Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis

Detalhes bibliográficos
Autor(a) principal: Silva, Aline Caroline da
Data de Publicação: 2017
Outros Autores: Santos, Thiago André Ramos dos, Silva, Isis Viviane Bezerra da, Oliveira, Marcos Victor Gregório de, Moreira, Diogo Rodrigo Magalhães, Leite, Ana Cristina Lima, Pereira, Valéria Rêgo Alves
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25614
Resumo: FACEPE, CNPq for financial support and FIOCRUZ for the use of its facilities
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spelling Silva, Aline Caroline daSantos, Thiago André Ramos dosSilva, Isis Viviane Bezerra daOliveira, Marcos Victor Gregório deMoreira, Diogo Rodrigo MagalhãesLeite, Ana Cristina LimaPereira, Valéria Rêgo Alves2018-04-04T17:00:21Z2018-04-04T17:00:21Z2017SILVA, A. C. et al. Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis. Experimental Parasitology, v. 177, p. 57e65, 2017.0014-4894https://www.arca.fiocruz.br/handle/icict/2561410.1016/j.exppara.2017.04.003FACEPE, CNPq for financial support and FIOCRUZ for the use of its facilitiesFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Recife, PE, BrasilUniversidade Federal de Pernambuco. Departamento de Ciências Farmacêticas. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laborat orio de Engenharia Tecidual e Imunofarmacologia. Salvador, BA, BrasilUniversidade Federal de Pernambuco. Departamento de Ciências Farmacêticas. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Recife, PE, BrasilLeishmaniasis is an infection caused by different species of Leishmania genus. Currently, there is no vaccine available for Leishmania infections in humans and conventional treatments are limited due to side effects. Therefore, the development of new antileishmanial drugs is an urgent need. In present study, we evaluated the cytotoxicity in host cells, leishmanicidal activity and immunomodulatory potential of seven aryl thiosemicarbazones. Host cell cytotoxicity was determined in peritoneal macrophages of BALB/c mouse, antiparasitic activity was determined against promastigotes and amastigotes of WHOM/00LTB 0016 strain of L. amazonensis. Nitric oxide (NO) production, interleukin (IL)-12, IL-10 and TNF-alpha secretion were measured in the supernatant of uninfected and infected macrophage cultures. It was observed that aryl thiosemicarbazones presented in vitro antiparasitic activity against both extracellular and intracellular forms of L. amazonensis. However, unlike Amphotericin B, these compounds displayed low cytotoxicity towards host cells. In addition to observed antiparasitic activity, compounds exhibited modulatory properties in the secretion of cytokines and nitrite content from uninfected stimulated and L. amazonensis-infected macrophages. In conclusion, we demonstrated the in vitro antiparasitic activity against L. amazonensis for aryl thiosemicarbazones, which is possible achieved by Th1 cytokine profile modulation. These findings are potential useful for drug development against cutaneous leishmaniasis.porElsevierLeishmaniose cutâneaQuimioterapiaComposto sintéticoThiosemicarbazoneCutaneous leishmaniasisChemotherapySynthetic compoundThiosemicarbazoneAryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25614/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSilva AC Aryl thiosemicarbazones- In vitro....pdfSilva AC Aryl thiosemicarbazones- In vitro....pdfapplication/pdf1207977https://www.arca.fiocruz.br/bitstream/icict/25614/2/Silva%20AC%20Aryl%20thiosemicarbazones-%20In%20vitro....pdfad6cd4cec3cf503b8aa7bca63dec9bddMD52TEXTSilva AC Aryl 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dc.title.pt_BR.fl_str_mv Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis
title Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis
spellingShingle Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis
Silva, Aline Caroline da
Leishmaniose cutânea
Quimioterapia
Composto sintético
Thiosemicarbazone
Cutaneous leishmaniasis
Chemotherapy
Synthetic compound
Thiosemicarbazone
title_short Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis
title_full Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis
title_fullStr Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis
title_full_unstemmed Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis
title_sort Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis
author Silva, Aline Caroline da
author_facet Silva, Aline Caroline da
Santos, Thiago André Ramos dos
Silva, Isis Viviane Bezerra da
Oliveira, Marcos Victor Gregório de
Moreira, Diogo Rodrigo Magalhães
Leite, Ana Cristina Lima
Pereira, Valéria Rêgo Alves
author_role author
author2 Santos, Thiago André Ramos dos
Silva, Isis Viviane Bezerra da
Oliveira, Marcos Victor Gregório de
Moreira, Diogo Rodrigo Magalhães
Leite, Ana Cristina Lima
Pereira, Valéria Rêgo Alves
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Aline Caroline da
Santos, Thiago André Ramos dos
Silva, Isis Viviane Bezerra da
Oliveira, Marcos Victor Gregório de
Moreira, Diogo Rodrigo Magalhães
Leite, Ana Cristina Lima
Pereira, Valéria Rêgo Alves
dc.subject.other.pt_BR.fl_str_mv Leishmaniose cutânea
Quimioterapia
Composto sintético
Thiosemicarbazone
topic Leishmaniose cutânea
Quimioterapia
Composto sintético
Thiosemicarbazone
Cutaneous leishmaniasis
Chemotherapy
Synthetic compound
Thiosemicarbazone
dc.subject.en.pt_BR.fl_str_mv Cutaneous leishmaniasis
Chemotherapy
Synthetic compound
Thiosemicarbazone
description FACEPE, CNPq for financial support and FIOCRUZ for the use of its facilities
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-04-04T17:00:21Z
dc.date.available.fl_str_mv 2018-04-04T17:00:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, A. C. et al. Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis. Experimental Parasitology, v. 177, p. 57e65, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25614
dc.identifier.issn.pt_BR.fl_str_mv 0014-4894
dc.identifier.doi.none.fl_str_mv 10.1016/j.exppara.2017.04.003
identifier_str_mv SILVA, A. C. et al. Aryl thiosemicarbazones: In vitro and immunomodulatory activities against L. amazonensis. Experimental Parasitology, v. 177, p. 57e65, 2017.
0014-4894
10.1016/j.exppara.2017.04.003
url https://www.arca.fiocruz.br/handle/icict/25614
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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