Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/27997 |
Resumo: | O Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) concedeu apoio financeiro ao doutorado de M. Paiva-Cavalcanti, que originou o presente estudo. |
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Paiva-Cavalcanti, M.Regis-da-Silva, C.G.Gomes, Y. M.2018-08-08T12:17:39Z2018-08-08T12:17:39Z2010PAIVA-CAVALCANTI, M.; REGIS-DA-SILVA, C.; GOMES, Y. Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 16, n. 4, p. 537–542, 2010.1678-9199https://www.arca.fiocruz.br/handle/icict/2799710.1590/S1678-91992010000400004O Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) concedeu apoio financeiro ao doutorado de M. Paiva-Cavalcanti, que originou o presente estudo.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients. Moreover, the use of a technique that possesses elevated diagnostic sensitivity, and can monitor therapy and prevent relapses promotes broader prospects for the disease control.engDiagnósticoDiagnóstico molecularLeishmaniose visceralDiagnosisMolecular diagnosisVisceral leishmaniasisReação em Cadeia da PolimeraseLeishmaniose Visceral / diagnósticoBrasil / epidemiologiaHumanosSensibilidade e EspecificidadeComparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-reviewinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/27997/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALComparison of real-time PCR and conventional PCR for detection of.pdfComparison of real-time 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| dc.title.pt_BR.fl_str_mv |
Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review |
| title |
Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review |
| spellingShingle |
Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review Paiva-Cavalcanti, M. Diagnóstico Diagnóstico molecular Leishmaniose visceral Diagnosis Molecular diagnosis Visceral leishmaniasis Reação em Cadeia da Polimerase Leishmaniose Visceral / diagnóstico Brasil / epidemiologia Humanos Sensibilidade e Especificidade |
| title_short |
Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review |
| title_full |
Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review |
| title_fullStr |
Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review |
| title_full_unstemmed |
Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review |
| title_sort |
Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review |
| author |
Paiva-Cavalcanti, M. |
| author_facet |
Paiva-Cavalcanti, M. Regis-da-Silva, C.G. Gomes, Y. M. |
| author_role |
author |
| author2 |
Regis-da-Silva, C.G. Gomes, Y. M. |
| author2_role |
author author |
| dc.contributor.author.fl_str_mv |
Paiva-Cavalcanti, M. Regis-da-Silva, C.G. Gomes, Y. M. |
| dc.subject.other.pt_BR.fl_str_mv |
Diagnóstico Diagnóstico molecular Leishmaniose visceral |
| topic |
Diagnóstico Diagnóstico molecular Leishmaniose visceral Diagnosis Molecular diagnosis Visceral leishmaniasis Reação em Cadeia da Polimerase Leishmaniose Visceral / diagnóstico Brasil / epidemiologia Humanos Sensibilidade e Especificidade |
| dc.subject.en.pt_BR.fl_str_mv |
Diagnosis Molecular diagnosis Visceral leishmaniasis |
| dc.subject.decs.pt_BR.fl_str_mv |
Reação em Cadeia da Polimerase Leishmaniose Visceral / diagnóstico Brasil / epidemiologia Humanos Sensibilidade e Especificidade |
| description |
O Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) concedeu apoio financeiro ao doutorado de M. Paiva-Cavalcanti, que originou o presente estudo. |
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2010 |
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2010 |
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2018-08-08T12:17:39Z |
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2018-08-08T12:17:39Z |
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PAIVA-CAVALCANTI, M.; REGIS-DA-SILVA, C.; GOMES, Y. Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 16, n. 4, p. 537–542, 2010. |
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https://www.arca.fiocruz.br/handle/icict/27997 |
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1678-9199 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S1678-91992010000400004 |
| identifier_str_mv |
PAIVA-CAVALCANTI, M.; REGIS-DA-SILVA, C.; GOMES, Y. Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 16, n. 4, p. 537–542, 2010. 1678-9199 10.1590/S1678-91992010000400004 |
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