henrique Douglas Melo
Cunha, Francisco Assis Bezerra da
Ribeiro Filho, Jaime
Braga, Maria Flaviana Bezerra Morais
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Freitas, Maria Audilene de
Cruz, Rafael Pereira da
Santos, Antonia Thassya Lucas dos
Bezerra, José Weverton Almeida
Machado, Antonio Júdson Targino
Santos, Joycy Francely Sampaio dos
Rocha, Janaina Esmeraldo
Boligon, Aline Augusti
Bezerra, Camila Fonseca
Freitas, Thiago Sampaio de
Silva, Maria Karollyna do Nascimento
Mendonça, Ana Cleide Alcântara Morais
Costa, José Galberto Martins da
Coutinho, Henrique Douglas Melo
Cunha, Francisco Assis Bezerra da
Ribeiro Filho, Jaime
Braga, Maria Flaviana Bezerra Morais
dc.subject.other.pt_BR.fl_str_mv Compostos fitoquímicos
Antibacterianos
Antifúngicos
topic Compostos fitoquímicos
Antibacterianos
Antifúngicos
Phytochemicals
Anti-Bacterial agents
Antifungal agents
Fitoquímicos
Antibacterianos
Antifúngicos
Composés phytochimiques
Antibactériens
Antifongiques
dc.subject.en.pt_BR.fl_str_mv Phytochemicals
Anti-Bacterial agents
Antifungal agents
dc.subject.es.pt_BR.fl_str_mv Fitoquímicos
Antibacterianos
Antifúngicos
dc.subject.fr.pt_BR.fl_str_mv Composés phytochimiques
Antibactériens
Antifongiques
description Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Federal de Santa Maria. Santa Maria, RS, Brasil. Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-03-11T13:40:54Z
dc.date.available.fl_str_mv 2022-03-11T13:40:54Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Freitas, Maria Audilene de et al. HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae). 3 Biotech, v. 12, n. 1, p. 1-16, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51683
dc.identifier.issn.pt_BR.fl_str_mv 2190-5738
dc.identifier.doi.none.fl_str_mv doi.org/10.1007/s13205-022-03126-1
identifier_str_mv Freitas, Maria Audilene de et al. HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae). 3 Biotech, v. 12, n. 1, p. 1-16, 2022.
2190-5738
doi.org/10.1007/s13205-022-03126-1
url https://www.arca.fiocruz.br/handle/icict/51683
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51683/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51683/2/Freitas%20M%20A%20-%20HPLC-DAD%20analysis.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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