Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil

Detalhes bibliográficos
Autor(a) principal: Machado, Lais Ceschini
Data de Publicação: 2019
Outros Autores: Morais-Sobral, Mariana Carolina de, Campos, Tulio de Lima, Pereira, Mylena Ribeiro, Albuquerque, Maria de Fátima Pessoa Militão de, Gilbert, Clément, Franca, Rafael Freitas Oliveira, Wallau, Gabriel Luz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/37962
Resumo: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
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spelling Machado, Lais CeschiniMorais-Sobral, Mariana Carolina deCampos, Tulio de LimaPereira, Mylena RibeiroAlbuquerque, Maria de Fátima Pessoa Militão deGilbert, ClémentFranca, Rafael Freitas OliveiraWallau, Gabriel Luz2019-12-16T13:05:01Z2019-12-16T13:05:01Z2019MACHADO, Lais Ceschini et al. Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. PLoS Neglected Tropical Diseases, v. 13, n. 5, p. 1-15, 2019.1935-2727https://www.arca.fiocruz.br/handle/icict/3796210.1371/journal.pntd.00073321935-2727engGenome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Núcleo de Bioinformática. Recife, PE, Brasil.Universidade Federal Rural de Pernambuco. Departamento de Medicina Veterinária. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Saúde Pública. Recife, PE, Brasil.CNRS Université Paris-Sud. Evolução Laboratorial. Gênomos. Comportamento. Ecologia. Gif sur Yvette, France.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Chikungunya virus (CHIKV) is an RNA virus from the Togaviridae family transmitted by mosquitoes in both sylvatic and urban cycles. In humans, CHIKV infection leads to a febrile illness, denominated Chikungunya fever (CHIKF), commonly associated with more intense and debilitating outcomes. CHIKV arrived in Brazil in 2014 through two independent introductions: the Asian/Caribbean genotype entered through the North region and the African ECSA genotype was imported through the Northeast region. Following their initial introduction, both genotypes established their urban cycle among large naive human populations causing several outbreaks in the Americas. Here, we sequenced CHIKV genomes from a recent outbreak in the Northeast region of Brazil, employing an in-house developed Next-Generation Sequencing (NGS) protocol capable of directly detecting multiple known CHIKV genotypes from clinical positive samples. Our results demonstrate that both Asian/Caribbean and ECSA genotypes expanded their ranges, reaching cocirculation in the Northeast region of Brazil. In addition, our NGS data supports the findings of simultaneous infection by these two genotypes, suggesting that coinfection might be more common than previously thought in highly endemic areas. Future efforts to understand CHIKV epidemiology should thus take into consideration the possibility of coinfection by different genotypes in the human population.Genome sequencingMultiple chikungunya virus genotypesChikungunyaAdultoIdosoBrasil / epidemiologiaFebre de Chikungunya / epidemiologiaFebre de Chikungunya / virologiaVírus Chikungunya / classificaçãoVírus Chikungunya / genéticaVírus Chikungunya / isolamento & purificaçãoCoinfecção / epidemiologiaCoinfecção / virologiaSurtos de DoençasFemininoGenoma ViralGenótipoHumanosMasculinoPessoa de Meia-IdadeFilogeniaPolimorfismo de Nucleotídeo ÚnicoSequenciamento Completo do GenomaAdulto Joveminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83085https://www.arca.fiocruz.br/bitstream/icict/37962/1/license.txt4194663c61da82c21746deb8d6361188MD51ORIGINALGenome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil.pdfGenome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil.pdfapplication/pdf3369746https://www.arca.fiocruz.br/bitstream/icict/37962/2/Genome%20sequencing%20reveals%20coinfection%20by%20multiple%20chikungunya%20virus%20genotypes%20in%20a%20recent%20outbreak%20in%20Brazil.pdfa35e2f3c4178a232234ad3655a8ee55fMD52TEXTGenome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil.pdf.txtGenome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil.pdf.txtExtracted texttext/plain47834https://www.arca.fiocruz.br/bitstream/icict/37962/3/Genome%20sequencing%20reveals%20coinfection%20by%20multiple%20chikungunya%20virus%20genotypes%20in%20a%20recent%20outbreak%20in%20Brazil.pdf.txt48cac200040964a556854b33b974ee90MD53icict/379622020-05-13 21:49:44.216oai:www.arca.fiocruz.br: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Repositório 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dc.title.pt_BR.fl_str_mv Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil
title Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil
spellingShingle Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil
Machado, Lais Ceschini
Genome sequencing
Multiple chikungunya virus genotypes
Chikungunya
Adulto
Idoso
Brasil / epidemiologia
Febre de Chikungunya / epidemiologia
Febre de Chikungunya / virologia
Vírus Chikungunya / classificação
Vírus Chikungunya / genética
Vírus Chikungunya / isolamento & purificação
Coinfecção / epidemiologia
Coinfecção / virologia
Surtos de Doenças
Feminino
Genoma Viral
Genótipo
Humanos
Masculino
Pessoa de Meia-Idade
Filogenia
Polimorfismo de Nucleotídeo Único
Sequenciamento Completo do Genoma
Adulto Jovem
title_short Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil
title_full Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil
title_fullStr Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil
title_full_unstemmed Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil
title_sort Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil
author Machado, Lais Ceschini
author_facet Machado, Lais Ceschini
Morais-Sobral, Mariana Carolina de
Campos, Tulio de Lima
Pereira, Mylena Ribeiro
Albuquerque, Maria de Fátima Pessoa Militão de
Gilbert, Clément
Franca, Rafael Freitas Oliveira
Wallau, Gabriel Luz
author_role author
author2 Morais-Sobral, Mariana Carolina de
Campos, Tulio de Lima
Pereira, Mylena Ribeiro
Albuquerque, Maria de Fátima Pessoa Militão de
Gilbert, Clément
Franca, Rafael Freitas Oliveira
Wallau, Gabriel Luz
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Machado, Lais Ceschini
Morais-Sobral, Mariana Carolina de
Campos, Tulio de Lima
Pereira, Mylena Ribeiro
Albuquerque, Maria de Fátima Pessoa Militão de
Gilbert, Clément
Franca, Rafael Freitas Oliveira
Wallau, Gabriel Luz
dc.subject.en.pt_BR.fl_str_mv Genome sequencing
Multiple chikungunya virus genotypes
Chikungunya
topic Genome sequencing
Multiple chikungunya virus genotypes
Chikungunya
Adulto
Idoso
Brasil / epidemiologia
Febre de Chikungunya / epidemiologia
Febre de Chikungunya / virologia
Vírus Chikungunya / classificação
Vírus Chikungunya / genética
Vírus Chikungunya / isolamento & purificação
Coinfecção / epidemiologia
Coinfecção / virologia
Surtos de Doenças
Feminino
Genoma Viral
Genótipo
Humanos
Masculino
Pessoa de Meia-Idade
Filogenia
Polimorfismo de Nucleotídeo Único
Sequenciamento Completo do Genoma
Adulto Jovem
dc.subject.decs.pt_BR.fl_str_mv Adulto
Idoso
Brasil / epidemiologia
Febre de Chikungunya / epidemiologia
Febre de Chikungunya / virologia
Vírus Chikungunya / classificação
Vírus Chikungunya / genética
Vírus Chikungunya / isolamento & purificação
Coinfecção / epidemiologia
Coinfecção / virologia
Surtos de Doenças
Feminino
Genoma Viral
Genótipo
Humanos
Masculino
Pessoa de Meia-Idade
Filogenia
Polimorfismo de Nucleotídeo Único
Sequenciamento Completo do Genoma
Adulto Jovem
description Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-12-16T13:05:01Z
dc.date.available.fl_str_mv 2019-12-16T13:05:01Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MACHADO, Lais Ceschini et al. Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. PLoS Neglected Tropical Diseases, v. 13, n. 5, p. 1-15, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/37962
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0007332
dc.identifier.eissn.none.fl_str_mv 1935-2727
identifier_str_mv MACHADO, Lais Ceschini et al. Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. PLoS Neglected Tropical Diseases, v. 13, n. 5, p. 1-15, 2019.
1935-2727
10.1371/journal.pntd.0007332
url https://www.arca.fiocruz.br/handle/icict/37962
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
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institution FIOCRUZ
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bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/37962/1/license.txt
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