Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages

Detalhes bibliográficos
Autor(a) principal: Oliveira, Susana de
Data de Publicação: 2017
Outros Autores: Trahamane, Evaristo João da Ordem, Monteiro, Juliana, Santos, Gustavo Pires, Crugeira, Pedro, Sampaio, Fernando, Oliveira, Camila Indiani de, Barral Netto, Manoel, Pinheiro, Antônio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20639
Resumo: Improvement of Higher Education Personnel—CAPES (Post-Doctoral research grant - SCPSO) and the National Council for Scientific and Technological Development—CNPq (MSci Grant - EJOT).
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spelling Oliveira, Susana deTrahamane, Evaristo João da OrdemMonteiro, JulianaSantos, Gustavo PiresCrugeira, PedroSampaio, FernandoOliveira, Camila Indiani deBarral Netto, ManoelPinheiro, Antônio2017-08-16T14:05:07Z2017-08-16T14:05:07Z2017OLIVEIRA, S. et al. Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages. Lasers in Medical Science, jul. 2017.0268-8921https://www.arca.fiocruz.br/handle/icict/2063910.1007/s10103-017-2292-9Improvement of Higher Education Personnel—CAPES (Post-Doctoral research grant - SCPSO) and the National Council for Scientific and Technological Development—CNPq (MSci Grant - EJOT).Federal University of Bahia. Center of Biophotonics. Salvador, BA, BrazilFederal University of Bahia. Center of Biophotonics. Salvador, BA, BrazilFederal University of Bahia. Center of Biophotonics. Salvador, BA, Brazil / Estate University of Feira de Santana. Department of Biology. Feira de Santana, BrazilFederal University of Bahia. Center of Biophotonics. Salvador, BA, BrazilFederal University of Bahia. Center of Biophotonics. Salvador, BA, BrazilFederal University of Bahia. Center of Biophotonics. Salvador, BA, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Center of Biophotonics. Salvador, BA, Brazil / National Institute of Basic Optics and Applied to Life Sciences. São Carlos, SP, BrazilThe aim of this study is to evaluate the effects of ApPDT (antiparasitic photodynamic therapy) on the interaction of Leishmania braziliensis with J774 macrophages, used as a photosensitizer, methylene blue associated with red laser. The tests are in triplicate and the samples divided into four groups: control, photosensitizer, laser, and ApPDT. The photosensitizer used was the methylene blue at concentration of 12.5 μg/mL. The parameters of the laser were λ = 660 nm, 40 mW, and 8.4 J/cm(2). Samples are analyzed by optical microscopy through the identification and counting of infected and uninfected macrophages, parasite load, infectivity, and infection index. Statistical analysis used ANOVA test with Tukey post-test, being considered statistically significant p < 0.05. The analysis of the interaction tests shows that the infection rate in the ApPDT group in relation to the control group presents a statistically significant reduction (p < 0.0001) of 71% at both 24 and 48 h (p < 0.0001) of 62%. ApPDT reduces the number of macrophages infected by Leishmania braziliensis, as well as the number of intracellular parasites, being a possible alternative therapy in the treatment of cutaneous leishmaniosis.engSpringer VerlagLeishmaniaPDTLaserMacrófagosLeishmaniosisPDTLaserMacrophageLeishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophagesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20639/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALOliveira S Leishmanicidal effect....pdfOliveira S Leishmanicidal effect....pdfapplication/pdf851141https://www.arca.fiocruz.br/bitstream/icict/20639/2/Oliveira%20S%20Leishmanicidal%20effect....pdf171f2de4b825ef770ad3ffbc6edebfc2MD52TEXTOliveira S 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dc.title.pt_BR.fl_str_mv Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages
title Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages
spellingShingle Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages
Oliveira, Susana de
Leishmania
PDT
Laser
Macrófagos
Leishmaniosis
PDT
Laser
Macrophage
title_short Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages
title_full Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages
title_fullStr Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages
title_full_unstemmed Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages
title_sort Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages
author Oliveira, Susana de
author_facet Oliveira, Susana de
Trahamane, Evaristo João da Ordem
Monteiro, Juliana
Santos, Gustavo Pires
Crugeira, Pedro
Sampaio, Fernando
Oliveira, Camila Indiani de
Barral Netto, Manoel
Pinheiro, Antônio
author_role author
author2 Trahamane, Evaristo João da Ordem
Monteiro, Juliana
Santos, Gustavo Pires
Crugeira, Pedro
Sampaio, Fernando
Oliveira, Camila Indiani de
Barral Netto, Manoel
Pinheiro, Antônio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Susana de
Trahamane, Evaristo João da Ordem
Monteiro, Juliana
Santos, Gustavo Pires
Crugeira, Pedro
Sampaio, Fernando
Oliveira, Camila Indiani de
Barral Netto, Manoel
Pinheiro, Antônio
dc.subject.other.pt_BR.fl_str_mv Leishmania
PDT
Laser
Macrófagos
topic Leishmania
PDT
Laser
Macrófagos
Leishmaniosis
PDT
Laser
Macrophage
dc.subject.en.pt_BR.fl_str_mv Leishmaniosis
PDT
Laser
Macrophage
description Improvement of Higher Education Personnel—CAPES (Post-Doctoral research grant - SCPSO) and the National Council for Scientific and Technological Development—CNPq (MSci Grant - EJOT).
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-08-16T14:05:07Z
dc.date.available.fl_str_mv 2017-08-16T14:05:07Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, S. et al. Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages. Lasers in Medical Science, jul. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20639
dc.identifier.issn.pt_BR.fl_str_mv 0268-8921
dc.identifier.doi.none.fl_str_mv 10.1007/s10103-017-2292-9
identifier_str_mv OLIVEIRA, S. et al. Leishmanicidal effect of antiparasitic photodynamic therapy-ApPDT on infected macrophages. Lasers in Medical Science, jul. 2017.
0268-8921
10.1007/s10103-017-2292-9
url https://www.arca.fiocruz.br/handle/icict/20639
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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