Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.

Detalhes bibliográficos
Autor(a) principal: Aquino, Carolina Lessa
Data de Publicação: 2014
Outros Autores: Wunder Junior, Elsio Augusto, Lindow, Janet C, Rodrigues, Camila B, Pablo, Jozelyn, Nakajima, Rie, Jasinskas, Algis, Liang, Li, Reis, Mitermayer Galvão dos, Ko, Albert Icksang, Medeiros, Marco Alberto, Felgner, Philip L
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10499
Resumo: Fundação Oswaldo Cruz. Bio-Manguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, Brasil / University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, California, USA
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spelling Aquino, Carolina LessaWunder Junior, Elsio AugustoLindow, Janet CRodrigues, Camila BPablo, JozelynNakajima, RieJasinskas, AlgisLiang, LiReis, Mitermayer Galvão dosKo, Albert IcksangMedeiros, Marco AlbertoFelgner, Philip L2015-05-26T18:06:06Z2015-05-26T18:06:06Z2014AQUINO, C. L. et al. Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients. Journal of Proteome Research, v. 14, n. 1, p. 549-556, 2015.1535-3907https://www.arca.fiocruz.br/handle/icict/10499engAmerican Chemical SocietyProteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Bio-Manguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, Brasil / University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, California, USAYale School of Public Health. Department of Epidemiology of Microbial Diseases. College Street. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasilale School of Public Health. Department of Epidemiology of Microbial Diseases. College Street. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Bio-Manguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, Brasil / University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, California, USAYale School of Public Health. Department of Epidemiology of Microbial Diseases. College Street. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilYale School of Public Health. Department of Epidemiology of Microbial Diseases. College Street. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilYale School of Public Health. Department of Epidemiology of Microbial Diseases. College Street. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilYale School of Public Health. Department of Epidemiology of Microbial Diseases. College Street. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / vFundação Oswaldo Cruz. Bio-Manguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, BrasilUniversity of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, California, USAWith increasing efficiency, accuracy, and speed we can access complete genome sequences from thousands of infectious microorganisms; however, the ability to predict antigenic targets of the immune system based on amino acid sequence alone is still needed. Here we use a Leptospira interrogans microarray expressing 91% (3359) of all leptospiral predicted ORFs (3667) and make an empirical accounting of all antibody reactive antigens recognized in sera from naturally infected humans; 191 antigens elicited an IgM or IgG response, representing 5% of the whole proteome. We classified the reactive antigens into 26 annotated COGs (clusters of orthologous groups), 26 JCVI Mainrole annotations, and 11 computationally predicted proteomic features. Altogether, 14 significantly enriched categories were identified, which are associated with immune recognition including mass spectrometry evidence of in vitro expression and in vivo mRNA up-regulation. Together, this group of 14 enriched categories accounts for just 25% of the leptospiral proteome but contains 50% of the immunoreactive antigens. These findings are consistent with our previous studies of other Gram-negative bacteria. This genome-wide approach provides an empirical basis to predict and classify antibody reactive antigens based on structural, physical-chemical, and functional proteomic features and a framework for understanding the breadth and specificity of the immune response to L. interrogans.LeptospirosisProtein microarrayEnrichment analysisAntibody responseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10499/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALAquino CL Proteomic....pdfAquino CL Proteomic....pdfapplication/pdf4322437https://www.arca.fiocruz.br/bitstream/icict/10499/2/Aquino%20CL%20Proteomic....pdf5e115147ceaae2a626c8800b32d4d810MD52TEXTAquino CL Proteomic....pdf.txtAquino CL Proteomic....pdf.txtExtracted texttext/plain47879https://www.arca.fiocruz.br/bitstream/icict/10499/3/Aquino%20CL%20Proteomic....pdf.txt751df5a2c148d31f46acd6143b08ef63MD53icict/104992023-03-15 14:33:20.907oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:20Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.
title Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.
spellingShingle Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.
Aquino, Carolina Lessa
Leptospirosis
Protein microarray
Enrichment analysis
Antibody response
title_short Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.
title_full Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.
title_fullStr Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.
title_full_unstemmed Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.
title_sort Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients.
author Aquino, Carolina Lessa
author_facet Aquino, Carolina Lessa
Wunder Junior, Elsio Augusto
Lindow, Janet C
Rodrigues, Camila B
Pablo, Jozelyn
Nakajima, Rie
Jasinskas, Algis
Liang, Li
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Medeiros, Marco Alberto
Felgner, Philip L
author_role author
author2 Wunder Junior, Elsio Augusto
Lindow, Janet C
Rodrigues, Camila B
Pablo, Jozelyn
Nakajima, Rie
Jasinskas, Algis
Liang, Li
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Medeiros, Marco Alberto
Felgner, Philip L
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Aquino, Carolina Lessa
Wunder Junior, Elsio Augusto
Lindow, Janet C
Rodrigues, Camila B
Pablo, Jozelyn
Nakajima, Rie
Jasinskas, Algis
Liang, Li
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Medeiros, Marco Alberto
Felgner, Philip L
dc.subject.en.pt_BR.fl_str_mv Leptospirosis
Protein microarray
Enrichment analysis
Antibody response
topic Leptospirosis
Protein microarray
Enrichment analysis
Antibody response
description Fundação Oswaldo Cruz. Bio-Manguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, Brasil / University of California Irvine. Department of Medicine. Division of Infectious Disease. Irvine, California, USA
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-26T18:06:06Z
dc.date.available.fl_str_mv 2015-05-26T18:06:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AQUINO, C. L. et al. Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients. Journal of Proteome Research, v. 14, n. 1, p. 549-556, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10499
dc.identifier.issn.none.fl_str_mv 1535-3907
identifier_str_mv AQUINO, C. L. et al. Proteomic features predict seroreactivity against leptospiral antigens in leptospirosis patients. Journal of Proteome Research, v. 14, n. 1, p. 549-556, 2015.
1535-3907
url https://www.arca.fiocruz.br/handle/icict/10499
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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