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spelling Queiroz, Jackson Alves da SilvaRampazzo, Rita de Cássia PontelloSilva Filho, Edivá Basílio daOliveira, Gabriella SgorlonOliveira, Suyane da CostaSouza, Luan Felipo BotelhoPereira, Soraya dos SantosRodrigues, Moreno Magalhães de SouzaMaia, Adriana Cristina SalvadorSilva, Cicileia Correia daMendonça, Aline Linhares Ferreira de MeloLugtenburg, Celina Aparecida BertoniAguiar, Francisco de Assis AraújoRodrigues, Rosiane de Souza SoaresSantos, Caio Henrique NemethGuimarães, Alice Paula Di SabatinoMáximo, Fernando RodriguesSantos, Alcione de Oliveira dosKrieger, Marco AurélioSalcedo, Juan Miguel VillalobosDall’Acqua, Deusilene Souza Vieira2021-03-09T17:46:38Z2021-03-09T17:46:38Z2021QUEIROZ, Jackson Alves da Silva et al. Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix. International Journal of Infectious Diseases, n. 104, p. 373-378, 2021.1201-9712https://www.arca.fiocruz.br/handle/icict/4631510.1016/j.ijid.2021.01.001porElsevierhttps://www.arca.fiocruz.br/handle/icict/45943Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrixinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-graduação em Biologia Experimental. Porto Velho, RO, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-graduação em Biologia Experimental. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brasil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brasil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brasil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brasil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brasil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brasil.Secretaria de Estado de Finanças de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.Secretaria de Estado da Saúde de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-graduação em Biologia Experimental. Porto Velho, RO, Brasil.Coronavirus disease-2019 (COVID-19) is a disease caused by Severe Acute Respiratory Syndrome Virus 2 (SARS-CoV-2) that emerged in China in late 2019. The rapid viral spread has made the disease a public health emergency of worldwide concern. The gold standard for diagnosing SARS-CoV-2 is reverse transcription followed by qualitative real-time polymerase chain reaction (RT-qPCR); however, the role of viral load quantification has not been thoroughly investigated yet. The aim of this study was to develop a high-precision quantitative one-step RT-qPCR reaction using the association of the viral target and the human target in the same reaction. As methods, the assay standardization involved the absolute quantification method, with serial dilutions of a plasmid with the N gene in a biological matrix to build a standard curve. The results demonstrated the possibility of quantifying as few as 2.5 copies/ reaction and an analysis of 244 patients with known results selected by cross-section that revealed 100% agreement with a qualitative RT-qPCR assay registered by Anvisa. In this population, it was possible to quantify patients with between 2.59 and 3.5 copies per reaction and negative patients continued to indicate the same result. As conclusion, this assay can be a useful tool for a proper patient management, because the level and duration of viral replication are important factors to assess the risk of transmission and to guide decisions regarding the isolation and release of patients; an accurate diagnosis is critical information, whereas the current COVID-19 pandemic represents the biggest current global health problem.SARS-CoV-2Coronavirus InfectionsReal-Time Polymerase Chain ReactionReagent Kits DiagnosticCOVID-19Infecciones por CoronavirusReacción en Cadena en Tiempo Real de la PolimerasaJuego de Reactivos para DiagnósticoInfections à coronavirusRéaction de polymérisation en chaine en temps réelTrousses de réactifs pour diagnosticCOVID-19Infecções por CoronavirusReação em Cadeia da Polimerase em Tempo RealKit de Reagentes para Diagnósticoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix
title Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix
spellingShingle Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix
Queiroz, Jackson Alves da Silva
SARS-CoV-2
Coronavirus Infections
Real-Time Polymerase Chain Reaction
Reagent Kits Diagnostic
COVID-19
Infecciones por Coronavirus
Reacción en Cadena en Tiempo Real de la Polimerasa
Juego de Reactivos para Diagnóstico
Infections à coronavirus
Réaction de polymérisation en chaine en temps réel
Trousses de réactifs pour diagnostic
COVID-19
Infecções por Coronavirus
Reação em Cadeia da Polimerase em Tempo Real
Kit de Reagentes para Diagnóstico
title_short Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix
title_full Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix
title_fullStr Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix
title_full_unstemmed Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix
title_sort Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix
author Queiroz, Jackson Alves da Silva
author_facet Queiroz, Jackson Alves da Silva
Rampazzo, Rita de Cássia Pontello
Silva Filho, Edivá Basílio da
Oliveira, Gabriella Sgorlon
Oliveira, Suyane da Costa
Souza, Luan Felipo Botelho
Pereira, Soraya dos Santos
Rodrigues, Moreno Magalhães de Souza
Maia, Adriana Cristina Salvador
Silva, Cicileia Correia da
Mendonça, Aline Linhares Ferreira de Melo
Lugtenburg, Celina Aparecida Bertoni
Aguiar, Francisco de Assis Araújo
Rodrigues, Rosiane de Souza Soares
Santos, Caio Henrique Nemeth
Guimarães, Alice Paula Di Sabatino
Máximo, Fernando Rodrigues
Santos, Alcione de Oliveira dos
Krieger, Marco Aurélio
Salcedo, Juan Miguel Villalobos
Dall’Acqua, Deusilene Souza Vieira
author_role author
author2 Rampazzo, Rita de Cássia Pontello
Silva Filho, Edivá Basílio da
Oliveira, Gabriella Sgorlon
Oliveira, Suyane da Costa
Souza, Luan Felipo Botelho
Pereira, Soraya dos Santos
Rodrigues, Moreno Magalhães de Souza
Maia, Adriana Cristina Salvador
Silva, Cicileia Correia da
Mendonça, Aline Linhares Ferreira de Melo
Lugtenburg, Celina Aparecida Bertoni
Aguiar, Francisco de Assis Araújo
Rodrigues, Rosiane de Souza Soares
Santos, Caio Henrique Nemeth
Guimarães, Alice Paula Di Sabatino
Máximo, Fernando Rodrigues
Santos, Alcione de Oliveira dos
Krieger, Marco Aurélio
Salcedo, Juan Miguel Villalobos
Dall’Acqua, Deusilene Souza Vieira
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Queiroz, Jackson Alves da Silva
Rampazzo, Rita de Cássia Pontello
Silva Filho, Edivá Basílio da
Oliveira, Gabriella Sgorlon
Oliveira, Suyane da Costa
Souza, Luan Felipo Botelho
Pereira, Soraya dos Santos
Rodrigues, Moreno Magalhães de Souza
Maia, Adriana Cristina Salvador
Silva, Cicileia Correia da
Mendonça, Aline Linhares Ferreira de Melo
Lugtenburg, Celina Aparecida Bertoni
Aguiar, Francisco de Assis Araújo
Rodrigues, Rosiane de Souza Soares
Santos, Caio Henrique Nemeth
Guimarães, Alice Paula Di Sabatino
Máximo, Fernando Rodrigues
Santos, Alcione de Oliveira dos
Krieger, Marco Aurélio
Salcedo, Juan Miguel Villalobos
Dall’Acqua, Deusilene Souza Vieira
dc.subject.en.pt_BR.fl_str_mv SARS-CoV-2
Coronavirus Infections
Real-Time Polymerase Chain Reaction
Reagent Kits Diagnostic
COVID-19
topic SARS-CoV-2
Coronavirus Infections
Real-Time Polymerase Chain Reaction
Reagent Kits Diagnostic
COVID-19
Infecciones por Coronavirus
Reacción en Cadena en Tiempo Real de la Polimerasa
Juego de Reactivos para Diagnóstico
Infections à coronavirus
Réaction de polymérisation en chaine en temps réel
Trousses de réactifs pour diagnostic
COVID-19
Infecções por Coronavirus
Reação em Cadeia da Polimerase em Tempo Real
Kit de Reagentes para Diagnóstico
dc.subject.es.pt_BR.fl_str_mv Infecciones por Coronavirus
Reacción en Cadena en Tiempo Real de la Polimerasa
Juego de Reactivos para Diagnóstico
dc.subject.fr.pt_BR.fl_str_mv Infections à coronavirus
Réaction de polymérisation en chaine en temps réel
Trousses de réactifs pour diagnostic
dc.subject.decs.pt_BR.fl_str_mv COVID-19
Infecções por Coronavirus
Reação em Cadeia da Polimerase em Tempo Real
Kit de Reagentes para Diagnóstico
description Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-graduação em Biologia Experimental. Porto Velho, RO, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-03-09T17:46:38Z
dc.date.available.fl_str_mv 2021-03-09T17:46:38Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv QUEIROZ, Jackson Alves da Silva et al. Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix. International Journal of Infectious Diseases, n. 104, p. 373-378, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46315
dc.identifier.issn.pt_BR.fl_str_mv 1201-9712
dc.identifier.doi.none.fl_str_mv 10.1016/j.ijid.2021.01.001
identifier_str_mv QUEIROZ, Jackson Alves da Silva et al. Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix. International Journal of Infectious Diseases, n. 104, p. 373-378, 2021.
1201-9712
10.1016/j.ijid.2021.01.001
url https://www.arca.fiocruz.br/handle/icict/46315
dc.language.iso.fl_str_mv por
language por
dc.relation.isversionof.pt_BR.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45943
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/46315/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/46315/2/CovidClaudeteok.pdf
https://www.arca.fiocruz.br/bitstream/icict/46315/3/CovidClaudeteok.pdf.txt
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