Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells

Detalhes bibliográficos
Autor(a) principal: Resende, Victor Túlio Ribeiro
Data de Publicação: 2010
Outros Autores: Guimarães, Michelle Lopes Ribeiro, Lemes, Robertha Mariana Rodrigues, Nascimento, Ìsis Cristina, Alves, Lucinéia, Mendez-Otero, Rosalia, Pessolani, Maria Cristina, Lara, Flavio Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26129
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Pavilhão de Hanseníase. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Resende, Victor Túlio RibeiroGuimarães, Michelle Lopes RibeiroLemes, Robertha Mariana RodriguesNascimento, Ìsis CristinaAlves, LucinéiaMendez-Otero, RosaliaPessolani, Maria CristinaLara, Flavio Alves2018-04-26T13:59:45Z2018-04-26T13:59:45Z2010RESENDE, Victor Tulio Ribeiro; et al. Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells. The Journal of Biological Chemistry, v.285, n.44, p.34086-34096, Oct. 2010.0021-9258https://www.arca.fiocruz.br/handle/icict/261291083-351XengAmerican Society for Biochemistry and Molecular BiologyMycobacterium lepraegangliosídeoCélulas de SchwannInfecçãoHanseníaseMycobacterium lepraeInfectionSchwann Cells9-O-acetyl GD3LeprosyInvolvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Pavilhão de Hanseníase. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Pavilhão de Hanseníase. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Pavilhão de Hanseníase. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. BrasilUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Pavilhão de Hanseníase. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. BrasilUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Pavilhão de Hanseníase. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Pavilhão de Hanseníase. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. BrasilMycobacterium leprae (ML), the etiologic agent of leprosy, mainly affects the skin and peripheral nerves, leading to demyelization and loss of axonal conductance. Schwann cells (SCs) are the main cell population infected by ML in the nerves, and infection triggers changes in the SC phenotype from a myelinated to a nonmyelinated state. In the present study, we show that expression of 9-O-acetyl GD3, a ganglioside involved in cellular anti-apoptotic signaling and nerve regeneration, increases in SCs following infection with ML. Observation by confocal microscopy together with coimmunoprecipitation suggested that this ganglioside participates in ML attachment and internalization by SC. Immunoblockage of 9-O-acetyl GD3 in vitro significantly reduced adhesion of ML to SC surfaces. Finally, we show that activation of the MAPK (ERK 1/2) pathway and SC proliferation, two known effects of ML on SCs that result in demyelization, are significantly reduced when the 9-O-acetyl GD3 ganglioside is immunoblocked. Taken together, these data sugges.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26129/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALvictortulio_resende_etal_IOC_2010.pdfvictortulio_resende_etal_IOC_2010.pdfapplication/pdf4017293https://www.arca.fiocruz.br/bitstream/icict/26129/2/victortulio_resende_etal_IOC_2010.pdff088d9c231e4eae1ad1b296861b528a2MD52TEXTvictortulio_resende_etal_IOC_2010.pdf.txtvictortulio_resende_etal_IOC_2010.pdf.txtExtracted texttext/plain53656https://www.arca.fiocruz.br/bitstream/icict/26129/3/victortulio_resende_etal_IOC_2010.pdf.txt0a40c06768a1181efb82187397a69d99MD53icict/261292023-09-05 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dc.title.pt_BR.fl_str_mv Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells
title Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells
spellingShingle Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells
Resende, Victor Túlio Ribeiro
Mycobacterium leprae
gangliosídeo
Células de Schwann
Infecção
Hanseníase
Mycobacterium leprae
Infection
Schwann Cells
9-O-acetyl GD3
Leprosy
title_short Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells
title_full Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells
title_fullStr Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells
title_full_unstemmed Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells
title_sort Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells
author Resende, Victor Túlio Ribeiro
author_facet Resende, Victor Túlio Ribeiro
Guimarães, Michelle Lopes Ribeiro
Lemes, Robertha Mariana Rodrigues
Nascimento, Ìsis Cristina
Alves, Lucinéia
Mendez-Otero, Rosalia
Pessolani, Maria Cristina
Lara, Flavio Alves
author_role author
author2 Guimarães, Michelle Lopes Ribeiro
Lemes, Robertha Mariana Rodrigues
Nascimento, Ìsis Cristina
Alves, Lucinéia
Mendez-Otero, Rosalia
Pessolani, Maria Cristina
Lara, Flavio Alves
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Resende, Victor Túlio Ribeiro
Guimarães, Michelle Lopes Ribeiro
Lemes, Robertha Mariana Rodrigues
Nascimento, Ìsis Cristina
Alves, Lucinéia
Mendez-Otero, Rosalia
Pessolani, Maria Cristina
Lara, Flavio Alves
dc.subject.other.pt_BR.fl_str_mv Mycobacterium leprae
gangliosídeo
Células de Schwann
Infecção
Hanseníase
topic Mycobacterium leprae
gangliosídeo
Células de Schwann
Infecção
Hanseníase
Mycobacterium leprae
Infection
Schwann Cells
9-O-acetyl GD3
Leprosy
dc.subject.en.pt_BR.fl_str_mv Mycobacterium leprae
Infection
Schwann Cells
9-O-acetyl GD3
Leprosy
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Pavilhão de Hanseníase. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-04-26T13:59:45Z
dc.date.available.fl_str_mv 2018-04-26T13:59:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RESENDE, Victor Tulio Ribeiro; et al. Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells. The Journal of Biological Chemistry, v.285, n.44, p.34086-34096, Oct. 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26129
dc.identifier.issn.pt_BR.fl_str_mv 0021-9258
dc.identifier.eissn.none.fl_str_mv 1083-351X
identifier_str_mv RESENDE, Victor Tulio Ribeiro; et al. Involvement of 9-O-Acetyl GD3 Ganglioside in Mycobacterium leprae Infection of Schwann Cells. The Journal of Biological Chemistry, v.285, n.44, p.34086-34096, Oct. 2010.
0021-9258
1083-351X
url https://www.arca.fiocruz.br/handle/icict/26129
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/26129/2/victortulio_resende_etal_IOC_2010.pdf
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