Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children

Detalhes bibliográficos
Autor(a) principal: Fraga, Thiago Leite
Data de Publicação: 2010
Outros Autores: Brustoloni, Yvone Maia, Lima, Rosimar Baptista, Dorval, Maria Elizabeth Cavalheiros, Oshiro, Elisa Teruya, Oliveira, Janaina, Oliveira, Ana Lúcia Lyrio de, Pirmez, Claude
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25954
Resumo: Universidade Federal de Mato Grosso do Sul. Programa de Pós-graduação em Doenças Infecciosas e Parasitárias. Campo Grande, MS, Brasil.
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spelling Fraga, Thiago LeiteBrustoloni, Yvone MaiaLima, Rosimar BaptistaDorval, Maria Elizabeth CavalheirosOshiro, Elisa TeruyaOliveira, JanainaOliveira, Ana Lúcia Lyrio dePirmez, Claude2018-04-19T14:51:25Z2018-04-19T14:51:25Z2010FRAGA, Thiago Leite; et al. Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children. Mem Inst Oswaldo Cruz, v.105, n.3, p.310-313, May 2010.0074-0276https://www.arca.fiocruz.br/handle/icict/259541678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzLeishmanioseReação em Cadeia da PolimeraseCriançassangue periféricoleishmaniasisperipheral bloodPCRchildrenPolymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in childreninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Mato Grosso do Sul. Programa de Pós-graduação em Doenças Infecciosas e Parasitárias. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Departamento de Pediatria. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunopatalogia. Rio de Janeiro, RJ. Brasil.Universidade Federal de Mato Grosso do Sul. Departamento de Patologia. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Departamento de Patologia. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Programa de Pós-graduação em Doenças Infecciosas e Parasitárias. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Departamento de Pediatria. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunopatalogia. Rio de Janeiro, RJ. Brasil.The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilisation of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25954/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrosimar_lima_etal_IOC_2011.pdfrosimar_lima_etal_IOC_2011.pdfapplication/pdf509365https://www.arca.fiocruz.br/bitstream/icict/25954/2/rosimar_lima_etal_IOC_2011.pdf525a12a95f0300a34723d89670419b11MD52TEXTrosimar_lima_etal_IOC_2011.pdf.txtrosimar_lima_etal_IOC_2011.pdf.txtExtracted texttext/plain25450https://www.arca.fiocruz.br/bitstream/icict/25954/3/rosimar_lima_etal_IOC_2011.pdf.txt74c53fd1bce75202dd9d67956a6a372aMD53icict/259542018-08-15 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dc.title.pt_BR.fl_str_mv Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
title Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
spellingShingle Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
Fraga, Thiago Leite
Leishmaniose
Reação em Cadeia da Polimerase
Crianças
sangue periférico
leishmaniasis
peripheral blood
PCR
children
title_short Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
title_full Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
title_fullStr Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
title_full_unstemmed Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
title_sort Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
author Fraga, Thiago Leite
author_facet Fraga, Thiago Leite
Brustoloni, Yvone Maia
Lima, Rosimar Baptista
Dorval, Maria Elizabeth Cavalheiros
Oshiro, Elisa Teruya
Oliveira, Janaina
Oliveira, Ana Lúcia Lyrio de
Pirmez, Claude
author_role author
author2 Brustoloni, Yvone Maia
Lima, Rosimar Baptista
Dorval, Maria Elizabeth Cavalheiros
Oshiro, Elisa Teruya
Oliveira, Janaina
Oliveira, Ana Lúcia Lyrio de
Pirmez, Claude
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fraga, Thiago Leite
Brustoloni, Yvone Maia
Lima, Rosimar Baptista
Dorval, Maria Elizabeth Cavalheiros
Oshiro, Elisa Teruya
Oliveira, Janaina
Oliveira, Ana Lúcia Lyrio de
Pirmez, Claude
dc.subject.other.pt_BR.fl_str_mv Leishmaniose
Reação em Cadeia da Polimerase
Crianças
sangue periférico
topic Leishmaniose
Reação em Cadeia da Polimerase
Crianças
sangue periférico
leishmaniasis
peripheral blood
PCR
children
dc.subject.en.pt_BR.fl_str_mv leishmaniasis
peripheral blood
PCR
children
description Universidade Federal de Mato Grosso do Sul. Programa de Pós-graduação em Doenças Infecciosas e Parasitárias. Campo Grande, MS, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-04-19T14:51:25Z
dc.date.available.fl_str_mv 2018-04-19T14:51:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRAGA, Thiago Leite; et al. Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children. Mem Inst Oswaldo Cruz, v.105, n.3, p.310-313, May 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25954
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv FRAGA, Thiago Leite; et al. Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children. Mem Inst Oswaldo Cruz, v.105, n.3, p.310-313, May 2010.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/25954
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/25954/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/25954/2/rosimar_lima_etal_IOC_2011.pdf
https://www.arca.fiocruz.br/bitstream/icict/25954/3/rosimar_lima_etal_IOC_2011.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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