Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo de conferência |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/17972 |
Resumo: | Fiocruz. Brazilian Ministry of Health. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil |
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Aquino, Carolina LessaLindow, Janet C.Randall, ArloWunder Junior, Elsio AugustoPablo, JozelynNakajima, RieJasinskas, AlgisReis, Mitermayer Galvão dosKo, Albert IcksangFelgner, Philip L.Medeiros, Marco Alberto2017-03-07T18:18:18Z2017-03-07T18:18:18Z2016AQUINO, C. L. et al. Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 52., Alagoas, 2016. Anais eletrônicos... Alagoas: Sociedade Brasileira de Medicina Tropical, 2016. p. 8149.https://www.arca.fiocruz.br/handle/icict/17972porSociedade Brasileira de Medicina TropicalLeptospirosePerfil de anticorposProteínasLeptospirosisAntibody profileProtein microarrayAntibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approachinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectFiocruz. Brazilian Ministry of Health. Bio-Manguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, USAAntigen Discovery Inc. Irvine, CAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, USAUniversity of California Irvine McGaugh Hall. Division of Infectious Disease. Department of Medicine. Irvine, California, USAUniversity of California Irvine McGaugh Hall. Division of Infectious Disease. Department of Medicine. Irvine, California, USAUniversity of California Irvine McGaugh Hall. Division of Infectious Disease. Department of Medicine. Irvine, California, USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, USAUniversity of California Irvine McGaugh Hall. Division of Infectious Disease. Department of Medicine. Irvine, California, USAFiocruz. Brazilian Ministry of Health. Bio-Manguinhos. Rio de Janeiro, RJ, BrasilLeptospirosis is zoonotic disease of global importance, with over a million cases and nearly 60,000 deaths annually. Symptomatic disease presentation ranges from a mild febrile disease with non-specific symptoms to severe forms, characterized by multi-organ failure, lung hemorrhage, and death. Factors governing severe outcomes remain unclear, but the host immune response likely plays an important role. In the present study, we used protein microarray chip to identify the antibody profiles of patients with severe and mild leptospirosis against the complete Leptospira interrogans serovar Copenhageni predicted ORFeome. We discovered a limited number of immunodominant antigens, with 36 antigens specific to patients. Of these, 11 were identified during acute phase, which are therefore potential serodiagnostic antigens while 33 were detected after recovery and are potential subunit vaccine targets. Surprisingly, we found the antibody repertoire varies in patients with different clinical outcomes: in the severe group, overall IgM responses do not change and IgG responses increase over time, while both IgM and IgG responses remain relatively the same in the mild patient group. By analyzing antibody responses by individual patient, we observed that over 74% of patients with severe symptoms compared to 29% of patients with mild leptospirosis had significant IgG increases over time. Additionally, 90.0% of IgM responses did not change over time in the mild group, compared to 51.6% in the severe group. Thus, we hypothesized that patients with mild symptoms were protected from severe disease due to pre-existing antibodies, while the profile of patients with severe outcomes was representative of a first exposure. These findings represent a substantial step forward in the knowledge of the humoral immune response to Leptospira infection, and we have identified new targets for vaccine and diagnostic test development2016-08-21MaceióSociedade Brasileira de Medicina Tropicalbiennialinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17972/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAquino CL 8149-Antibody-profiling-in-patients-with-mild-and-severe-leptospirosis...pdfAquino CL 8149-Antibody-profiling-in-patients-with-mild-and-severe-leptospirosis...pdfapplication/pdf29282https://www.arca.fiocruz.br/bitstream/icict/17972/2/Aquino%20CL%208149-Antibody-profiling-in-patients-with-mild-and-severe-leptospirosis...pdff2fbbe015d46fa71270064edf2df8876MD52TEXTAquino CL 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| dc.title.pt_BR.fl_str_mv |
Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach |
| title |
Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach |
| spellingShingle |
Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach Aquino, Carolina Lessa Leptospirose Perfil de anticorpos Proteínas Leptospirosis Antibody profile Protein microarray |
| title_short |
Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach |
| title_full |
Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach |
| title_fullStr |
Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach |
| title_full_unstemmed |
Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach |
| title_sort |
Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach |
| author |
Aquino, Carolina Lessa |
| author_facet |
Aquino, Carolina Lessa Lindow, Janet C. Randall, Arlo Wunder Junior, Elsio Augusto Pablo, Jozelyn Nakajima, Rie Jasinskas, Algis Reis, Mitermayer Galvão dos Ko, Albert Icksang Felgner, Philip L. Medeiros, Marco Alberto |
| author_role |
author |
| author2 |
Lindow, Janet C. Randall, Arlo Wunder Junior, Elsio Augusto Pablo, Jozelyn Nakajima, Rie Jasinskas, Algis Reis, Mitermayer Galvão dos Ko, Albert Icksang Felgner, Philip L. Medeiros, Marco Alberto |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Aquino, Carolina Lessa Lindow, Janet C. Randall, Arlo Wunder Junior, Elsio Augusto Pablo, Jozelyn Nakajima, Rie Jasinskas, Algis Reis, Mitermayer Galvão dos Ko, Albert Icksang Felgner, Philip L. Medeiros, Marco Alberto |
| dc.subject.other.pt_BR.fl_str_mv |
Leptospirose Perfil de anticorpos Proteínas |
| topic |
Leptospirose Perfil de anticorpos Proteínas Leptospirosis Antibody profile Protein microarray |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospirosis Antibody profile Protein microarray |
| description |
Fiocruz. Brazilian Ministry of Health. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil |
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2016 |
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2016 |
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2017-03-07T18:18:18Z |
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2017-03-07T18:18:18Z |
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AQUINO, C. L. et al. Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 52., Alagoas, 2016. Anais eletrônicos... Alagoas: Sociedade Brasileira de Medicina Tropical, 2016. p. 8149. |
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https://www.arca.fiocruz.br/handle/icict/17972 |
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AQUINO, C. L. et al. Antibody profiling in patients with mild and severe leptospirosis: a genome-wide protein microarray approach. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 52., Alagoas, 2016. Anais eletrônicos... Alagoas: Sociedade Brasileira de Medicina Tropical, 2016. p. 8149. |
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Sociedade Brasileira de Medicina Tropical |
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Sociedade Brasileira de Medicina Tropical |
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