A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters

Detalhes bibliográficos
Autor(a) principal: Oliveira, Viviane Klassen de
Data de Publicação: 2021
Outros Autores: Camargo, Bruno Dias, Alexandrino, Fabiana, Morello, Luis Gustavo, Marchini, Fabrício Klerynton, Aoki, Mateus Nóbrega, Blanes, Lucas
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50024
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
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spelling Oliveira, Viviane Klassen deCamargo, Bruno DiasAlexandrino, FabianaMorello, Luis GustavoMarchini, Fabrício KleryntonAoki, Mateus NóbregaBlanes, Lucas2021-11-24T17:31:57Z2021-11-24T17:31:57Z2021OLIVEIRA, Viviane Klassen de et al. A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters. Biomedical Microdevices, v.23, n.24, p. 1–8, 2021.1572-8781https://www.arca.fiocruz.br/handle/icict/5002410.1007/s10544-021-00563-2porSpringerPCRPolymerase Chain ReactionReagent Kits, DiagnosticReacción en Cadena de la PolimerasaJuego de Reactivos para DiagnósticoRéaction de polymérisation en chaîneTrousses de réactifs pour diagnosticReação em Cadeia da PolimeraseKit de Reagentes para DiagnósticoA low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heatersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.This article describes the fabrication of a low-cost Polymerase Chain Reaction (PCR) instrument to detect diseases. In order to reduce the instrument price and simplify construction we developed an alternative fabrication process, transforming conventional printed circuit boards (PCB) in heating elements, avoiding the use of aluminum heating/cooling blocks and Peltier devices. To cool down the reaction a simple computer fan was used. The vial holder was fabricated using two double side PCB boards assembled in a sandwich-like confguration. The bottom PCB has a resistance of 0.9 Ω used to heat the reaction mix, while the top layer has a resistance of 1.1 Ω to heat the vial body, preventing vapor condensation. The top board was maintained at~110±1 °C during all cycles. The fnal device was able to heat and cool down the reaction at rates of~2.0 °C/s, a rate comparable to commercial thermocyclers. An SMD NTC thermistor was used as temperature sensors, and a PID (proportional–integral–derivative) control algorithm was implemented to acquire and precisely control the temperature. We also discuss how the instrument is calibrated. The device was tested successfully for the amplifcation of T. pallidum (Syphilis) bacterial DNA and Zika virus RNA samples, showing similar performance to a commercial PCR instrument.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/50024/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALOliveira2021_Article_ALow-costPCRInstrumentForMolOK.pdfOliveira2021_Article_ALow-costPCRInstrumentForMolOK.pdfapplication/pdf1085063https://www.arca.fiocruz.br/bitstream/icict/50024/2/Oliveira2021_Article_ALow-costPCRInstrumentForMolOK.pdff58426dd8b0efa65bb68e70eb91b057fMD52icict/500242021-12-13 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dc.title.pt_BR.fl_str_mv A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters
title A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters
spellingShingle A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters
Oliveira, Viviane Klassen de
PCR
Polymerase Chain Reaction
Reagent Kits, Diagnostic
Reacción en Cadena de la Polimerasa
Juego de Reactivos para Diagnóstico
Réaction de polymérisation en chaîne
Trousses de réactifs pour diagnostic
Reação em Cadeia da Polimerase
Kit de Reagentes para Diagnóstico
title_short A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters
title_full A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters
title_fullStr A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters
title_full_unstemmed A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters
title_sort A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters
author Oliveira, Viviane Klassen de
author_facet Oliveira, Viviane Klassen de
Camargo, Bruno Dias
Alexandrino, Fabiana
Morello, Luis Gustavo
Marchini, Fabrício Klerynton
Aoki, Mateus Nóbrega
Blanes, Lucas
author_role author
author2 Camargo, Bruno Dias
Alexandrino, Fabiana
Morello, Luis Gustavo
Marchini, Fabrício Klerynton
Aoki, Mateus Nóbrega
Blanes, Lucas
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Viviane Klassen de
Camargo, Bruno Dias
Alexandrino, Fabiana
Morello, Luis Gustavo
Marchini, Fabrício Klerynton
Aoki, Mateus Nóbrega
Blanes, Lucas
dc.subject.other.pt_BR.fl_str_mv PCR
topic PCR
Polymerase Chain Reaction
Reagent Kits, Diagnostic
Reacción en Cadena de la Polimerasa
Juego de Reactivos para Diagnóstico
Réaction de polymérisation en chaîne
Trousses de réactifs pour diagnostic
Reação em Cadeia da Polimerase
Kit de Reagentes para Diagnóstico
dc.subject.en.pt_BR.fl_str_mv Polymerase Chain Reaction
Reagent Kits, Diagnostic
dc.subject.es.pt_BR.fl_str_mv Reacción en Cadena de la Polimerasa
Juego de Reactivos para Diagnóstico
dc.subject.fr.pt_BR.fl_str_mv Réaction de polymérisation en chaîne
Trousses de réactifs pour diagnostic
dc.subject.decs.pt_BR.fl_str_mv Reação em Cadeia da Polimerase
Kit de Reagentes para Diagnóstico
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-24T17:31:57Z
dc.date.available.fl_str_mv 2021-11-24T17:31:57Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Viviane Klassen de et al. A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters. Biomedical Microdevices, v.23, n.24, p. 1–8, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50024
dc.identifier.issn.pt_BR.fl_str_mv 1572-8781
dc.identifier.doi.none.fl_str_mv 10.1007/s10544-021-00563-2
identifier_str_mv OLIVEIRA, Viviane Klassen de et al. A low‑cost PCR instrument for molecular disease diagnostics based on customized printed circuit board heaters. Biomedical Microdevices, v.23, n.24, p. 1–8, 2021.
1572-8781
10.1007/s10544-021-00563-2
url https://www.arca.fiocruz.br/handle/icict/50024
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50024/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50024/2/Oliveira2021_Article_ALow-costPCRInstrumentForMolOK.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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