Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.

Detalhes bibliográficos
Autor(a) principal: Tavares, Natalia Machado
Data de Publicação: 2011
Outros Autores: Silva, Robson Amaro Augusto da, Costa, Dirceu Joaquim, Pitombo, Maiana A, Fukutani, Kiyoshi Ferreira, Miranda, José Carlos, Valenzuela, Jesus G, Barral, Aldina Maria Prado, Oliveira, Camila Indiani de, Barral Netto, Manoel, Brodskyn, Claudia Ida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5862
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Tavares, Natalia MachadoSilva, Robson Amaro Augusto daCosta, Dirceu JoaquimPitombo, Maiana AFukutani, Kiyoshi FerreiraMiranda, José CarlosValenzuela, Jesus GBarral, Aldina Maria PradoOliveira, Camila Indiani deBarral Netto, ManoelBrodskyn, Claudia Ida2012-11-23T17:19:44Z2012-11-23T17:19:44Z2011TAVARES, N.M. et al. Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia. PLoS Neglected Tropical Diseases, v. 5, n. 5, p. e1169, 2011.1935-2735https://www.arca.fiocruz.br/handle/icict/586210.1371/journal.pntd.0001169engLutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto Multidisciplinar em Saúde. Campus Anisio Teixeira. Vitoria da Conquista, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilNational Institute of Allergy and Infectious Disease. National Institutes of Health. Vector Molecular Biology Unit. Rockville, MD, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilLeishmania transmission occurs in the presence of insect saliva. Immunity to Phlebotomus papatasi or Lutzomyia longipalpis saliva or salivary components confers protection against an infection by Leishmania in the presence of the homologous saliva. However, immunization with Lutzomyia intermedia saliva did not protect mice against Leishmania braziliensis plus Lu. intermedia saliva. In the present study, we have studied whether the immunization with Lu. longipalpis saliva or a DNA plasmid coding for LJM19 salivary protein would be protective against L. braziliensis infection in the presence of Lu. intermedia saliva, the natural vector for L. braziliensis. METHODOLOGY/PRINCIPAL FINDINGS: Immunization with Lu. longipalpis saliva or with LJM19 DNA plasmid induced a Delayed-Type Hypersensitivity (DTH) response against Lu. longipalpis as well as against a Lu. intermedia saliva challenge. Immunized and unimmunized control hamsters were then intradermally infected in the ears with L. braziliensis in the presence of Lu. longipalpis or Lu. intermedia saliva. Animals immunized with Lu. longipalpis saliva exhibited smaller lesion sizes as well as reduced disease burdens both at lesion site and in the draining lymph nodes. These alterations were associated with a significant decrease in the expression levels of IL-10 and TGF-ß. Animals immunized with LJM19 DNA plasmid presented similar findings in protection and immune response and additionally increased IFN-γ expression. CONCLUSIONS/SIGNIFICANCE: Immunization with Lu. longipalpis saliva or with a DNA plasmid coding LJM19 salivary protein induced protection in hamsters challenged with L. braziliensis plus Lu. intermedia saliva. These findings point out an important role of immune response against saliva components, suggesting the possibility to develop a vaccine using a single component of Lu. longipalpis saliva to generate protection against different species of Leishmania, even those transmitted by a different vector.Transmissão de Doença Infecciosa/prevenção & controleLeishmania braziliensis/imunologiaLeishmaniose Cutânea/prevenção & controleVacinas Protozoárias/imunologiaPsychodidae/químicaProteínas e Peptídeos Salivares/imunologiaVacinas de DNA/imunologiaAnimaisCricetinaeFemininoInterferon gama/secreçãoInterleucina-10/secreçãoLeishmaniose Cutânea/imunologiaLeishmaniose Cutânea/patologiaLeishmaniose Cutânea/transmissãoLinfonodos/patologiaMasculinoMesocricetusVacinas Protozoárias/administração & dosagemSaliva/químicaSaliva/imunologiaProteínas e Peptídeos Salivares/isolamento & purificaçãoPele/parasitologiaPele/patologiaVacinas de DNA/administração & dosageminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALTavares NM Lutzomyia longipalpis ....pdfTavares NM Lutzomyia longipalpis ....pdfapplication/pdf4137535https://www.arca.fiocruz.br/bitstream/icict/5862/1/Tavares%20NM%20Lutzomyia%20longipalpis%20....pdff61d6a51643d161ac7d3c0cb485c10fcMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5862/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTTavares NM Lutzomyia longipalpis ....pdf.txtTavares NM Lutzomyia longipalpis ....pdf.txtExtracted texttext/plain44956https://www.arca.fiocruz.br/bitstream/icict/5862/5/Tavares%20NM%20Lutzomyia%20longipalpis%20....pdf.txt524258000d2fc0e160bdf1d54f9b2e19MD55THUMBNAILTavares NM Lutzomyia longipalpis ....pdf.jpgTavares NM Lutzomyia longipalpis ....pdf.jpgGenerated Thumbnailimage/jpeg2041https://www.arca.fiocruz.br/bitstream/icict/5862/4/Tavares%20NM%20Lutzomyia%20longipalpis%20....pdf.jpg9bc7faf2380575ff4573b31702bf4896MD54icict/58622023-03-15 14:33:18.148oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.
title Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.
spellingShingle Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.
Tavares, Natalia Machado
Transmissão de Doença Infecciosa/prevenção & controle
Leishmania braziliensis/imunologia
Leishmaniose Cutânea/prevenção & controle
Vacinas Protozoárias/imunologia
Psychodidae/química
Proteínas e Peptídeos Salivares/imunologia
Vacinas de DNA/imunologia
Animais
Cricetinae
Feminino
Interferon gama/secreção
Interleucina-10/secreção
Leishmaniose Cutânea/imunologia
Leishmaniose Cutânea/patologia
Leishmaniose Cutânea/transmissão
Linfonodos/patologia
Masculino
Mesocricetus
Vacinas Protozoárias/administração & dosagem
Saliva/química
Saliva/imunologia
Proteínas e Peptídeos Salivares/isolamento & purificação
Pele/parasitologia
Pele/patologia
Vacinas de DNA/administração & dosagem
title_short Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.
title_full Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.
title_fullStr Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.
title_full_unstemmed Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.
title_sort Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia.
author Tavares, Natalia Machado
author_facet Tavares, Natalia Machado
Silva, Robson Amaro Augusto da
Costa, Dirceu Joaquim
Pitombo, Maiana A
Fukutani, Kiyoshi Ferreira
Miranda, José Carlos
Valenzuela, Jesus G
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
Barral Netto, Manoel
Brodskyn, Claudia Ida
author_role author
author2 Silva, Robson Amaro Augusto da
Costa, Dirceu Joaquim
Pitombo, Maiana A
Fukutani, Kiyoshi Ferreira
Miranda, José Carlos
Valenzuela, Jesus G
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
Barral Netto, Manoel
Brodskyn, Claudia Ida
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tavares, Natalia Machado
Silva, Robson Amaro Augusto da
Costa, Dirceu Joaquim
Pitombo, Maiana A
Fukutani, Kiyoshi Ferreira
Miranda, José Carlos
Valenzuela, Jesus G
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
Barral Netto, Manoel
Brodskyn, Claudia Ida
dc.subject.decs.pt_BR.fl_str_mv Transmissão de Doença Infecciosa/prevenção & controle
Leishmania braziliensis/imunologia
Leishmaniose Cutânea/prevenção & controle
Vacinas Protozoárias/imunologia
Psychodidae/química
Proteínas e Peptídeos Salivares/imunologia
Vacinas de DNA/imunologia
Animais
Cricetinae
Feminino
Interferon gama/secreção
Interleucina-10/secreção
Leishmaniose Cutânea/imunologia
Leishmaniose Cutânea/patologia
Leishmaniose Cutânea/transmissão
Linfonodos/patologia
Masculino
Mesocricetus
Vacinas Protozoárias/administração & dosagem
Saliva/química
Saliva/imunologia
Proteínas e Peptídeos Salivares/isolamento & purificação
Pele/parasitologia
Pele/patologia
Vacinas de DNA/administração & dosagem
topic Transmissão de Doença Infecciosa/prevenção & controle
Leishmania braziliensis/imunologia
Leishmaniose Cutânea/prevenção & controle
Vacinas Protozoárias/imunologia
Psychodidae/química
Proteínas e Peptídeos Salivares/imunologia
Vacinas de DNA/imunologia
Animais
Cricetinae
Feminino
Interferon gama/secreção
Interleucina-10/secreção
Leishmaniose Cutânea/imunologia
Leishmaniose Cutânea/patologia
Leishmaniose Cutânea/transmissão
Linfonodos/patologia
Masculino
Mesocricetus
Vacinas Protozoárias/administração & dosagem
Saliva/química
Saliva/imunologia
Proteínas e Peptídeos Salivares/isolamento & purificação
Pele/parasitologia
Pele/patologia
Vacinas de DNA/administração & dosagem
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-11-23T17:19:44Z
dc.date.available.fl_str_mv 2012-11-23T17:19:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv TAVARES, N.M. et al. Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia. PLoS Neglected Tropical Diseases, v. 5, n. 5, p. e1169, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5862
dc.identifier.issn.none.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001169
identifier_str_mv TAVARES, N.M. et al. Lutzomyia longipalpis saliva or salivary protein LJM19 protects against Leishmania braziliensis and the saliva of its vector, Lutzomyia intermedia. PLoS Neglected Tropical Diseases, v. 5, n. 5, p. e1169, 2011.
1935-2735
10.1371/journal.pntd.0001169
url https://www.arca.fiocruz.br/handle/icict/5862
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