Preservation methods in isolates of Sporothrix characterized by polyphasic approach
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/57361 |
Resumo: | 2023 |
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Rabello, Vanessa Brito de SouzaCorrêa-Moreira, DaniellySantos, CledirPinto, Tatiana Casto AbreuProcopio-Azevedo, Anna CarolinaBoechat, JéssicaCoelho, Rowena AlvesAlmeida-Paes, RodrigoCosta, GiselaLima, NelsonZancopé-Oliveira, Rosely MariaOliveira, Manoel Marques Evangelista2023-03-14T15:28:55Z2023-03-14T15:28:55Z2023RABELLO, Vanessa Brito de Souza et al. Preservation methods in isolates of Sporothrix characterized by polyphasic approach. Journal of Fungi, v. 9, n. 1, p. 1-14, 2023.2309-608Xhttps://www.arca.fiocruz.br/handle/icict/5736110.3390/jof90100342309-608XengMDPIPreservaçãoSporothrixFenótipoGenótipoProteômicaEstabilidadePreservationSporothrixPhenotypeGenotypeProteomicStabilityPreservation methods in isolates of Sporothrix characterized by polyphasic approachinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2023Fundação Oswaldo Cruz. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Mycology. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Evandro Chagas National Institute of Infectious Diseases. Postdoctoral in Clinical Research in Infectious Diseases. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Oswaldo Cruz Institute. Taxonomy, Biochemistry and Bioprospecting of Fungi. Rio de Janeiro, RJ, Brasil.Universidad de La Frontera. Department of Chemistry Science and Natural Resource. Temuco, Cautín, Chile.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Mycology. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals. Rio de Janeiro,RJ, Brasil.Fundação Oswaldo Cruz. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Mycology. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Mycology. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Oswaldo Cruz Institute. Taxonomy, Biochemistry and Bioprospecting of Fungi. Rio de Janeiro, RJ, Brasil.University of Minho. Campus de Gualtar. Biological Engineering Centre. Braga, Portugal / University of Minho, Campus de Gualtar. Associate Laboratory Braga/Guimarães. Braga, Portugal.Fundação Oswaldo Cruz. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Mycology. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Oswaldo Cruz Institute. Taxonomy, Biochemistry and Bioprospecting of Fungi. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Platform for Science, Technology and Innovation in Health. Rio de Janeiro, RJ, Brasil.Sporotrichosis is a subcutaneous mycosis with worldwide distribution and caused by eight pathogenic species of the Sporothrix genus. Different ex situ preservation methods are used around the world to maintain the survival, morphophysiological and genetic traits of fungal strains isolated from patients with sporotrichosis for long terms. The main aim of the present study was to evaluate the survival, phenotypic and genotypic stability of Sporothrix strains after preservation on PDA slant stored at 4 ◦C, sterile water and cryopreservation at −80 ◦C, for a period of time of 6, 12, 18 and 24 months of storage. Eight clinical Sporothrix isolates were identified based on a polyphasic approach consisting of classical macro- and micro-morphological traits, biochemical assays, proteomic profiles by MALDI-TOF MS and molecular biology. According to the final identification, one strain was identified as S. schenckii (CMRVS 40428) and seven strains were re-identified as S. brasiliensis (CMRVS 40421, CMRVS 40423, CMRVS 40424, CMRVS 40425, CMRVS 40426, CMRVS 40427 and CMRVS 40433). In addition, it was observed that the isolates survived after the different time points of storage in distilled water, PDA slant and cryopreservation at −80 ◦C. For fungi preserved in water, low polymorphisms were detected by the partial sequencing of β-tubulin. Cryopreservation at −80 ◦C induced morphological changes in one single isolate. The proteomic profiles obtained by MALDI-TOF MS after preservation showed differences among the methods. In conclusion, preservation on agar slant stored at 4 ◦C was the most effective method to preserve the eight clinical Sporothrix strains. This method produced less change in the phenotypic traits and kept the genetic integrity of all strains. Agar slant stored at 4 ◦C is a simple and inexpensive method and can be especially used in culture collections of limited funding and resources.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57361/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Rabello_Vanessa_etal_INI_2022.pdfve_Rabello_Vanessa_etal_INI_2022.pdfapplication/pdf1454809https://www.arca.fiocruz.br/bitstream/icict/57361/2/ve_Rabello_Vanessa_etal_INI_2022.pdfa1b8337c0e287a3405ddadc707ad5812MD52icict/573612023-09-04 11:30:08.512oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:30:08Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Preservation methods in isolates of Sporothrix characterized by polyphasic approach |
| title |
Preservation methods in isolates of Sporothrix characterized by polyphasic approach |
| spellingShingle |
Preservation methods in isolates of Sporothrix characterized by polyphasic approach Rabello, Vanessa Brito de Souza Preservação Sporothrix Fenótipo Genótipo Proteômica Estabilidade Preservation Sporothrix Phenotype Genotype Proteomic Stability |
| title_short |
Preservation methods in isolates of Sporothrix characterized by polyphasic approach |
| title_full |
Preservation methods in isolates of Sporothrix characterized by polyphasic approach |
| title_fullStr |
Preservation methods in isolates of Sporothrix characterized by polyphasic approach |
| title_full_unstemmed |
Preservation methods in isolates of Sporothrix characterized by polyphasic approach |
| title_sort |
Preservation methods in isolates of Sporothrix characterized by polyphasic approach |
| author |
Rabello, Vanessa Brito de Souza |
| author_facet |
Rabello, Vanessa Brito de Souza Corrêa-Moreira, Danielly Santos, Cledir Pinto, Tatiana Casto Abreu Procopio-Azevedo, Anna Carolina Boechat, Jéssica Coelho, Rowena Alves Almeida-Paes, Rodrigo Costa, Gisela Lima, Nelson Zancopé-Oliveira, Rosely Maria Oliveira, Manoel Marques Evangelista |
| author_role |
author |
| author2 |
Corrêa-Moreira, Danielly Santos, Cledir Pinto, Tatiana Casto Abreu Procopio-Azevedo, Anna Carolina Boechat, Jéssica Coelho, Rowena Alves Almeida-Paes, Rodrigo Costa, Gisela Lima, Nelson Zancopé-Oliveira, Rosely Maria Oliveira, Manoel Marques Evangelista |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Rabello, Vanessa Brito de Souza Corrêa-Moreira, Danielly Santos, Cledir Pinto, Tatiana Casto Abreu Procopio-Azevedo, Anna Carolina Boechat, Jéssica Coelho, Rowena Alves Almeida-Paes, Rodrigo Costa, Gisela Lima, Nelson Zancopé-Oliveira, Rosely Maria Oliveira, Manoel Marques Evangelista |
| dc.subject.other.en_US.fl_str_mv |
Preservação Sporothrix Fenótipo Genótipo Proteômica Estabilidade |
| topic |
Preservação Sporothrix Fenótipo Genótipo Proteômica Estabilidade Preservation Sporothrix Phenotype Genotype Proteomic Stability |
| dc.subject.en.en_US.fl_str_mv |
Preservation Sporothrix Phenotype Genotype Proteomic Stability |
| description |
2023 |
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2023 |
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2023-03-14T15:28:55Z |
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2023-03-14T15:28:55Z |
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2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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RABELLO, Vanessa Brito de Souza et al. Preservation methods in isolates of Sporothrix characterized by polyphasic approach. Journal of Fungi, v. 9, n. 1, p. 1-14, 2023. |
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https://www.arca.fiocruz.br/handle/icict/57361 |
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2309-608X |
| dc.identifier.doi.none.fl_str_mv |
10.3390/jof9010034 |
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2309-608X |
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RABELLO, Vanessa Brito de Souza et al. Preservation methods in isolates of Sporothrix characterized by polyphasic approach. Journal of Fungi, v. 9, n. 1, p. 1-14, 2023. 2309-608X 10.3390/jof9010034 |
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