Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania

Detalhes bibliográficos
Autor(a) principal: Telleria, Erich Loza
Data de Publicação: 2021
Outros Autores: Nunes, Bruno Tinoco, Leštinová, Tereza, Avellar, Lívia Monteiro de, Tempone, Antonio Jorge, Pitaluga, André Nóbrega, Volf, Peter, Traub-Csekö, Yara Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/48146
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil / Department of Parasitology. Faculty of Science. Charles University. Prague, Czech Republic.
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spelling Telleria, Erich LozaNunes, Bruno TinocoLeštinová, TerezaAvellar, Lívia Monteiro deTempone, Antonio JorgePitaluga, André NóbregaVolf, PeterTraub-Csekö, Yara Maria2021-07-12T13:48:37Z2021-07-12T13:48:37Z2021TELLERIA, Erich Loza et al. Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania. Microorganims, v. 9, 1271, 15 p, June 2021.2076-2607https://www.arca.fiocruz.br/handle/icict/4814610.3390/microorganisms9061271engMDPILutzomyia longipalpisPeptídeos antimicrobianosImunidade inataSilenciamento genético RNAiLeishmaniaMicrobiotaLutzomyia longipalpisAntimicrobial peptidesInnate immunityRNAi gene silencingLeishmaniaMicrobiotaLutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmaniainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil / Department of Parasitology. Faculty of Science. Charles University. Prague, Czech Republic.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.Department of Parasitology. Faculty of Science. Charles University. Prague, Czech Republic.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.Department of Parasitology. Faculty of Science. Charles University. Prague, Czech Republic.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.Antimicrobial peptides (AMPs) are produced to control bacteria, fungi, protozoa, and other infectious agents. Sand fly larvae develop and feed on a microbe-rich substrate, and the hematophagous females are exposed to additional pathogens. We focused on understanding the role of the AMPs attacin (Att), cecropin (Cec), and four defensins (Def1, Def2, Def3, and Def4) in Lutzomyia longipalpis, the main vector of visceral leishmaniasis in the Americas. Larvae and adults were collected under different feeding regimens, in addition to females artificially infected by Leishmania infantum. AMPs’ gene expression was assessed by qPCR, and gene function of Att and Def2 was investigated by gene silencing. The gene knockdown effect on bacteria and parasite abundance was evaluated by qPCR, and parasite development was verified by light microscopy. We demonstrate that L. longipalpis larvae and adults trigger AMPs expression during feeding, which corresponds to an abundant presence of bacteria. Att and Def2 expression were significantly increased in Leishmania infected females, while Att suppression favored bacteria growth. In conclusion, L. longipalpis AMPs’ expression is tuned in response to bacteria and parasites but does not seem to interfere with the Leishmania cycle.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48146/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALErichTeleria_ViliaVillar_IOC_etal_1921.pdfErichTeleria_ViliaVillar_IOC_etal_1921.pdfapplication/pdf1725556https://www.arca.fiocruz.br/bitstream/icict/48146/2/ErichTeleria_ViliaVillar_IOC_etal_1921.pdf42c67ad1996de36e22da9b9d8908e509MD52TEXTErichTeleria_ViliaVillar_IOC_etal_1921.pdf.txtErichTeleria_ViliaVillar_IOC_etal_1921.pdf.txtExtracted texttext/plain68122https://www.arca.fiocruz.br/bitstream/icict/48146/3/ErichTeleria_ViliaVillar_IOC_etal_1921.pdf.txt57c6a16439250a3d372cc8d4d55c0018MD53icict/481462021-07-13 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dc.title.pt_BR.fl_str_mv Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania
title Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania
spellingShingle Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania
Telleria, Erich Loza
Lutzomyia longipalpis
Peptídeos antimicrobianos
Imunidade inata
Silenciamento genético RNAi
Leishmania
Microbiota
Lutzomyia longipalpis
Antimicrobial peptides
Innate immunity
RNAi gene silencing
Leishmania
Microbiota
title_short Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania
title_full Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania
title_fullStr Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania
title_full_unstemmed Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania
title_sort Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania
author Telleria, Erich Loza
author_facet Telleria, Erich Loza
Nunes, Bruno Tinoco
Leštinová, Tereza
Avellar, Lívia Monteiro de
Tempone, Antonio Jorge
Pitaluga, André Nóbrega
Volf, Peter
Traub-Csekö, Yara Maria
author_role author
author2 Nunes, Bruno Tinoco
Leštinová, Tereza
Avellar, Lívia Monteiro de
Tempone, Antonio Jorge
Pitaluga, André Nóbrega
Volf, Peter
Traub-Csekö, Yara Maria
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Telleria, Erich Loza
Nunes, Bruno Tinoco
Leštinová, Tereza
Avellar, Lívia Monteiro de
Tempone, Antonio Jorge
Pitaluga, André Nóbrega
Volf, Peter
Traub-Csekö, Yara Maria
dc.subject.other.pt_BR.fl_str_mv Lutzomyia longipalpis
Peptídeos antimicrobianos
Imunidade inata
Silenciamento genético RNAi
Leishmania
Microbiota
topic Lutzomyia longipalpis
Peptídeos antimicrobianos
Imunidade inata
Silenciamento genético RNAi
Leishmania
Microbiota
Lutzomyia longipalpis
Antimicrobial peptides
Innate immunity
RNAi gene silencing
Leishmania
Microbiota
dc.subject.en.pt_BR.fl_str_mv Lutzomyia longipalpis
Antimicrobial peptides
Innate immunity
RNAi gene silencing
Leishmania
Microbiota
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil / Department of Parasitology. Faculty of Science. Charles University. Prague, Czech Republic.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-07-12T13:48:37Z
dc.date.available.fl_str_mv 2021-07-12T13:48:37Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TELLERIA, Erich Loza et al. Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania. Microorganims, v. 9, 1271, 15 p, June 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/48146
dc.identifier.issn.pt_BR.fl_str_mv 2076-2607
dc.identifier.doi.none.fl_str_mv 10.3390/microorganisms9061271
identifier_str_mv TELLERIA, Erich Loza et al. Lutzomyia longipalpis Antimicrobial Peptides: Differential Expression during Development and Potential Involvement in Vector Interaction with Microbiota and Leishmania. Microorganims, v. 9, 1271, 15 p, June 2021.
2076-2607
10.3390/microorganisms9061271
url https://www.arca.fiocruz.br/handle/icict/48146
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/48146/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/48146/2/ErichTeleria_ViliaVillar_IOC_etal_1921.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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