Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection

Detalhes bibliográficos
Autor(a) principal: Roma, Eric Henrique
Data de Publicação: 2016
Outros Autores: Macedo, Juan Pereira, Goes, Grazielle Ribeiro, Gonçalves, Juliana Lauar, Castro, Waldionê de, Cisalpino, Daniel, Vieira, Leda Quercia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32489
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
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spelling Roma, Eric HenriqueMacedo, Juan PereiraGoes, Grazielle RibeiroGonçalves, Juliana LauarCastro, Waldionê deCisalpino, DanielVieira, Leda Quercia2019-04-15T14:34:06Z2019-04-15T14:34:06Z2016ROMA, Eric Henrique et al. Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection. Parasites and Vectors, v. 9, p. 1-13, 2016.https://www.arca.fiocruz.br/handle/icict/3248910.1186/s13071-016-1472-y1756-3305engBMCImpact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / National Institutes of Health, NIAID. Laboratory of Malaria and Vector Research. Vector Molecular Biology Section. Rockville, MD, USA.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Background: Reactive oxygen species (ROS) protect the host against a large number of pathogenic microorganisms. ROS have different effects on parasites of the genus Leishmania: some parasites are susceptible to their action, while others seem to be resistant. The role of ROS in L. amazonensis infection in vivo has not been addressed to date. Methods: In this study, C57BL/6 wild-type mice (WT) and mice genetically deficient in ROS production by phagocytes (gp91phox−/−) were infected with metacyclic promastigotes of L. amazonensis to address the effect of ROS in parasite control. Inflammatory cytokines, parasite loads and myeloperoxidase (MPO) activity were evaluated. In parallel, in vitro infection of peritoneal macrophages was assessed to determine parasite killing, cytokine, NO and ROS production. Results: In vitro results show induction of ROS production by infected peritoneal macrophages, but no effect in parasite killing. Also, ROS do not seem to be important to parasite killing in vivo, but they control lesion sizes at early stages of infection. IFN-γ, TNF-α and IL-10 production did not differ among mouse strains. Myeloperoxidase assay showed augmented neutrophils influx 6 h and 72 h post - infection in gp91phox−/− mice, indicating a larger inflammatory response in gp91phox−/− even at early time points. At later time points, neutrophil numbers in lesions correlated with lesion size: larger lesions in gp91phox−/− at earlier times of infection corresponded to larger neutrophil infiltrates, while larger lesions in WT mice at the later points of infection also displayed larger numbers of neutrophils. Conclusion: ROS do not seem to be important in L. amazonensis killing, but they regulate the inflammatory response probably by controlling neutrophils numbers in lesions.Leishmania amazonensisROSNOX2NeutrophilsInflammationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/32489/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Roma_Eric_etal_INI_2016.pdfve_Roma_Eric_etal_INI_2016.pdfapplication/pdf1288523https://www.arca.fiocruz.br/bitstream/icict/32489/2/ve_Roma_Eric_etal_INI_2016.pdf68ff3baf53f2debd05305b49a02c7736MD52TEXTve_Roma_Eric_etal_INI_2016.pdf.txtve_Roma_Eric_etal_INI_2016.pdf.txtExtracted texttext/plain59711https://www.arca.fiocruz.br/bitstream/icict/32489/3/ve_Roma_Eric_etal_INI_2016.pdf.txt38bb3988b4e7573f934e1cb6fa64b195MD53icict/324892019-04-17 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dc.title.pt_BR.fl_str_mv Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection
title Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection
spellingShingle Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection
Roma, Eric Henrique
Leishmania amazonensis
ROS
NOX2
Neutrophils
Inflammation
title_short Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection
title_full Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection
title_fullStr Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection
title_full_unstemmed Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection
title_sort Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection
author Roma, Eric Henrique
author_facet Roma, Eric Henrique
Macedo, Juan Pereira
Goes, Grazielle Ribeiro
Gonçalves, Juliana Lauar
Castro, Waldionê de
Cisalpino, Daniel
Vieira, Leda Quercia
author_role author
author2 Macedo, Juan Pereira
Goes, Grazielle Ribeiro
Gonçalves, Juliana Lauar
Castro, Waldionê de
Cisalpino, Daniel
Vieira, Leda Quercia
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Roma, Eric Henrique
Macedo, Juan Pereira
Goes, Grazielle Ribeiro
Gonçalves, Juliana Lauar
Castro, Waldionê de
Cisalpino, Daniel
Vieira, Leda Quercia
dc.subject.en.pt_BR.fl_str_mv Leishmania amazonensis
ROS
NOX2
Neutrophils
Inflammation
topic Leishmania amazonensis
ROS
NOX2
Neutrophils
Inflammation
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2019-04-15T14:34:06Z
dc.date.available.fl_str_mv 2019-04-15T14:34:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROMA, Eric Henrique et al. Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection. Parasites and Vectors, v. 9, p. 1-13, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32489
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-016-1472-y
dc.identifier.eissn.none.fl_str_mv 1756-3305
identifier_str_mv ROMA, Eric Henrique et al. Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection. Parasites and Vectors, v. 9, p. 1-13, 2016.
10.1186/s13071-016-1472-y
1756-3305
url https://www.arca.fiocruz.br/handle/icict/32489
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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