Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative

Detalhes bibliográficos
Autor(a) principal: Silva, Anekécia Lauro da
Data de Publicação: 2014
Outros Autores: Oliveira, Sheilla Andrade de, Oliveira, Jamerson Ferreira de, Santiago, Edna de Farias, Almeida Júnior, Antônio Sérgio Alves, Jacobi, Íris Trindade Tenório, Peixoto, Christina Alves, Rocha, Vinícius Pinto Costa, Soares, Milena Botelho Pereira, Pitta, Ivan da Rocha, Lima, Maria do Carmo Alves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10501
Resumo: Federal University of Pernambuco. Institute of Biology. Department of Antibiotics. Recife, PE, Brasil
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spelling Silva, Anekécia Lauro daOliveira, Sheilla Andrade deOliveira, Jamerson Ferreira deSantiago, Edna de FariasAlmeida Júnior, Antônio Sérgio AlvesJacobi, Íris Trindade TenórioPeixoto, Christina AlvesRocha, Vinícius Pinto CostaSoares, Milena Botelho PereiraPitta, Ivan da RochaLima, Maria do Carmo Alves de2015-05-26T18:50:36Z2015-05-26T18:50:36Z2014SILVA, A. L. et al. Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative. British Journal of Pharmaceutical Research, v. 4, n. 16, p. 1988-2005, 2014.2231-2919https://www.arca.fiocruz.br/handle/icict/10501engSciencedomain InternationalTegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivativeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Pernambuco. Institute of Biology. Department of Antibiotics. Recife, PE, BrasilFundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães. Laboratório of Immunology and Molecular Biology. Recife, PE, BrasilFederal University of Pernambuco. Institute of Biology. Department of Antibiotics. Recife, PE, BrasilFundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães. Laboratório of Immunology and Molecular Biology. Recife, PE, BrasilFederal University of Pernambuco. Institute of Biology. Department of Antibiotics. Recife, PE, BrasilFederal University of Pernambuco. Institute of Biology. Department of Antibiotics. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Laboratório de Ultraestrutura. Departmento de Entomologia. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, BrasilFederal University of Pernambuco. Institute of Biology. Department of Antibiotics. Recife, PE, BrasilFederal University of Pernambuco. Institute of Biology. Department of Antibiotics. Recife, PE, BrasilAims: Verify the potential of the schistosomicidal imidazolidine derivative (5Z)-3-(4- bromo-benzyl)-5-(4-chloro-benzylidene)-4-thioxo-imidazolidin-2-one. Study Design: In this study, we tested the imidazolidinic derivative 3 through in vitro evaluations, cytotoxicity assay and analysis of Scanning Electron Microscopy to verify its therapeutic potential in the treatment of schistosomiasis. Place and Duration of Study: Departamento de Antibióticos, Universidade Federal de Pernambuco (UFPE), Fundação Oswaldo Cruz (FIOCRUZ)/PE and (FIOCRUZ)/BA between January 2013 and march 2014. Methodology: This study was approved by the Ethics Committee on Animal Use Research Center Aggeu Magalhães/Oswaldo Cruz Fundação (CPqAM/FIOCRUZ) authorized by the license No. 21/2011. Male albino Swiss mice were used Mus musculus 25 days old weighing 50 grams. Compound 3 was assayed for its cytotoxicity through cell J774 macrophage lineage. The amount of inhibitory concentration (LC50) was determined by nonlinear regression using the Graph Pad Prism version 5.01. Then the compound was evaluated against adult worms of S. mansoni by performing the activity in vitro at doses 100-20μg/mL and ultrastructural investigation by Scanning Electron Microscopy (SEM) at doses of 100 and 60μg/ml. The PZQ was the positive control of the experiment. Results: The derivative 3 showed LC50 of 29.7±3.9mM. Compound 3 was able to have decreased motility of S. mansoni culminating with a mortality rate of 100% at doses of 60 and 100μg/mL on the fourth day of observation of the experiment. In the SEM, the compound caused various soft tissue changes of S. mansoni parasites such as blistering, destruction of the integument with loss of spines and tubercles, body contraction and windy. Conclusion: The derivative imidazolidine 3 showed a promising schistosomicidal activity in vitro. However, conducting further studies with the completion of work in front of the live schistosomiasis is required.SchistosomiasisImidazolidinesTegumentMicroscopyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10501/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALSilva AL Tegumental....pdfSilva AL Tegumental....pdfapplication/pdf1075054https://www.arca.fiocruz.br/bitstream/icict/10501/2/Silva%20AL%20Tegumental....pdf84c8b45a8a8b55f686120c9ac3597428MD52TEXTSilva AL Tegumental....pdf.txtSilva AL Tegumental....pdf.txtExtracted texttext/plain45331https://www.arca.fiocruz.br/bitstream/icict/10501/3/Silva%20AL%20Tegumental....pdf.txt7139ea70a6c19a375a3d9bbd4a349662MD53icict/105012023-03-15 14:33:11.232oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:11Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative
title Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative
spellingShingle Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative
Silva, Anekécia Lauro da
Schistosomiasis
Imidazolidines
Tegument
Microscopy
title_short Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative
title_full Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative
title_fullStr Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative
title_full_unstemmed Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative
title_sort Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative
author Silva, Anekécia Lauro da
author_facet Silva, Anekécia Lauro da
Oliveira, Sheilla Andrade de
Oliveira, Jamerson Ferreira de
Santiago, Edna de Farias
Almeida Júnior, Antônio Sérgio Alves
Jacobi, Íris Trindade Tenório
Peixoto, Christina Alves
Rocha, Vinícius Pinto Costa
Soares, Milena Botelho Pereira
Pitta, Ivan da Rocha
Lima, Maria do Carmo Alves de
author_role author
author2 Oliveira, Sheilla Andrade de
Oliveira, Jamerson Ferreira de
Santiago, Edna de Farias
Almeida Júnior, Antônio Sérgio Alves
Jacobi, Íris Trindade Tenório
Peixoto, Christina Alves
Rocha, Vinícius Pinto Costa
Soares, Milena Botelho Pereira
Pitta, Ivan da Rocha
Lima, Maria do Carmo Alves de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Anekécia Lauro da
Oliveira, Sheilla Andrade de
Oliveira, Jamerson Ferreira de
Santiago, Edna de Farias
Almeida Júnior, Antônio Sérgio Alves
Jacobi, Íris Trindade Tenório
Peixoto, Christina Alves
Rocha, Vinícius Pinto Costa
Soares, Milena Botelho Pereira
Pitta, Ivan da Rocha
Lima, Maria do Carmo Alves de
dc.subject.en.pt_BR.fl_str_mv Schistosomiasis
Imidazolidines
Tegument
Microscopy
topic Schistosomiasis
Imidazolidines
Tegument
Microscopy
description Federal University of Pernambuco. Institute of Biology. Department of Antibiotics. Recife, PE, Brasil
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-26T18:50:36Z
dc.date.available.fl_str_mv 2015-05-26T18:50:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, A. L. et al. Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative. British Journal of Pharmaceutical Research, v. 4, n. 16, p. 1988-2005, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10501
dc.identifier.issn.none.fl_str_mv 2231-2919
identifier_str_mv SILVA, A. L. et al. Tegumental changes in adult Schistosoma mansoni induced by a new imidazolidinic derivative. British Journal of Pharmaceutical Research, v. 4, n. 16, p. 1988-2005, 2014.
2231-2919
url https://www.arca.fiocruz.br/handle/icict/10501
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sciencedomain International
publisher.none.fl_str_mv Sciencedomain International
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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