The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/33677 |
Resumo: | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil. |
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Silva, Djane Clarys Baia daAlvarez, Luis Carlos SalazarLizcano, Omaira VeraCosta, Fabio Trindade MaranhãoLopes, Stefanie Costa PintoOrfanó, Alessandra SilvaPascoal, Denner OliveiraPimenta, Rafael NacifRodriguez, Iria CabralGuerra, Maria das Graças Vale BarbosaLacerda, Marcus Vinicius GuimarãesSecundino, Nagila Francinete CostaMonteiro, Wuelton MarceloPimenta, Paulo Filemon Paolucci2019-06-26T17:35:35Z2019-06-26T17:35:35Z2018SILVA, Djane Clarys Baia da et al. The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis. Parasites and Vectors, v. 11, n. 1, p. 1-10, 2018.1756-3305https://www.arca.fiocruz.br/handle/icict/3367710.1186/s13071-018-2752-5engBioMed CentralQuitinasehematocritoMalariaMatriz peritróficaPlasmodium vivaxTrypsinChitinaseHematocritMalariaPeritrophic matrixPlasmodium vivaxTrypsinThe role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Grupo de Investigación QUIBIO, Departamento de Biología, Universidad Santiago de Cali, Valle del Cauca, Colômbia.Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética, Evolução e Bioagentes. Campinas, SP, Brasil.Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM Brasil / Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.BACKGROUND: Plasmodium vivax is predominant in the Amazon region, and enhanced knowledge of its development inside a natural vector, Anopheles aquasalis, is critical for future strategies aimed at blocking parasite development. The peritrophic matrix (PM), a chitinous layer produced by the mosquito midgut in response to blood ingestion, is a protective barrier against pathogens. Plasmodium can only complete its life-cycle, and consequently be transmitted to a new host, after successfully passing this barrier. Interestingly, fully engorged mosquitoes that had a complete blood meal form a thicker, well-developed PM than ones that feed in small amounts. The amount of red blood cells (RBC) in the blood meal directly influences the production of digestive enzymes and can protect parasites from being killed during the meal digestion. A specific study interrupting the development of the PM associated with the proteolytic activity inhibition, and distinct RBC concentrations, during the P. vivax infection of the New World malaria vector An. aquasalis is expected to clarify whether these factors affect the parasite development. RESULTS: Absence of PM in the vector caused a significant reduction in P. vivax infection. However, the association of chitinase with trypsin inhibitor restored infection rates to those of mosquitoes with a structured PM. Also, only the ingestion of trypsin inhibitor by non-chitinase treated mosquitoes increased the infection intensity. Moreover, the RBC concentration in the infected P. vivax blood meal directly influenced the infection rate and its intensity. A straight correlation was observed between RBC concentrations and infection intensity. CONCLUSIONS: This study established that there is a balance between the PM role, RBC concentration and digestive enzyme activity influencing the establishment and development of P. vivax infection inside An. aquasalis. Our results indicate that the absence of PM in the midgut facilitates digestive enzyme dispersion throughout the blood meal, causing direct damage to P. vivax. On the other hand, high RBC concentrations support a better and thick, well-developed PM and protect P. vivax from being killed. Further studies of this complex system may provide insights into other details of the malaria vector response to P. vivax infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/33677/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALThe role of the peritrophic matrix .pdfThe role of the peritrophic matrix .pdfapplication/pdf10772262https://www.arca.fiocruz.br/bitstream/icict/33677/2/The%20role%20of%20the%20peritrophic%20matrix%20.pdfd36cfaf152937c4735da0d1eb9e0ec02MD52TEXTThe role of the peritrophic matrix .pdf.txtThe role of the peritrophic matrix .pdf.txtExtracted texttext/plain645https://www.arca.fiocruz.br/bitstream/icict/33677/3/The%20role%20of%20the%20peritrophic%20matrix%20.pdf.txt857a31bcc3572b21c28af7775d3b2eb7MD53icict/336772019-06-30 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| dc.title.pt_BR.fl_str_mv |
The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis |
| title |
The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis |
| spellingShingle |
The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis Silva, Djane Clarys Baia da Quitinase hematocrito Malaria Matriz peritrófica Plasmodium vivax Trypsin Chitinase Hematocrit Malaria Peritrophic matrix Plasmodium vivax Trypsin |
| title_short |
The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis |
| title_full |
The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis |
| title_fullStr |
The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis |
| title_full_unstemmed |
The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis |
| title_sort |
The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis |
| author |
Silva, Djane Clarys Baia da |
| author_facet |
Silva, Djane Clarys Baia da Alvarez, Luis Carlos Salazar Lizcano, Omaira Vera Costa, Fabio Trindade Maranhão Lopes, Stefanie Costa Pinto Orfanó, Alessandra Silva Pascoal, Denner Oliveira Pimenta, Rafael Nacif Rodriguez, Iria Cabral Guerra, Maria das Graças Vale Barbosa Lacerda, Marcus Vinicius Guimarães Secundino, Nagila Francinete Costa Monteiro, Wuelton Marcelo Pimenta, Paulo Filemon Paolucci |
| author_role |
author |
| author2 |
Alvarez, Luis Carlos Salazar Lizcano, Omaira Vera Costa, Fabio Trindade Maranhão Lopes, Stefanie Costa Pinto Orfanó, Alessandra Silva Pascoal, Denner Oliveira Pimenta, Rafael Nacif Rodriguez, Iria Cabral Guerra, Maria das Graças Vale Barbosa Lacerda, Marcus Vinicius Guimarães Secundino, Nagila Francinete Costa Monteiro, Wuelton Marcelo Pimenta, Paulo Filemon Paolucci |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Djane Clarys Baia da Alvarez, Luis Carlos Salazar Lizcano, Omaira Vera Costa, Fabio Trindade Maranhão Lopes, Stefanie Costa Pinto Orfanó, Alessandra Silva Pascoal, Denner Oliveira Pimenta, Rafael Nacif Rodriguez, Iria Cabral Guerra, Maria das Graças Vale Barbosa Lacerda, Marcus Vinicius Guimarães Secundino, Nagila Francinete Costa Monteiro, Wuelton Marcelo Pimenta, Paulo Filemon Paolucci |
| dc.subject.other.pt_BR.fl_str_mv |
Quitinase hematocrito Malaria Matriz peritrófica Plasmodium vivax Trypsin |
| topic |
Quitinase hematocrito Malaria Matriz peritrófica Plasmodium vivax Trypsin Chitinase Hematocrit Malaria Peritrophic matrix Plasmodium vivax Trypsin |
| dc.subject.en.pt_BR.fl_str_mv |
Chitinase Hematocrit Malaria Peritrophic matrix Plasmodium vivax Trypsin |
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Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Diretoria de Ensino e Pesquisa. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brasil. |
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2018 |
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2018 |
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2019-06-26T17:35:35Z |
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2019-06-26T17:35:35Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SILVA, Djane Clarys Baia da et al. The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis. Parasites and Vectors, v. 11, n. 1, p. 1-10, 2018. |
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https://www.arca.fiocruz.br/handle/icict/33677 |
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1756-3305 |
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10.1186/s13071-018-2752-5 |
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SILVA, Djane Clarys Baia da et al. The role of the peritrophic matrix and red blood cell concentration in Plasmodium vivax infection of Anopheles aquasalis. Parasites and Vectors, v. 11, n. 1, p. 1-10, 2018. 1756-3305 10.1186/s13071-018-2752-5 |
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BioMed Central |
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BioMed Central |
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